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|a Molecular characterization of autophagic responses.
|n Part B /
|c edited by Lorenzo Galluzzi, Jos�e Manuel Bravo-San Pedro and Guido Kroemer.
|
| 264 |
|
1 |
|a Cambridge, MA :
|b Academic Press is an imprint of Elsevier,
|c 2017.
|
| 300 |
|
|
|a 1 online resource
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
|
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 347 |
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|a data file
|2 rda
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| 490 |
1 |
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|a Methods in enzymology ;
|v volume 588
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| 504 |
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|a Includes bibliographical references.
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| 505 |
0 |
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|a Front Cover; Molecular Characterization of Autophagic Responses, Part B; Copyright; Contents; Contributors; Preface; 1. Introduction; Acknowledgments; References; Chapter One: Renilla Luciferase-LC3 Based Reporter Assay for Measuring Autophagic Flux; 1. Introduction; 1.1. The Challenge of Measuring Autophagic Flux; 1.2. LC3 as a Marker of Autophagy; 1.3. Cautions in Using LC3-II Turnover as a Marker of Autophagic Flux; 2. The Rluc-LC3 Assay; 2.1. Description of the Assay; 2.2. Applications of the Rluc-LC3 Assay; 3. Designing the Rluc-LC3wt and Rluc-LC3G120A Fusion Proteins
|
| 505 |
8 |
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|a 4. Establishing Rluc-LC3wt- and Rluc-LC3G120A-Expressing Cells4.1. Expression Vectors and Cell Lines; 4.1.1. Stable Transfection of MCF7 Cells (Transfections Are Performed Mainly According to the Fugene HD Protocol (Promega ... ; 5. The Rluc-LC3 Assay Performed on Cell Lysates; 5.1. Buffers and Solutions; 5.2. Protocol; 6. The Rluc-LC3 Assay Performed on Live Cells; 6.1. Buffers and Solutions; 6.2. Protocol; 6.3. Notes; Acknowledgments; References; Chapter Two: Measurement of Autolysosomal pH by Dual-Wavelength Ratio Imaging; 1. Introduction; 2. Cell Preparation
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| 505 |
8 |
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|a 3. Equipment Setup and Software Requirements4. Image Acquisition; 5. In Situ pH Calibration; 6. Image Analysis and Determination of Autolysosomal pH; 7. Conclusion; Acknowledgments; References; Chapter Three: Long-Lived Protein Degradation During Autophagy; 1. Introduction; 2. Proteolysis; 2.1. Materials and Reagents; 2.2. Protocol (see Fig. 1); 2.3. Notes and Potential Pitfalls; 3. Other Methods for Measuring Autophagic Flux; 4. Conclusion; Acknowledgments; References
|
| 505 |
8 |
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|a Chapter Four: Proteomic Profiling of De Novo Protein Synthesis in Starvation-Induced Autophagy Using Bioorthogonal Noncan ... 1. Introduction; 2. Materials, Equipment, and Solutions; 2.1. Materials; 2.2. Equipment; 2.3. Methionine-Free DMEM; 2.4. Amino Acid-Free Medium; 2.5. C18 Buffers; 2.6. Gradient Separation Buffer; 3. Fundamentals: AHA Labeling Combined With the iTRAQ Approach for Identification of De Novo Protein Synthesis; 3.1. General Principles; 3.2. Advantages of This Approach; 3.3. Applications of This Approach; 4. Protocol; 4.1. Cell Culture and Metabolic Labeling by AHA
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| 505 |
8 |
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|a 4.2. Click Chemistry Tagging With Biotin Alkyne4.3. Avidin Affinity Purification; 4.4. Isobaric Tag for Relative and Absolute Quantification (iTRAQ) Labeling; 4.5. Sample Cleanup by Strong Cation Exchange Chromatography; 4.6. Desalting of Labeled Samples by the C18 Column; 4.7. Nano-LC Electrospray Ionization MS; 4.8. Protein Identification and Quantification Using ProteinPilotTM Software; 5. Limitations; References; Chapter Five: Methods to Monitor and Manipulate TFEB Activity During Autophagy; 1. Introduction; 2. Methods to Monitor TFEB/TFE3 Activation
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| 588 |
0 |
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|a Online resource; title from PDF title page (ScienceDirect, viewed March 6, 2017).
|
| 650 |
|
0 |
|a Cytology.
|
| 650 |
|
0 |
|a Autophagic vacuoles.
|
| 650 |
|
0 |
|a Cell death.
|
| 650 |
|
2 |
|a Autophagy
|0 (DNLM)D001343
|
| 650 |
|
2 |
|a Cell Biology
|0 (DNLM)D003585
|
| 650 |
|
2 |
|a Cytology
|0 (DNLM)D000093222
|
| 650 |
|
6 |
|a Cytologie.
|0 (CaQQLa)201-0040099
|
| 650 |
|
6 |
|a Cellules
|x Mort.
|0 (CaQQLa)201-0110794
|
| 650 |
|
6 |
|a Autophagie (Cytologie)
|0 (CaQQLa)201-0273011
|
| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Anatomy & Physiology.
|2 bisacsh
|
| 650 |
|
7 |
|a Autophagic vacuoles
|2 fast
|0 (OCoLC)fst00824188
|
| 650 |
|
7 |
|a Cell death
|2 fast
|0 (OCoLC)fst00850180
|
| 650 |
|
7 |
|a Cytology
|2 fast
|0 (OCoLC)fst00886282
|
| 700 |
1 |
|
|a Galluzzi, Lorenzo,
|e editor.
|
| 700 |
1 |
|
|a Kroemer, Guido,
|e editor.
|
| 700 |
1 |
|
|a Bravo-San Pedro, Jose Manuel,
|e editor.
|
| 776 |
0 |
8 |
|i Print version:
|t Molecular characterization of autophagic responses. Part B.
|d Cambridge, MA : Academic Press is an imprint of Elsevier, 2017
|z 0128096748
|z 9780128096741
|w (OCoLC)959885659
|
| 830 |
|
0 |
|a Methods in enzymology ;
|v v. 588.
|
| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/588
|z Texto completo
|
