Cell biology : a laboratory handbook /

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This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Celis, J. E. (Julio E.)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Boston : Elsevier Academic, �2006.
Edición:3rd ed.
Temas:
Cytology > Laboratory manuals.
Cytology > Technique.
Cultures (Biology)
Cytological Techniques
Culture Techniques
Laboratory Manuals
Cytologie > Manuels de laboratoire.
Cytologie > Technique.
Cultures (Biologie)
SCIENCE > Life Sciences > Cell Biology.
Cytology > Technique
Cytology
Cytologie.
Technique de laboratoire.
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Contents of Volume 1:
  • Part A. Cell and Tissue Culture: Associated Techniques.
  • Section 1. General Techniques.
  • Section 2. Culture of Specific Cell Types: Stem Cells.
  • Section 3. Culture of Specific Cell Types: Haemopoietic, Mesenchymal, and Epithelial.
  • Section 4. Differentiation and Reprogramming of Somatic Cells.
  • Section 5. Immortalisation.
  • Section 6. Somatic Cell Hybrids.
  • Section 7. Cell Separation Techniques.
  • Section 8. Cell Cycle Analysis.
  • Section 9. Cytotoxic and Cell Growth Assays.
  • Section 10. Apoptosis.
  • Section 11. Assays of Cell Transformation, Tumorigenesis, Invasion and Wound Healing.
  • Section 12. Electrophysiological Methods.
  • Section 13. Organ Cultures.
  • Part B. Viruses.
  • Section 14. Growth and Purification of Viruses.
  • Part C. Antibodies.
  • Section 15. Production and Purification of Antibodies.
  • Part D. Immunocytochemistry.
  • Section 16. Immunofluoresence.
  • Part E. Appendix.
  • Contents of Volume 2:
  • Part A. Organelles and Cellular Structures.
  • Section 1. Isolation: Plasma Membrane, Organelles, and Cellular Structures.
  • Section 2. Vital Staining of Cells/Organelles.
  • Section 3. Protein Purification.
  • Part B. Assays.
  • Section 4. Endocytic and Exocytic Pathways.
  • Section 5. Membranes.
  • Section 6. Mitochondria.
  • Section 7. Nuclear Transport.
  • Section 8. Chromatin Assembly.
  • Section 9. Signal Transduction Assays.
  • Section 10. Assays and Models of in Vitro and in Vitro Motility.
  • Section 11. Mechanical Stress in Single Cells.
  • Part C. Appendix.
  • Contents of Volume 3.
  • Part A. Imaging Techniques.
  • Section 1. Light Microscopy.
  • Section 2. Digital Video Microscopy.
  • Section 3. Confocal Microscopy of Living Cells and Fixed Cells.
  • Section 4. Fluorescent Microscopy of Living Cells.
  • Section 5. Use of Fluorescent Dyes for Studies of Intracellular Physiological Parameters.
  • Section 6. Digital Image Processing, Analysis, Storage, and Display.
  • Part B. Electron Microscopy.
  • Section 7. Specimen Preparation Techniques.
  • Section 8. Cryotechniques.
  • Section 9. Electron Microscopy Studies of the Cytoskeleton.
  • Section 10. Immunoelectron Microscopy.
  • Part C. Scanning Probe and Scanning Electron Microscopy.
  • Section 11. Scanning Probe and Scanning Electron Microscopy.
  • Part D. Microdissection.
  • Section 12. Tissue and Chromosome Microdissection.
  • Part E. Tissue Arrays.
  • Section 13. Tissue Arrays.
  • Part F. Cytogenetics and in Situ Hybridisation
  • Section 14. Cytogenetics.
  • Section 15. In Situ Hybridisation.
  • Part G. Genomics.
  • Section 16. Genomics.
  • Part H. Transgenic, Knockouts, and Knockdown Methods.
  • Section 17. Transgenic, Knockouts and knock-down methods.
  • Contents of Volume 4.
  • Part A. Transfer of Macromolecules.
  • Section 1. Proteins.
  • Section 2. Genes.
  • Section 3. Somatic Cell Nuclear Transfer.
  • Part B. Expression Systems.
  • Section 4. Expression Systems.
  • Part C. Gene Expression Profiling.
  • Section 5. Differential Gene Expression.
  • Part D. Proteins.
  • Section 6. Protein Determination and Analysis.
  • Section 7. Sample Fractionation for Proteomics.
  • Section 8. gel Electrophoresis.
  • Section 9. Detection of Proteins in Gels.
  • Section 10. Gel Profiling of Posttranslationally Modified Proteins.
  • Section 11. Protein/Protein and Protein/Small Molecule Interactions.
  • Section 12. Functional Proteomics.
  • Section 13. Protein/DNA Interactions.
  • Section 14. Protein Degradation.
  • Section 15. Mass Spectrometry: Protein Identification and Interactions.
  • Part E. Appendix.

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