Molecular biology techniques : a classroom laboratory manual /

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Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergra...

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Bibliographic Details
Call Number:Libro Electrónico
Main Authors: Carson, Sue (Susan) (Author), Miller, Heather B. (Author), Srougi, Melissa C. (Author), Witherow, D. Scott (Author)
Format: Electronic eBook
Language:Inglés
Published: London, United Kingdom : Academic Press, an imprint of Elsevier, 2019.
Edition:Fourth edition.
Subjects:
Molecular biology > Laboratory manuals.
Genetics > Technique.
Genetic Techniques
Molecular Biology > methods
CRISPR-Cas Systems
Biologie mol�eculaire > Manuels de laboratoire.
G�en�etique > Technique.
Syst�emes CRISPR-Cas.
Molecular biology.
Laboratory Manual
Laboratory manuals.
Manuels de laboratoire.
Online Access:Texto completo
Table of Contents:
  • Part I. Manipulation of DNA
  • Getting oriented: practicing with micropipettes
  • Purification and digestion of plasmid (vector) DNA
  • Completion of vector preparation and polymerase chain reaction amplification of egfp
  • Preparation on insert DNA (egfp) PCR product
  • DNA ligation and transformation of Escherichia coli
  • Part II. Screening transformants
  • Screening of transformants, part I
  • Screening of transformants, part II
  • Analysis of DNA sequence from a positive clone
  • Part III. Expression, detection, and purification of recombinant proteins from bacteria
  • Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part I
  • Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part II
  • Extraction of recombinant protein from Escherichia coli using a glutathione affinity culture
  • Analysis of purification fractions
  • Part IV. Analysis of mRNA levels
  • Total RNA purification
  • Analysis of gst:egfp mRNA levels by RT-qPCR: Part I
  • Analysis of gst:egfp mRNA levels by RT-qPCR: Part II
  • Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part I
  • Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part II
  • Part V. Modulation of gene expression
  • Culturing mammalian cells
  • Transient transfection of mammalian cells
  • RNAi-mediated knockdown of EGFP, Part I
  • RNAi-mediated knockdown of EGFP, Part II
  • RNAi-mediated knockdown of EGFP, Part III
  • CRISPR-mediated knockout of EGFP, Part I
  • CRISPR-mediated knockout of EGFP, Part II
  • Advanced CRISPR, Part I
  • Advanced CRISPR, Part II
  • Interim laboratory session
  • Advanced CRISPR, Part III
  • Advanced CRISPR, Part IV
  • Advanced CRISPR, Part V
  • Advanced CRISPR, Part VI.

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