Three phase partitioning : applications in separation and purification of biological molecules and natural products /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Gupta, Munishwar Nath, Roy, Ipsita
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam : Elsevier, 2021.
Temas:
Phase partition.
Biomolecules > Separation.
Partage des phases.
Biomol�ecules > S�eparation.
Biomolecules > Separation
Phase partition
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front cover
  • Half title
  • Full title
  • Copyright
  • Contents
  • Contributors
  • Preface
  • The journey in understanding interactions of salts and solvents with proteins continues!
  • Chapter 1
  • Three phase partitioning: some reminiscences, some science
  • 1.1 The origin of the tpp method
  • 1.2 How does TPP work?
  • 1.3 Inhibition of enzyme activity by t-BuOH
  • 1.4 Enhanced activity
  • 1.5 Other molecules
  • 1.6 TPP doesn't work in all cases
  • References
  • Chapter 2
  • How and why we happen to use three phase partitioning in areas other than protein purification
  • 2.1 TPP for purification of proteins/enzymes
  • 2.2 TPP used for edible oil extraction
  • 2.3 TPP of polysaccharides
  • 2.4 TPP of microbial cells
  • 2.5 Isolation and purification of low molecular weight compounds
  • 2.6 Conclusion
  • References
  • Chapter 3
  • Fundamental aspects of protein isolation and purification
  • 3.1 Introduction
  • 3.2 Fusion tags and protein solubility
  • 3.3 Cell lysis
  • 3.3.1 Mechanical procedures
  • 3.4 Non-mechanical procedures
  • 3.4.1 Physical methods
  • 3.4.2 Chemical methods
  • 3.5 Protein precipitation
  • 3.5.1 Salting-in and salting out
  • 3.5.2 Trichloroacetic acid (TCA) and other procedures
  • 3.5.3 Protein precipitation by organic solvents
  • 3.5.4 Metal-chelate protein precipitation
  • 3.6 Affinity precipitation and immunoprecipitation
  • 3.7 Protein purification
  • 3.7.1 Dialysis
  • 3.7.2 Size exclusion chromatography (SEC)
  • 3.7.3 Ion exchange (IEC) chromatography
  • 3.7.4 Hydrophobic chromatography/ hydrophobic interaction chromatography (HIC)
  • 3.7.5 Affinity interaction chromatography (AIC)
  • 3.7.6 Metal chelate chromatography
  • 3.8 Conclusions and outlooks
  • References.
  • Chapter 4
  • The multiple facets of three-phase partitioning in the purification, concentration, yield and activity of enzy ...
  • 4.1 Introduction
  • 4.2 TPP as a rapid single step procedure to isolate and concentrate proteins
  • 4.3 TPP concentrates proteins by decreasing the volume of water
  • 4.4 TPP concentrates individual proteins by removing unwanted proteins
  • 4.5 TPP preserves and increases enzyme activity
  • 4.6 Explanations for TPP preserving and increasing enzyme activity
  • 4.7 Purification and/or refolding of denatured enzymes with TPP
  • 4.8 TPP purification of recombinant HIS-Tag fusion proteins or metal binding proteins
  • 4.9 TPP purification of proteins with affinity ligands
  • 4.10 Ultrasound assisted TPP to isolate proteins, oils and polysaccharides
  • 4.11 Microwave assisted TPP
  • 4.12 Versatility of TPP. separating DNA, carbohydrates and oils and two-step TPP protocols
  • 4.13 Conclusion
  • References
  • Chapter 5
  • Enzymes recovery by three phase partitioning
  • 5.1 Introduction
  • 5.2 Overview of the studies and conditions of use of three phase partitioning and its variants for the recovery of enzymes
  • 5.3 Three phase partitioning for the recovery of glycosidases
  • 5.4 Three phase partitioning for the recovery of proteases
  • 5.5 Three phase partitioning for the recovery of oxidoreductases
  • 5.6 Three phase partitioning for the recovery of lipases
  • 5.7 Three phase partitioning for the recovery of other hydrolases
  • 5.8 Conclusion
  • References
  • Chapter 6
  • Emulsion gel formation in three phase partitioning
  • 6.1 Introduction
  • 6.2 The mechanism of TPP
  • 6.2.1 The effect of ammonium sulfate and tert -butanol
  • 6.2.2 Protein adsorption at the interface
  • 6.2.3 The shear rheological properties of interfacial protein layer.
  • 6.2.4 The role of emulsion stability in the formation of gel as the middle phase
  • 6.3 Conclusion
  • References
  • Chapter 7
  • Three-phase partitioning (TPP) of proteases from parasites, plants, tissue and bacteria for enhanced activity
  • 7.1 Why we are interested in proteases
  • 7.2 Three-phase partitioning as a protease purification tool
  • 7.3 Conditions considered during optimization of tpp
  • 7.3.1 Choice of organic solvent and its ratio to crude extract
  • 7.3.2 Choice of salt and concentration of salt used for tpp
  • 7.3.3 Effect of pH and pI on tpp
  • 7.3.4 Effect of temperature on tpp
  • 7.3.5 Parasite proteases as drug and diagnostic targets
  • 7.3.6 Plant proteases for milk-clotting in cheese making
  • 7.3.7 Bacterial proteases for laundry detergents and anti-inflammatory agents
  • 7.3.8 Fish and bacterial proteases in food biotechnology
  • 7.3.9 Protease inhibitors
  • 7.4 Effect of tpp on protease structure and activity
  • 7.5 Conclusions
  • References
  • Chapter 8
  • Three phase partitioning of plant peroxidases
  • 8.1 Peroxidases
  • 8.2 Sources and functions of peroxidases
  • 8.2.1 Mammalian peroxidases
  • 8.2.2 Microbial peroxidases
  • 8.2.3 Plant peroxidases
  • 8.3 Biotechnological applications of plant peroxidases
  • 8.3.1 Pulp and paper industry
  • 8.3.2 Bioremediation of phenolic compounds
  • 8.3.3 Decolourization of industrial dyes
  • 8.3.4 Biosensor
  • 8.3.5 Analysis and diagnostic kits
  • 8.3.6 Hair dyeing
  • 8.4 Three phase partitioning system
  • 8.4.1 Effect of salt on TPP
  • 8.4.2 Effect of t-butanol on TPP
  • 8.4.3 Effect of temperature on TPP
  • 8.4.4 Effect of pH on TPP
  • 8.5 TPP as an emerging technique for plant peroxidase purification
  • 8.6 Conclusion
  • References.
