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Phage Therapy. Part A /

Detalles Bibliográficos
Clasificación:	Libro Electrónico
Otros Autores:	Singh, Vijai (Editor )
Formato:	Electrónico eBook
Idioma:	Inglés
Publicado:	Cambridge, MA : Academic Press, 2023.
Colección:	Progress in molecular biology and translational science ; 200.
Temas:	Molecular biology. Bacteriophages.
Acceso en línea:	Texto completo

Tabla de Contenidos:

Front Cover
Title page
Copyright
Contents
Contributors
Praface
Chapter One: Exploring the potential of phage and their applications
1 Introduction
2 Life cycle of phage
3 Applications of phage
3.1 Phage for detection of bacterial pathogens
3.2 Phage therapy for bacterial infections
3.3 Phage in food industry
3.4 Phage clinical trails
3.5 Directed evolution and optimization of phage for enhanced therapy
4 Conclusion and future remarks
Acknowledgements
References
Chapter Two: Isolation, screening and characterization of phageIsolation, screening and characterization of phage
1 Introduction
2 Become a phage-hunter: phage isolation
2.1 Sources for phage isolation
2.2 General scheme for phage isolation, with emphasis on jumbo phage
2.3 Biased and unbiased isolation methods in phage isolation with highlight on advanced techniques
2.4 Purification and amplification
3 Phage characterization
3.1 Phage screening
3.1.1 Host range
3.1.1.1 Solid-based methods
3.1.1.1.1 Spot test for determination of phage host range.
3.1.1.1.2 Relative efficiency of platting
3.1.1.2 Liquid-based methods
3.1.1.2.1 Microtiter plate host range assay.
3.1.1.2.2 High-throughput turbidimetric test.
3.1.2 Phage adsorption
3.2 Morphological characterization
3.2.1 Phage plaque morphology
3.2.2 Virion structure morphology
3.2.2.1 Transmission electron microscopy
3.2.2.2 Scanning electron microscopy
3.2.2.3 Atomic force microscopy
3.2.2.4 Cryo-electron microscopy
3.3 Biological characterization
3.3.1 One-step growth curve
3.3.2 Physical and chemical stability
3.3.2.1 Temperature stability
3.3.2.2 UV stability
3.3.2.3 Chemical stability
3.3.2.3.1 pH stability.
3.3.2.3.2 Salinity and ions
4 Genomic characterization.
4.1 Phage genome from extraction to annotation
4.2 Further screening of the phage genome
4.2.1 Safety
4.2.2 Temperate lifecycle gene-markers
4.2.3 tRNA and ncRNA genes
4.2.4 Holin-lysin lysis system
4.2.5 Polysaccharide depolymerases
4.2.6 Phage packaging mechanism
4.2.7 Regulator elements: promoters and terminators
4.3 Phage genome visualization and comparison
4.4 Phage phylogenetic analysis
4.4.1 At family level
4.4.2 At genus and species levels
5 Conclusion and future remarks
References
Further reading
Chapter Three: Synthetic phage and its application in phage therapy
1 Introduction
2 Structure
2.1 Composition
2.2 Morphology
3 Classification
4 Development of synthetic phage
4.1 Homological remerging (HR)
4.2 Genetic data rebooting
5 Synthetic phages for substance redevelop
5.1 Chemical engineering cue in synthetic phage
5.2 Physical science cue control by synthetic phage
5.3 Multifunctional phage materials
6 Synthetic phage library
6.1 Reconstruction of synthetic phage
6.2 Genetic engineering of synthetic phage
7 Production synthetic phage parts from phage genomes
7.1 Phage RNA polymerases
7.2 Transcriptional regulators
7.3 Integrases
8 Application
8.1 Vaccine
8.2 Phage therapy (PT)
8.3 Selected protists Cells
8.4 Phage display
8.5 Nanomaterials
8.6 Phages developed through genetic engineering to promote tissue formation
8.7 Biofilms
8.8 Phage-based affection
8.9 Phage in the diagnostic of cancer
8.10 Multidrug resistant
8.11 Phage-based drug delivery
8.12 Phage as anti-infective
9 Conflicts in phage remedy of synthetic phage
