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Enzymes as sensors /

Enzymes as Sensors, Volume 589 in the Methods in Enzymology series, covers a variety of topics, including advances in genetically coded fluorescent sensors, enzymes as sensors, and bioapplications of electrochemical sensors and biosensors. Users will find a comprehensive discussion of timely topics...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Thompson, Richard B. (Editor ), Fierke, Carol A. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cambridge, MA : Academic Press, 2017.
Colección:Methods in enzymology ; v. 589.
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Recent advances in development of genetically encoded fluorescent sensors
  • Engineering rugged field assays to detect hazardous chemicals using spore-based bacterial biosensors
  • Engineering BRET-sensor proteins
  • Enzymes as sensors
  • Integrated strategies to gain a systems-level view of dynamic signaling networks
  • Probing Cdc42 polarization dynamics in budding yeast using a biosensor
  • Novel fluorescence-based biosensors incorporating unnatural amino acids
  • Folding- and dynamics-based electrochemical DNA sensors
  • Construction of protein-based biosensors using ligand-directed chemistry for detecting analyte binding
  • Measuring and imaging metal ions with fluorescence-based biosensors: speciation, selectivity, kinetics, and other issues
  • Bioapplications of electrochemical sensors and biosensors
  • A highly sensitive biosensor for ATP using a chimeric firefly luciferase
  • Highly modular bioluminescent sensors for small molecules and proteins
  • Sensitive protein detection and quantification in paper-based microfluidics for the point of care
  • Microneedle enzyme sensor arrays for continuous in vivo monitoring
  • Visualization of the genomic loci that are bound by specific multiprotein complexes by biomolecular fluorescence complementation analysis on Drosophila polytene chromosomes
  • Author index
  • Subject index.