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|a 1368128499
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|a 9780323992657
|q (electronic bk.)
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|a 572/.636
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|a Integrated methods in protein biochemistry.
|n Part B /
|c edited by Arun K. Shukla.
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250 |
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|a First edition.
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264 |
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1 |
|a Cambridge, MA :
|b Academic Press,
|c 2023.
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|a 1 online resource :
|b illustrations (chiefly color)
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|a text
|b txt
|2 rdacontent
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|a computer
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|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in enzymology ;
|v 679
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|a Includes bibliographical references.
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|a Biochemical analysis of protein-protein interfaces underlying the regulation of bacterial secretion systems / Safoura Salar and Florian D. Schubot -- Quantitative structural proteomics in living cells by covalent protein painting / Ahrum Son, Sandra Pankow, Tom Casimir Bamberger, and John R. Yates III -- Probing protein misfolding and dissociation with an infrared free-electron laser / Hisashi Okumura, Takayasu Kawasaki, and Kazuhiro Nakamura -- Optimized protocols for the characterization of Cas12a activities / Lindsie Martin, Saadi Rostami, and Rakhi Rajan -- Proximity labeling and proteomics : Get to know neighbors / Norihiro Kotani, Tomoyuki Araki, Arisa Miyagawa-Yamaguchi, Tomoko Amimoto, Miyako Nakano, and Koichi Honke -- Looking at LPMO reactions through the lens of the HRP/Amplex Red assay / Anton A. Stepnov and Vincent G.H. Eijsink -- Bioinformatic prediction and experimental validation of RiPP recognition elements / Kyle E. Shelton and Douglas A. Mitchell -- The preparation of recombinant arginyltransferase 1 (ATE1) for biophysical characterization / Misti Cartwright, Verna Van, and Aaron T. Smith -- Testing anti-cancer drugs with holographic incoherent-light-source quantitative phase imaging / Daniel Zicha and Radim Chmelik -- A streamlined process for discovery and characterization of inhibitors against phenylalanyl-tRNA synthetase of Mycobacterium tuberculosis / Heng Wang and Shawn Chen -- Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry / Fan Yang and Chu Wang -- Generation of nucleotide-linked resins for identification of novel binding proteins / Shikha S. Chauhan and Emily E. Weinert -- Functional assay of light-induced ion-transport by rhodopsins / Shoko Hososhima, Rei Abe-Yoshizumi, and Hideki Kandori -- Assay design for analysis of human uracil DNA glycosylase / Rashmi S. Kulkarni, Sharon N. Greenwood, and Brian P. Weiser -- Measurement of kinetic isotope effects on peptide hydroxylation using MALDI-MS / Michael A. Mingroni, Vanessa Chaplin Momaney, Alexandra N. Barlow, Isabella Jaen Maisonet, and Michael J. Knapp -- Continuous photometric activity assays for lytic polysaccharide monooxygenase--Critical assessment and practical considerations / Lorenz Schwaiger, Alice Zenone, Florian Csarman, and Roland Ludwig.
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|a Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein �? subunit and PLC-� enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more. Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology series Includes the latest information on Integrated Methods in Protein Biochemistry.
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|a Proteins
|x Analysis
|x Research
|x Methodology.
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650 |
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|a Proteomics
|x Methodology.
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|a Biochemistry.
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6 |
|a Prot�eines
|0 (CaQQLa)201-0031951
|x Analyse
|0 (CaQQLa)201-0031951
|x Recherche
|0 (CaQQLa)201-0380850
|x M�ethodologie.
|0 (CaQQLa)201-0380850
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650 |
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6 |
|a Prot�eomique
|0 (CaQQLa)201-0363192
|x M�ethodologie.
|0 (CaQQLa)201-0379663
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650 |
|
6 |
|a Biochimie.
|0 (CaQQLa)201-0001077
|
650 |
|
7 |
|a biochemistry.
|2 aat
|0 (CStmoGRI)aat300054538
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650 |
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7 |
|a Biochemistry
|2 fast
|0 (OCoLC)fst00831961
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700 |
1 |
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|a Shukla, Arun K.,
|e editor.
|
776 |
0 |
8 |
|c Original
|z 0323992641
|z 9780323992640
|w (OCoLC)1329421930
|
830 |
|
0 |
|a Methods in enzymology ;
|v v. 679.
|
856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/679
|z Texto completo
|