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Adenylyl cyclase, G proteins, and guanylyl cyclase /

This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. Key Features * Adenylyl cyclase: determination of activity * Preparation of materials useful in purification of...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Johnson, Roger A. (Editor ), Corbin, Jackie D. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1991.
Colección:Methods in enzymology ; v. 195.
Temas:
Acceso en línea:Texto completo
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MARC

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245 0 0 |a Adenylyl cyclase, G proteins, and guanylyl cyclase /  |c edited by Roger A. Johnson, Jackie D. Corbin. 
260 |a San Diego :  |b Academic Press,  |c �1991. 
300 |a 1 online resource (xxx, 512 pages) :  |b illustrations 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in enzymology,  |x 0076-6879 ;  |v v. 195 
504 |a Includes bibliographical references and indexes. 
505 0 0 |t Assay of adenylyl cyclase catalytic activity /  |r Roger A. Johnson and Yoram Salomon --  |t Cellular responsiveness to hormones and neutrotransmitters: Conversion of [3H]Adenine to [3H]cAMP in cell monolayers, cell suspensions, and tissue slices /  |r Yoram Salomon --  |t Preparation of [alpha]-32P-Labeled nucleoside triphosphates, nicotinamide adenine dinucleotide, and cyclic nucleotides for use in determining adenylyl and guanylyl cyclases and cyclic nucleotide phosphodiesterase /  |r Timothy F. Walseth [and others] --  |t Synthesis of forskolin-agarose affinity matrices /  |r Thomas Pfeuffer --  |t High-affinity binding sites for [3H]forskolin /  |r Antonio Laurenza and Kenneth B. Seamon --  |t Purification and characterization of calmodulin-sensitive adenylyl cyclase from bovine brain /  |r Ping Wang and Daniel R. Storm --  |t Purification of adenylyl cyclase from heart and brain /  |r Elke Pfeuffer [and others] --  |t Calmodulin-mediated adenylyl cyclase from equine sperm /  |r William A. Toscano, Jr. and Mary K. Gross --  |t Isolation of polyclonal antibodies against bovine brain calmodulin-sensitive adenylyl cyclases /  |r Gary B. Rosenberg [and others] --  |t Characteristics and use of monoclonal antibodies to various forms of adenylyl cyclase /  |r Stefan Mollner and Thomas Pfeuffer --  |t Purification of soluble form of adenylyl cyclase from testes /  |r Theodor Braun --  |t Purification and assay of cell-invasive form of calmodulin-sensitive adenylyl cyclase from Bordetella pertussis /  |r H. Robert Masure [and others] --  |t Purification and characterization of adenylyl cyclase from Bacillus anthracis /  |r Stephen H. Leppla --  |t Preparation and application of GTP-agarose matrices /  |r Elke Pfeuffer and Thomas Pfeuffer --  |t Purification of G proteins /  |r Juan Codina [and others] --  |t Preparation of guanine nucleotide-free G proteins /  |r Kenneth M. Ferguson and Tsutomu Higashijima --  |t Purification of recombinant Gs[alpha] /  |r Michael P. Graziano [and others] --  |t Purification of recombinant G1[alpha] and Go[alpha] proteins from Escherichia coli /  |r Maurine E. Linder and Alfred G. Gilman --  |t Synthetic peptide antisera with determined specificity for G protein [alpha] or [beta] subunits /  |r Susanne M. Mumby and Alfred G. Gilman --  |t Quantitation and purification of ADP-ribosylation factor /  |r Richard A. Kahn --  |t Soluble guanine nucleotide-dependent ADP-ribosylation factors in activation of adenylyl cyclase by cholera toxin /  |r Joel Moss [and others] --  |t ADP-ribosylation of G proteins with pertussis toxin /  |r Gregory S. Kopf and Marilyn J. Woolkalis --  |t Cholera toxin-catalyzed [32P]ADP-ribosylation of proteins /  |r D. Michael Gill and Marilyn J. Woolkalis --  |t Photoaffinity labeling of GTP-binding proteins /  |r Rolf Thomas and Thomas Pfeuffer. 
