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Microtubules, in vitro /

"There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of t...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Correia, John J. (John Joseph) (Editor ), Wilson, Leslie, 1941- (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam : Academic Press, 2013.
Edición:2nd edition.
Colección:Methods in cell biology ; v. 115.
Temas:
Acceso en línea:Texto completo
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Tabla de Contenidos:
  • Site-specific fluorescent labeling of tubulin / Kamalika Muhkerjee, Susan L. Bane
  • Measuring microtubule persistence length using a microtubule gliding assay / Douglas S. Martin
  • Structural studies of the doublecortin family of MAPs / Frank Fourniol, Myl�ene Perderiset, Anne Houdusse, Carolyn Moores
  • Detection and quantification of microtubule detachment from centrosomes and spindle poles / Anutosh Ganguly, Hailing Yang, Fernando Cabral
  • Regulation of tubulin expression by micro-RNAs : implications for drug resistance / Sharon Lobert, Mary E. Graichen
  • Determining the structure-mechanics relationships of dense microtubule networks with confocal microscopy and magnetic tweezers-based microrheology / Yali Yang, Megan T. Valentine
  • Studying mitochondria and microtubule localization and dynamics in standardized cell shapes / Andrea Pelikan, James Sillibourne, Stephanie Miserey-Lenkei, Frederique Carlier-Grynkorn, Bruno Goud, Phong T. Tran
  • Going solo : measuring the motions of microtubules with an in vitro assay for TIRF microscopy / Kris Leslie, Niels Galjart
  • Analysis of microtubules in isolated axoplasm from the squid giant axon / Yuyu Song, Scott T. Brady
  • Imaging GTP-bound tubulin : from cellular to in vitro assembled microtubules / H�el�ene de Forges, Antoine Pilon, Christian Po�us, Franck Perez
  • Tubulin-specific chaperones : components of a molecular machine that assembles the ... heterodimer / Guoling Tian, Nicholas J. Cowan
  • Heterotrimeric G proteins and microtubules / Witchuda Saengsawang, Mark M. Rasenick
  • Purification and biophysical analysis of microtubule-severing enzymes in vitro / Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L. Ross
  • Measurement of in vitro microtubule polymerization by turbidity and fluorescence / Matthew Mirigian, Kamalika Mukherjee, Susan L. Bane, Dan L. Sackett
  • Live-cell imaging of microtubules and microtubule-associated proteins in arabidopsis thaliana / Jessica Lucas
  • Investigating tubulin posttranslational modifications with specific antibodies / Maria M. Magiera, Carsten Janke
  • Purification and assembly of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences / Jos�e M. Andreu, Mar�ia A. Oliva
  • Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry / P.O. Tsvetkov, P. Barbier, G. Breuzard, V. Peyrot, F. Devred
  • Methods for studying microtubule binding site interactions : zampanolide as a covalent binding agent / Jessica J. Field, Enrique Calvo, Peter T. Northcote, John H. Miller, Karl-Heinz Altmann, Jos�e Fernando D�iaz
  • Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy / Daniel G. Booth, Liam P. Cheeseman, Ian A. Prior, Stephen J. Royle
  • Fluorescence-based assays for microtubule architecture / Susanne Bechstedt, Gary L. Brouhard
  • Structure-function analysis of yeast tubulin / Anna Luchniak, Yusuke Fukuda, Mohan L. Gupta
  • Using MTBindingSim as a tool for experimental planning and interpretation / Julia T. Philip, Aranda R. Duan, Emily O. Alberico, Holly V. Goodson
  • Imaging individual spindle microtubule dynamics in fission yeast / Judite Costa, Chuanhai Fu, Viktoriya Syrovatkina, Phong T. Tran.