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Enzyme engineering and evolution : general methods /

Bibliographic Details
Call Number:Libro Electrónico
Other Authors: Tawfik, Dan S. (Editor)
Format: Electronic eBook
Language:Inglés
Published: Cambridge, MA : Academic Press, 2020.
Edition:First edition.
Series:Methods in enzymology ; v. 643.
Subjects:
Online Access:Texto completo
Table of Contents:
  • In vivo site-directed recombination (SDR) : An efficient tool to reveal beneficial epistasis / Javier Vi�na-Gonzalez and Miguel Alcalde
  • A beginner's guide to molecular dynamics simulations and the identification of cross-correlation networks for enzyme engineering / Haoran Yu and Paul A. Dalby
  • Exploration of enzyme diversity : High-throughput techniques for protein production and microscale biochemical characterization / Michal Vasina, Pavel Vanacek, Jiri Damborsky, and Zbynek Prokop
  • Emulsion-based directed evolution of enzymes and proteins in yeast / Elizabeth C. Gardner, Ella J. Watkins, Jimmy Gollihar, and Andrew D. Ellington
  • Remodeling enzyme active sites by stepwise loop insertion / Md Anarul Hoque, Yong Zhang, Zhi Li, Li Cui, and Yan Feng
  • Consensus Finder web tool to predict stabilizing substitutions in proteins / Bryan J. Jones, Chi Nok Enoch Kan, Christine Luo, and Romas J. Kazlauskas
  • The use of consensus sequence information to engineer stability and activity in proteins / Matt Sternke, Katherine W. Tripp, and Doug Barrick
  • Structure-guided rational design of the substrate specificity and catalytic activity of an enzyme / Jung Min Choi and Hak-Sung Kim
  • Biological fitness landscapes by deep mutational scanning / Jacob D. Mehlhoff and Marc Ostermeier
  • Focused rational iterative site-specific mutagenesis (FRISM) / Danyang Li, Qi Wu, and Manfred T. Reetz
  • Statistical analysis of mutational epistasis to reveal intramolecular interaction networks in proteins / Charlotte M. Miton, John Z. Chen, Kalum Ost, Dave W. Anderson, and Nobuhiko Tokuriki
  • Machine learning-assisted enzyme engineering / Niklas E. Siedhoff, Ulrich Schwaneberg, and Mehdi D. Davari
  • Ultrahigh throughput screening for enzyme function in droplets / Stefanie Neun, Paul J. Zurek, Tomasz S. Kaminski, and Florian Hollfelder.