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Ion channels : channel biochemistry, reconstitution, and function /
| Clasificación: | Libro Electrónico |
|---|---|
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge :
Academic Press,
2021.
|
| Colección: | Methods in enzymology ;
v. 652. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Section I. Structure/function relationships. Correlating ion channel structure and function / Philipp A.M. Schmidpeter and Crina M. Nimigean ; TRPV3 expression and purification for structure determination by Cryo-EM / Arthur Neuberger, Kirill D. Nadezhdin, and Alexander I. Sobolevsky ; Methods to study phosphoinositide regulation of ion channels / Yevgen Yudin, Luyu Liu, Janhavi Nagwekar, and Tibor Rohacs ; Recombinant expression and purification of pentameric ligand-gated ion channels for Cryo-EM structural studies / Arvind Kumar, Sandip Basak, and Sudha Chakrapani ; Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography / Andrea Saponaro, Atiyeh Sadat Sharifzadeh, and Anna Moroni
- Section II. Structural and chemical methods to assess channel dynamics. Structural dynamics of channels and transporters by high-speed atomic force microscopy / George R. Heath, Yi-Chih Lin, Tina R. Matin, and Simon Scheuring ; Crosslinking glutamate receptor ion channels / Andrew J.R. Plested and Mette H. Poulsen ; Single molecule FRET methodology for investigating glutamate receptors / Nabina Paudyal, Nidhi Kaur Bhatia, and Vasanthi Jayaraman ; Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues / Megumi Yamashita and Murali Prakriya
- Section III. Channel reconstitution and studies in bilayers. A quantitative flux assay for the study of reconstituted Cl⁻ channels and transporters / Eva Fortea and Alessio Accardi ; Ion channel reconstitution in lipid bilayers / Eleonora Zakharian ; Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers / Oliver Rauh, Kerri Kukovetz, Laura Winterstein, Bianca Introini, and Gerhard Thiel
- Section IV. Methods to assess oligomerization. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture / Randy B. Stockbridge.