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Thiol redox transitions in cell signaling. Part A, Chemistry and biochemistry of low molecular weight and protein thiols /

This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This firstinstallment of Cadenas and Packer's two-volume treatment specifically deals with glutathionylati...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Cadenas, Enrique, Packer, Lester
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam, Netherlands ; Boston, Mass. : Elsevier/Academic, �2010.
Colección:Methods in enzymology ; volume 473.
Temas:
Acceso en línea:Texto completo
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Tabla de Contenidos:
  • Changing paradigms in thiology: from antioxidant defense toward redox regulation / Leopold Floh�e
  • Mass spectrometry-based methods for the determination of sulfur and related metabolite concentrations in cell extracts / Emmanuel Godat [and others]
  • Use of dimedone-based chemical probes for sulfenic acid detection: evaluation of conditions affecting probe incorporation into redox-sensitive proteins / Chananat Klomsiri [and others]
  • Use of dimedone-based chemical probes for sulfenic acid detection: methods to visualize and identify labeled proteins / Kimberly J. Nelson [and others]
  • Formation and reactions of sulfenic acid in human serum albumin / Beatriz Alvarez [and others]
  • Determination of GSH, GSSG, and GSNO using HPLC with electrochemical detection / Li-Peng Yap [and others]
  • Measurement of mixed disulfides including glutathionylated proteins / Raffaella Priora [and others]
  • Detection and quantification of protein disulfides in biological tissues: a fluorescence-based proteomic approach / Viviana I. P�erez [and others]
  • Measurement and identification of S-glutathiolated proteins / Bradford G. Hill [and others]
  • Proteome screens for Cys residues oxidation: the redoxome / Giovanni Chiappetta [and others]
  • Identification by MS/MS of disulfides produced by a functional redox transition / Pierluigi Mauri [and others]
  • Mass spectrometry approaches for the redox characterization of protein cysteine residues: the case of the transcription factor Pax-8 / Andrea Scaloni and Gianluca Tell
  • A simple method to systematically study oxidatively modified proteins in biological samples and its applications / Byoung-Joon Song, Soo-Kyung Suh and Kwan-Hoon Moon
  • Direct and indirect detection methods for the analysis of S-nitrosylated peptides and proteins / Federico Torta, Lisa Elviri and Angela Bachi
  • A rapid approach for the detection, quantification, and discovery of novel sulfenic acid or S-nitrosothiol modified proteins using a biotin-switch method / Joseph R. Burgoyne and Philip Eaton
  • Protein addicts of aldehydic lipid peroxidation products: identification and characterization of protein adducts using an aldehyde/keto-reactive probe in combination with mass spectrometry / Claudia S. Maier [and others].