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Targeted protein degradation /
| Call Number: | Libro Electrónico |
|---|---|
| Other Authors: | |
| Format: | Electronic eBook |
| Language: | Inglés |
| Published: |
Cambridge :
Academic Press,
2023.
|
| Series: | Methods in enzymology ;
v. 681. |
| Subjects: | |
| Online Access: | Texto completo |
Table of Contents:
- One - High-efficiency knock-in of degradable tags (dTAG) at endogenous loci in cell lines
- Two - High throughput E3 ligase degron binding assays for novel PROTAC ligand discovery
- Three - Synthesis of SNIPERs against BCR-ABL with kinase inhibitors and a method to evaluate their growth inhibitory activity derived from BCR-ABL degradation
- Four - Harnessing nanobodies for target protein degradation through the Affinity-directed PROtein Missile (AdPROM) system
- Five - Monitoring PROTAC interactions in biochemical assays using Lumit immunoassays
- Six - The In-Cell Western immunofluorescence assay to monitor PROTAC mediated protein degradation
- Seven - Cereblon target validation using a covalent inhibitor of neosubstrate recruitment
- Eight - Advancing targeted protein degrader discovery by measuring cereblon engagement in cells
- Nine - Quantitative measurement of PROTAC intracellular accumulation
- Ten - The importance of controls in targeted protein degradation: Determining mechanism
- Eleven - Crystallization of VHL-based PROTAC-induced ternary complexes
- Twelve - Targeted protein degradation through light-activated E3 ligase recruitment
- Thirteen - A heterobifunctional molecule system for targeted protein acetylation in cells