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Microtubules, in vitro /

"There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of t...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Correia, John J. (John Joseph) (Editor ), Wilson, Leslie, 1941- (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam : Academic Press, 2013.
Edición:2nd edition.
Colección:Methods in cell biology ; v. 115.
Temas:
Acceso en línea:Texto completo
Texto completo

MARC

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245 0 0 |a Microtubules, in vitro /  |c edited by John J. Correia, Leslie Wilson. 
250 |a 2nd edition. 
264 1 |a Amsterdam :  |b Academic Press,  |c 2013. 
300 |a 1 online resource (xx, 417 pages) :  |b illustrations. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in cell biology ;  |v v. 115 
500 |a Previous edition: 2010. 
588 0 |a Print version record. 
520 |a "There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2nd edition brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors." -- Publisher's description 
505 0 |a Site-specific fluorescent labeling of tubulin / Kamalika Muhkerjee, Susan L. Bane -- Measuring microtubule persistence length using a microtubule gliding assay / Douglas S. Martin -- Structural studies of the doublecortin family of MAPs / Frank Fourniol, Myl�ene Perderiset, Anne Houdusse, Carolyn Moores -- Detection and quantification of microtubule detachment from centrosomes and spindle poles / Anutosh Ganguly, Hailing Yang, Fernando Cabral -- Regulation of tubulin expression by micro-RNAs : implications for drug resistance / Sharon Lobert, Mary E. Graichen -- Determining the structure-mechanics relationships of dense microtubule networks with confocal microscopy and magnetic tweezers-based microrheology / Yali Yang, Megan T. Valentine -- Studying mitochondria and microtubule localization and dynamics in standardized cell shapes / Andrea Pelikan, James Sillibourne, Stephanie Miserey-Lenkei, Frederique Carlier-Grynkorn, Bruno Goud, Phong T. Tran -- Going solo : measuring the motions of microtubules with an in vitro assay for TIRF microscopy / Kris Leslie, Niels Galjart -- Analysis of microtubules in isolated axoplasm from the squid giant axon / Yuyu Song, Scott T. Brady -- Imaging GTP-bound tubulin : from cellular to in vitro assembled microtubules / H�el�ene de Forges, Antoine Pilon, Christian Po�us, Franck Perez -- Tubulin-specific chaperones : components of a molecular machine that assembles the ... heterodimer / Guoling Tian, Nicholas J. Cowan -- Heterotrimeric G proteins and microtubules / Witchuda Saengsawang, Mark M. Rasenick -- Purification and biophysical analysis of microtubule-severing enzymes in vitro / Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L. Ross -- Measurement of in vitro microtubule polymerization by turbidity and fluorescence / Matthew Mirigian, Kamalika Mukherjee, Susan L. Bane, Dan L. Sackett -- Live-cell imaging of microtubules and microtubule-associated proteins in arabidopsis thaliana / Jessica Lucas -- Investigating tubulin posttranslational modifications with specific antibodies / Maria M. Magiera, Carsten Janke -- Purification and assembly of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences / Jos�e M. Andreu, Mar�ia A. Oliva -- Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry / P.O. Tsvetkov, P. Barbier, G. Breuzard, V. Peyrot, F. Devred -- Methods for studying microtubule binding site interactions : zampanolide as a covalent binding agent / Jessica J. Field, Enrique Calvo, Peter T. Northcote, John H. Miller, Karl-Heinz Altmann, Jos�e Fernando D�iaz -- Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy / Daniel G. Booth, Liam P. Cheeseman, Ian A. Prior, Stephen J. Royle -- Fluorescence-based assays for microtubule architecture / Susanne Bechstedt, Gary L. Brouhard -- Structure-function analysis of yeast tubulin / Anna Luchniak, Yusuke Fukuda, Mohan L. Gupta -- Using MTBindingSim as a tool for experimental planning and interpretation / Julia T. Philip, Aranda R. Duan, Emily O. Alberico, Holly V. Goodson -- Imaging individual spindle microtubule dynamics in fission yeast / Judite Costa, Chuanhai Fu, Viktoriya Syrovatkina, Phong T. Tran. 
504 |a Includes bibliographical references and index. 
650 0 |a Microtubules  |x Research  |x Methodology. 
650 0 |a Microtubules. 
650 1 2 |a Microtubules  |0 (DNLM)D008870 
650 6 |a Microtubules  |0 (CaQQLa)201-0014308  |x Recherche  |0 (CaQQLa)201-0380850  |x M�ethodologie.  |0 (CaQQLa)201-0380850 
650 6 |a Microtubules.  |0 (CaQQLa)201-0014308 
650 7 |a SCIENCE  |x Life Sciences  |x Anatomy & Physiology.  |2 bisacsh 
650 7 |a Microtubules  |2 fast  |0 (OCoLC)fst01020141 
655 4 |a Internet Resources. 
655 4 |a Laboratory Manuals. 
700 1 |a Correia, John J.  |q (John Joseph),  |e editor. 
700 1 |a Wilson, Leslie,  |d 1941-  |e editor. 
776 0 8 |i Print version:  |t Microtubules, in vitro.  |b 2nd edition.  |d Amsterdam, Netherlands ; Boston, Mass. : Elsevier/Academic Press, �2013  |z 9780124077577  |w (OCoLC)855703080 
830 0 |a Methods in cell biology ;  |v v. 115. 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/book/9780124077577  |z Texto completo 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/115  |z Texto completo 
880 0 |6 505-00/(S  |a Site-Specific Fluorescent Labeling of Tubulin -- Measuring Microtubule Persistence Length Using a Microtubule Gliding Assay -- Structural Studies of the Doublecortin Family of MAPs -- Detection and Quantification of Microtubule Detachment from Centrosomes and Spindle Poles -- Regulation of Tubulin Expression by Micro-RNAs: Implications for Drug Resistance -- Determining the Structure-Mechanics Relationships of Dense Microtubule Networks with Confocal Microscopy and Magnetic Tweezers-Based Microrheology -- Studying Mitochondria and Microtubule Localization and Dynamics in Standardized Cell Shapes -- Going Solo: Measuring the Motions of Microtubules with an In Vitro Assay for TIRF Microscopy -- Analysis of Microtubules in Isolated Axoplasm from the Squid Giant Axon -- Imaging GTP-Bound Tubulin: From Cellular to In Vitro Assembled Microtubules -- Tubulin-Specific Chaperones: Components of a Molecular Machine That Assembles the α/β Heterodimer -- Heterotrimeric G Proteins and Microtubules -- Purification and Biophysical Analysis of Microtubule-Severing Enzymes In Vitro -- Measurement of In Vitro Microtubule Polymerization by Turbidity and Fluorescence -- Live-Cell Imaging of Microtubules and Microtubule-Associated Proteins in Arabidopsis thaliana -- Investigating Tubulin Posttranslational Modifications with Specific Antibodies -- Purification and Assembly of Bacterial Tubulin BtubA/B and Constructs Bearing Eukaryotic Tubulin Sequences -- Microtubule-Associated Proteins and Tubulin Interaction by Isothermal Titration Calorimetry -- Methods for Studying Microtubule Binding Site Interactions: Zampanolide as a Covalent Binding Agent -- Studying Kinetochore-Fiber Ultrastructure Using Correlative Light-Electron Microscopy -- Fluorescence-Based Assays for Microtubule Architecture -- Structure-Function Analysis of Yeast Tubulin -- Using MTBindingSim as a Tool for Experimental Planning and Interpretation -- Imaging Individual Spindle Microtubule Dynamics in Fission Yeast.