505 0 0 |t Identification of receptor-activated G proteins with photoreactive GTP analog, [[alpha]-32P]GTP azidoanilide /  |r Stefan Offermanns [and others] --  |t Quantitative immunoblotting of G-protein subunits /  |r Donna J. Carty [and others] --  |t Assay of g-protein [beta][gamma]-subunit complex by catalytic support of ADP-ribosylation of Go[alpha] /  |r Patrick J. Casey [and others] --  |t Tryptophan fluorescence of G proteins: Analysis of guanine nucleotide binding and hydrolysis /  |r Tsutomu Higashijima and Kenneth M. Ferguson --  |t Reconstitution of receptors and G proteins in phospholipid vesicles /  |r Richard A. Cerione and Elliott M. Ross --  |t Assay of guanylyl cyclase catalytic activity /  |r Steven E. Domino [and others] --  |t Preparative polyacrylamide gel electrophoresis apparatus for purification of guanylyl cyclase /  |r Alexander M�ulsch and Rupert Gerzer --  |t Detergent interactions and solubilization techniques for membrane guanylyl cyclase /  |r Arnold A. White and Pushkaraj J. Lad --  |t Purification of membrane form of guanylyl cyclase /  |r Chodavarapu S. Ramarao and David L. Garbers --  |t Purification of heme-containing soluble guanylyl cyclase /  |r Alexander M�ulsch and Rupert Gerzer --  |t Preparation of soluble guanylyl cyclase from bovine lung by immunoaffinity chromatography /  |r Peter Humbert [and others] --  |t Immunoaffinity purification of soluble guanylyl cyclase /  |r Scott A. Waldman [and others] --  |t Copurification of atrial natriuretic peptide receptor and particulate guanylyl cyclase /  |r Scott A. Waldman [and others] --  |t Copurification of atrial natriuretic factor receptors and guanylyl cyclase from adrenal cortex /  |r Tadashi Inagami [and others] --  |t Molecular cloning of membrane forms of guanylyl cyclase /  |r Sujay Singh and David L. Garbers --  |t Radiation-inactivation analysis of multidomain proteins: The case of particulate guanylyl cyclase /  |r Michel Potier [and others] --  |t Identification of atrial natriuretic peptide receptors in cultured cells /  |r Dale C. Leitman [and others] --  |t Evaluating atrial natriuretic peptide-induced cGMP production by particulate guanylyl cyclase stimulation in Vitro and in Vivo /  |r Pavel Hamet and Johanne Tremblay --  |t Phosphorylation and dephosphorylation of sea urchin sperm cell guanylyl cyclase /  |r J. Kelley Bentley --  |t Calcium-regulated guanylyl cyclases from Paramecium and Tetrahymena /  |r Joachim E. Schultz and Suzanne Klumpp. 
588 0 |a Print version record. 
520 |a This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. Key Features * Adenylyl cyclase: determination of activity * Preparation of materials useful in purification of components of hormonally responsive cyclase systems * Purification guanine nucleotide-dependent regulatory proteins * Purification and characterization of g proteins: labeling and quantitating of G proteins * Reconstitution guanylyl cyclase: assay * Preparation of materials used for study of the cyclase * Purification * Regulation. 
546 |a English. 
650 0 |a Adenylate cyclase. 
650 0 |a G proteins. 
650 2 |a Adenylyl Cyclases  |x isolation & purification  |0 (DNLM)D000262Q000302 
650 2 |a GTP-Binding Proteins  |x isolation & purification  |0 (DNLM)D019204Q000302 
650 2 |a Guanylyl Imidodiphosphate  |x isolation & purification  |0 (DNLM)D006165Q000302 
650 2 |a Adenylyl Cyclases  |0 (DNLM)D000262 
650 2 |a GTP-Binding Proteins  |0 (DNLM)D019204 
650 6 |a Ad�enylcyclase.  |0 (CaQQLa)201-0085522 
650 6 |a Prot�eines G.  |0 (CaQQLa)201-0203891 
650 7 |a Adenylate cyclase  |2 fast  |0 (OCoLC)fst00796589 
650 7 |a G proteins  |2 fast  |0 (OCoLC)fst00936830 
700 1 |a Johnson, Roger A.,  |e editor. 
700 1 |a Corbin, Jackie D.,  |e editor. 
776 0 8 |i Print version:  |t Adenylyl cyclase, G proteins, and guanylyl cyclase.  |d San Diego : Academic Press, �1991  |z 0121820963  |w (DLC) 54009110  |w (OCoLC)24794443 
830 0 |a Methods in enzymology ;  |v v. 195.  |x 0076-6879 
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856 4 0 |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/195  |z Texto completo 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879  |z Texto completo