  • Chapter 9
  • Macro-(affinity ligand) facilitated three phase partitioning Converting TPP into an affinity based process
  • 9.1 Introduction
  • 9.1.1 Importance of affinity interactions in protein purification
  • 9.2 Water soluble polymers and smart polymers
  • 9.2.1 Carrageenans
  • 9.2.2 Alginates
  • 9.2.3 Chitosan
  • 9.2.4 Eudragits
  • 9.3 Smart biocatalysts
  • 9.4 MLFTPP
  • 9.5 Conclusion/future perspectives
  • References
  • Chapter 10
  • Applications of three phase partitioning and macro-(affinity ligand) facilitated three phase partitioning in ...
  • 10.1 Introduction
  • 10.1.1 Protein structure
  • 10.1.2 General strategies for protein refolding
  • 10.1.3 Smart polymers and protein refolding
  • 10.1.4 Refolding by TPP
  • 10.1.5 Refolding by MLFTPP
  • 10.1.6 Structural changes in proteins due to TPP
  • 10.1.7 Some other underexploited applications of TPP treatment of proteins/cells
  • 10.2 Conclusion
  • References
  • Chapter 11
  • Three phase partitioning-based strategies for highly efficient separation of bioactive polysaccharides from n ...
  • 11.1 Introduction
  • 11.2 Factors affecting the TPP process for extraction of PSs
  • 11.2.1 Ammonium sulfate
  • 11.2.2 t -butanol
  • 11.2.3 pH
  • 11.2.4 Temperature
  • 11.2.5 Time
  • 11.3 Process intensification of TPP system for PSs extraction
  • 11.3.1 Enzyme-assisted TPP
  • 11.3.2 US-assisted TPP
  • 11.3.3 US-synergized TPP
  • 11.4 TPP combined with downstream techniques
  • 11.5 TPP separation influencing the properties of PSs
  • 11.6 Conclusions
  • Acknowledgements
  • References
  • Chapter 12
  • Technologies for oil extraction from oilseeds and oleaginous microbes
  • 12.1 Introduction
  • 12.1.1 Oilseeds and nutritional security
  • 12.2 Importance of oil and lipid extraction
  • 12.3 Green solvents and techniques for oil and lipid extraction.
  • 12.3.1 Selection of extraction solvent
  • 12.4 Green solvents for oil/ lipid extraction
  • 12.4.1 Bio-derived solvents
  • 12.4.2 Supercritical fluid technology
  • 12.4.3 Ionic liquids and deep eutectic solvents
  • 12.4.4 Switchable solvents
  • 12.5 Conventional and green extraction techniques for oil/ lipid extraction
  • 12.5.1 Oil/Expeller pressing
  • 12.5.2 Bead milling
  • 12.5.3 Enzyme assisted oil/lipid extraction from oilseeds/biomass
  • 12.5.4 Microwave treatment
  • 12.5.5 Ultrasound assisted extraction (UAE)
  • 12.6 Conclusion
  • Author's contributions
  • Competing interest
  • Acknowledgements
  • References
  • Chapter 13
  • Three phase partitioning (TPP) as an extraction technique for oleaginous materials
  • 13.1 Introduction
  • 13.2 Conventional extraction techniques for oleaginous material
  • 13.3 Mechanism of extraction using TPP
  • 13.4 Advantages of TPP
  • 13.5 Factors affecting TPP
  • 13.5.1 Salt
  • 13.5.2 Extraction solvent
  • 13.5.3 pH
  • 13.5.4 Temperature
  • 13.5.5 Extraction time
  • 13.6 Hyphenated TPP-techniques
  • 13.6.1 Enzyme assisted TPP (EATPP)
  • 13.6.2 Ultrasound assisted TPP (UATPP)
  • 13.6.3 Microwave assisted TPP (MATPP)
  • 13.6.4 High-pressure homogenization assisted TPP (HPHTPP)
  • 13.7 Challenges and future perspectives
  • References
  • Chapter 14
  • Intensification of extraction of biomolecules using three-phase partitioning
  • 14.1 Introduction
  • 14.1.1 History
  • 14.1.2 TPP process and mechanism
  • 14.2 Key factors affecting the TPP method
  • 14.2.1 Salt concentration
  • 14.2.2 Crude extract to alcohol ratio
  • 14.2.3 pH
  • 14.2.4 Temperature
  • 14.3 Advanced TPP processes
  • 14.3.1 Two-step TPP
  • 14.3.2 Macro-affinity ligand-facilitated three-phase partitioning (MLFTPP)
  • 14.3.3 Ionic liquid three-phase partitioning (ILTPP).

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