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Adenylyl cyclase, G proteins, and guanylyl cyclase /
This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. Key Features * Adenylyl cyclase: determination of activity * Preparation of materials useful in purification of...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1991.
|
| Colección: | Methods in enzymology ;
v. 195. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
Tabla de Contenidos:
- Assay of adenylyl cyclase catalytic activity / Roger A. Johnson and Yoram Salomon
- Cellular responsiveness to hormones and neutrotransmitters: Conversion of [3H]Adenine to [3H]cAMP in cell monolayers, cell suspensions, and tissue slices / Yoram Salomon
- Preparation of [alpha]-32P-Labeled nucleoside triphosphates, nicotinamide adenine dinucleotide, and cyclic nucleotides for use in determining adenylyl and guanylyl cyclases and cyclic nucleotide phosphodiesterase / Timothy F. Walseth [and others]
- Synthesis of forskolin-agarose affinity matrices / Thomas Pfeuffer
- High-affinity binding sites for [3H]forskolin / Antonio Laurenza and Kenneth B. Seamon
- Purification and characterization of calmodulin-sensitive adenylyl cyclase from bovine brain / Ping Wang and Daniel R. Storm
- Purification of adenylyl cyclase from heart and brain / Elke Pfeuffer [and others]
- Calmodulin-mediated adenylyl cyclase from equine sperm / William A. Toscano, Jr. and Mary K. Gross
- Isolation of polyclonal antibodies against bovine brain calmodulin-sensitive adenylyl cyclases / Gary B. Rosenberg [and others]
- Characteristics and use of monoclonal antibodies to various forms of adenylyl cyclase / Stefan Mollner and Thomas Pfeuffer
- Purification of soluble form of adenylyl cyclase from testes / Theodor Braun
- Purification and assay of cell-invasive form of calmodulin-sensitive adenylyl cyclase from Bordetella pertussis / H. Robert Masure [and others]
- Purification and characterization of adenylyl cyclase from Bacillus anthracis / Stephen H. Leppla
- Preparation and application of GTP-agarose matrices / Elke Pfeuffer and Thomas Pfeuffer
- Purification of G proteins / Juan Codina [and others]
- Preparation of guanine nucleotide-free G proteins / Kenneth M. Ferguson and Tsutomu Higashijima
- Purification of recombinant Gs[alpha] / Michael P. Graziano [and others]
- Purification of recombinant G1[alpha] and Go[alpha] proteins from Escherichia coli / Maurine E. Linder and Alfred G. Gilman
- Synthetic peptide antisera with determined specificity for G protein [alpha] or [beta] subunits / Susanne M. Mumby and Alfred G. Gilman
- Quantitation and purification of ADP-ribosylation factor / Richard A. Kahn
- Soluble guanine nucleotide-dependent ADP-ribosylation factors in activation of adenylyl cyclase by cholera toxin / Joel Moss [and others]
- ADP-ribosylation of G proteins with pertussis toxin / Gregory S. Kopf and Marilyn J. Woolkalis
- Cholera toxin-catalyzed [32P]ADP-ribosylation of proteins / D. Michael Gill and Marilyn J. Woolkalis
- Photoaffinity labeling of GTP-binding proteins / Rolf Thomas and Thomas Pfeuffer.
- Identification of receptor-activated G proteins with photoreactive GTP analog, [[alpha]-32P]GTP azidoanilide / Stefan Offermanns [and others]
- Quantitative immunoblotting of G-protein subunits / Donna J. Carty [and others]
- Assay of g-protein [beta][gamma]-subunit complex by catalytic support of ADP-ribosylation of Go[alpha] / Patrick J. Casey [and others]
- Tryptophan fluorescence of G proteins: Analysis of guanine nucleotide binding and hydrolysis / Tsutomu Higashijima and Kenneth M. Ferguson
- Reconstitution of receptors and G proteins in phospholipid vesicles / Richard A. Cerione and Elliott M. Ross
- Assay of guanylyl cyclase catalytic activity / Steven E. Domino [and others]
- Preparative polyacrylamide gel electrophoresis apparatus for purification of guanylyl cyclase / Alexander M�ulsch and Rupert Gerzer
- Detergent interactions and solubilization techniques for membrane guanylyl cyclase / Arnold A. White and Pushkaraj J. Lad
- Purification of membrane form of guanylyl cyclase / Chodavarapu S. Ramarao and David L. Garbers
- Purification of heme-containing soluble guanylyl cyclase / Alexander M�ulsch and Rupert Gerzer
- Preparation of soluble guanylyl cyclase from bovine lung by immunoaffinity chromatography / Peter Humbert [and others]
- Immunoaffinity purification of soluble guanylyl cyclase / Scott A. Waldman [and others]
- Copurification of atrial natriuretic peptide receptor and particulate guanylyl cyclase / Scott A. Waldman [and others]
- Copurification of atrial natriuretic factor receptors and guanylyl cyclase from adrenal cortex / Tadashi Inagami [and others]
- Molecular cloning of membrane forms of guanylyl cyclase / Sujay Singh and David L. Garbers
- Radiation-inactivation analysis of multidomain proteins: The case of particulate guanylyl cyclase / Michel Potier [and others]
- Identification of atrial natriuretic peptide receptors in cultured cells / Dale C. Leitman [and others]
- Evaluating atrial natriuretic peptide-induced cGMP production by particulate guanylyl cyclase stimulation in Vitro and in Vivo / Pavel Hamet and Johanne Tremblay
- Phosphorylation and dephosphorylation of sea urchin sperm cell guanylyl cyclase / J. Kelley Bentley
- Calcium-regulated guanylyl cyclases from Paramecium and Tetrahymena / Joachim E. Schultz and Suzanne Klumpp.