10 Conclusions
11 Future with synthetic phage
References
Chapter Four: Phage engineering for development of diagnostic tools
1 Introduction.
3.1.4 Phage LUZ242s Igy peptide and DNA gyrase
3.1.5 Summary table
3.2 Interactions in Gram-positive bacteria
4 Phage lysis of the host
5 Modeling for phage therapy
5.1 In vitro cell line models
5.1.1 Cytotoxicity and pro-inflammatory characteristics
5.1.2 Mechanisms of interaction with tissues
5.2 Animal models
5.2.1 Invertebrate models
5.2.2 Vertebrate models
5.2.3 Immunogenicity and toxicity in animal models
5.3 Clinical trials in humans
6 Conclusions
References
Chapter Seven: Phage and phage cocktails formulationsPhage and phage cocktails formulations
1 Introduction
2 Formulation of phage cocktails
3 Applications of phage cocktails
3.1 Application of phages in oral infections
3.2 Application of phages in urinary tract infections
3.3 Application of phages in biofilms treatment
4 Conclusion and future perspectives
Conflict of interest
References
Chapter Eight: Phages for treatment of Escherichia coli infectionsPhages for treatment of Escherichia coli infections
1 Introduction
2 T4-related phages for E. coli therapy
2.1 T4-related viruses infecting pathogenic E. coli strains
2.2 Characterisation of T4-related viruses for phage therapy
2.3 Isolation of bacteriophages from environmental sources
2.4 Large-scale phage preparation
2.5 Pharmaceutical formulation for phages
2.6 Evaluation of T4-related bacteriophage preparations in animal models
2.7 Case reports and clinical trials
2.8 Phage therapy against pathogenic E. coli: current state and outlook for the future.
3 Phage therapy of E. coli O157:H7
3.1 Introduction to E. coli O157:H7
3.2 General considerations for phages against E. coli O157:H7
3.3 Bacteriophages in food processing
3.4 Phage therapy in animals affected by E. coli O157:H7
4 Phage therapy
HGT implications.
5 Conclusions
References
Chapter Nine: Phages for treatment of Klebsiella pneumoniae infections Phages for treatment of Klebsiella pneumoniae infections
1 Introduction
2 Know your enemy: K. pneumoniae
3 Limitations of antibiotic therapy for K. pneumoniae infections
4 Phage therapy of K. pneumoniae
4.1 Isolation of K. pneumoniae phages
4.2 Morphotypes and taxonomy K. pneumoniae phages
4.3 Forms of therapeutic K. pneumoniae phages
4.4 In vitro characterization of K. pneumoniae phages
4.5 In vivo experiments of phage therapy against K. pneumoniae
4.6 Clinical studies on cases treated with Klebsiella phages
4.7 Challenges in phage therapy for K. pneumoniae
5 Conclusion
References
Chapter Ten: Phages for treatment of Salmonella spp infection
1 Introduction
2 Conventional treatment vs phage therapy
3 Rapid screening and detection methods associated with bacteriophage for Salmonella
4 Phages as a tool against Salmonella infections
4.1 Salmonella phages
4.2 Commercially available phage products and regulatory status
4.3 Salmonella endolysins
5 Challenges in using phages
6 Future prospects
7 Conclusion
Acknowledgments
References
Chapter Eleven: Phages for treatment of Staphylococcus aureus infection
1 Introduction
2 Identification, morphology and genomic characteristics of phages infecting S. aureus
2.1 Organization of S. aureus phages genomes
2.2 Structure and genome of phage K
2.3 Novel Staphylococcal phages with unique morphology and broad host range
2.3.1 Phage JD219
2.3.2 Phages V1SA19, V1SA20, and V1SA22
3 Phage cocktail development against S. aureus
3.1 APTC-C-SA01: A novel bacteriophage cocktail for S. aureus and MRSA Biofilms
3.2 Phage cocktail AB-SA01 targeting planktonic and biofilm cultures of S. aureus.

Phage Therapy. Part A /

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