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200629s2020 maua ob 000 0 eng d |
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|a YDX
|b eng
|e rda
|e pn
|c YDX
|d OPELS
|d YDXIT
|d OCLCO
|d UKMGB
|d EBLCP
|d OCLCF
|d OCLCA
|d OCLCO
|d OCLCQ
|d COM
|d OCLCO
|d OCLCQ
|d CASUM
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|a GBC079341
|2 bnb
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|a 019825867
|2 Uk
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|a 1163354421
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|a 9780128215326
|q (electronic book)
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|a 0128215321
|q (electronic book)
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|z 9780128215319
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|z 0128215313
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|a (OCoLC)1160065540
|z (OCoLC)1163354421
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|a QH588.S83
|b H86 2020
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|a QU 325
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| 082 |
0 |
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|a 616.02/774
|2 23
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| 245 |
0 |
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|a Human pluripotent stem cell derived organoid models /
|c edited by Jason R. Spence.
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| 264 |
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1 |
|a Cambridge, MA :
|b Academic Press,
|c 2020.
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| 300 |
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|a 1 online resource (xiv, 321 pages) :
|b illustrations (chiefly color)
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in cell biology ;
|v volume 159
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| 504 |
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|a Includes bibliographical references.
|
| 588 |
0 |
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|a Online resource; title from digital title page (viewed on July 15, 2020).
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0 |
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|a Intro -- Human Pluripotent Stem Cell Derived Organoid Models -- Copyright -- Contents -- Contributors -- Chapter 1: Generation of esophageal organoids and organotypic raft cultures from human pluripotent stem cells -- 1. Introduction -- 2. Significance and applications -- 3. Morphological and transcriptional analysis of developing esophageal cultures -- 4. Overview of differentiation protocol -- 5. Step-by-step protocol -- 5.1. Coating plates for stem cell culture maintenance and directed differentiation -- 5.1.1. Materials and reagents -- 5.1.2. Protocol
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| 505 |
8 |
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|a 5.2. Differentiation of stem cells into esophageal organoids -- 5.2.1. Materials, reagents and equipment -- 5.2.2. Solutions preparation -- 5.2.3. Protocol (Fig. 2A) -- 5.2.3.1. hPSC dissociation and plating in 24-well plate (day-1) -- 5.2.3.2. Differentiation protocol -- 5.2.3.3. Embedding foregut spheroids in Matrigel -- 5.2.3.4. Reduction of HEOs density -- 5.3. Esophageal raft culture protocol -- 5.3.1. Materials and reagents -- 5.3.2. Coating 100mm plates with collagen IV -- 5.3.3. Dissociation of HEOs and culturing in keratinocyte media
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| 505 |
8 |
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|a 5.3.4. Preparing collagen-Mouse fibroblasts gels for raft cultures -- 5.3.5. Transferring cells from keratinocyte media to raft cultures -- 5.4. Immunofluorescence analysis of HEOs and organotypic raft cultures (Fig. 4) -- 5.4.1. Materials and reagents -- 5.4.2. Tissue fixation, preparation and cryosection -- 5.4.3. Immunofluorescent protocol -- 6. Future directions -- 7. Summary -- Acknowledgments -- References -- Chapter 2: Generation and use of gastric organoids for the study of Helicobacter pylori pathogenesis -- 1. Introduction -- 2. Significance and applications of gastric organoids
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| 505 |
8 |
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|a 3. Overview of the protocol -- 4. Step-by-step protocol -- 4.1. Generation of human-derived gastric organoids from normal and tumor tissues -- 4.1.1. Materials and reagents -- 4.1.2. Procedure: Generation of human-derived normal gastric organoids -- 4.1.3. Procedure: Generation of human-derived tumor gastric organoids -- 4.2. Generation of human-PBMC derived immune cells -- 4.2.1. Materials and reagents -- 4.2.2. Procedure: Isolation of PBMCs from whole blood using Ficoll-Paque density gradient medium -- 4.2.3. Procedure: Isolation of PBMCs from whole blood using Lymphoprep
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| 505 |
8 |
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|a 4.2.4. Procedure: Freezing of PBMCs -- 4.2.5. Procedure: Culture of dendritic cells (DCs) -- 4.2.6. Procedure: Culture of cytotoxic T lymphocytes (CTLs) -- 4.2.7. Procedure: Culture of myeloid-derived suppressor cells (MDSCs) -- 4.3. Organoid/immune cell co-culture -- 4.3.1. Procedure: DCs and CTL co-culture -- 4.3.2. Procedure: Organoid-immune cell co-culture -- 4.4. Orthotopic transplantation -- 4.4.1. Materials and reagents -- 4.4.2. Procedure: Orthotopic transplantation of human-derived gastric organoids -- 5. Precursor techniques -- 6. Safety considerations and standards
|
| 650 |
|
0 |
|a Stem cells.
|
| 650 |
|
2 |
|a Stem Cells
|0 (DNLM)D013234
|
| 650 |
|
6 |
|a Cellules souches.
|0 (CaQQLa)201-0058821
|
| 650 |
|
7 |
|a Stem cells
|2 fast
|0 (OCoLC)fst01132975
|
| 655 |
|
4 |
|a Internet Resources.
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| 655 |
|
4 |
|a Index not Present.
|
| 700 |
1 |
|
|a Spence, Jason R.
|q (Jason Robert),
|d 1977-
|e editor.
|
| 776 |
0 |
8 |
|i Print version:
|t Human pluripotent stem cell derived organoid models.
|d Cambridge, MA : Academic Press, 2020
|z 0128215313
|z 9780128215319
|w (OCoLC)1133125669
|
| 830 |
|
0 |
|a Methods in cell biology ;
|v v. 159.
|
| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/159
|z Texto completo
|
