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Proteomics in Biology. Part A /
Proteomics in Biology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in proteomics.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge, MA :
Academic Press is an imprint of Elsevier,
2017.
|
| Colección: | Methods in enzymology ;
v. 585. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Proteomics in Biology. |n Part A / |c edited by Arun K. Shukla. |
| 264 | 1 | |a Cambridge, MA : |b Academic Press is an imprint of Elsevier, |c 2017. | |
| 300 | |a 1 online resource (xviii, 529 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 490 | 1 | |a Methods in enzymology ; |v volume 585 | |
| 546 | |a Text in English. | ||
| 505 | 0 | |a Front Cover; Proteomics in Biology, Part A; Copyright; Contents; Contributors; Preface; Chapter One: An Easy and Fast Protocol for Affinity Bead-Based Protein Enrichment and Storage of Proteome Samples; 1. Introduction; 2. StrataClean Beads for Protein Enrichment; 2.1. A General Overview of the Method; 3. General Protocol for the Use of StrataClean Beads in Proteomics; 3.1. Priming and Washing of the Beads; 3.2. Affinity Binding of Proteins in Solution; 3.3. Elution of the Purified Proteins; 4. Application of StrataClean Beads in Protein Sample Preparation and Life Science Research. | |
| 505 | 8 | |a 4.1. Highly Diluted Protein Solutions; 4.2. Optimization and Monitoring of Biotechnological Processes/Screening of Biological Samples; 4.3. Storage and Shipping of Protein Samples; 5. Protocol Variations; 5.1. Storage of Primed Beads; 5.2. Duration of Bead Incubation; 5.3. Use of Buffers Containing Urea; 5.4. Influence of the Salt Concentration; 6. Conclusions; References; Chapter Two: Filter-Aided Sample Preparation: The Versatile and Efficient Method for Proteomic Analysis; 1. Introduction; 2. The FASP Procedures; 2.1. FASP Methods Overview; 2.2. Selection of the Ultrafiltration Device. | |
| 505 | 8 | |a 2.3. Limitations in Sample Size; 2.4. FASP Allows Consecutive Protein Digestion With Different Proteases; 2.5. Multipurpose Applications of FASP; 3. Filter-Aided Sample Preparation Protocols; 3.1. Preparation of Lysates from Tissues and Cells; 3.2. Determination of Total Protein in Lysates by WF-Assay Using Fluorescence Microtiter Plate Reader; 3.3. MED-FASP Protocol; 3.4. FASP Protocol; 3.5. TAPEG-FASP Protocol; 3.5.1. Synthesis of TAPEG (Optional); 3.5.2. Workflow; Acknowledgments; References; Chapter Three: An Overview of Advanced SILAC-Labeling Strategies for Quantitative Proteomics. | |
| 505 | 8 | |a 1. Introduction; 2. Dual Labeling to Exclude Unlabeled Proteins; 2.1. Cell-Cell Contact Analyses; 2.2. Secretion Analyses; 2.3. Analyses of Postmitotic Neurons; 2.4. Use of Nondialyzed Serum; 3. Subsaturating Labeling; 3.1. Increasing Measurement Accuracy for Medium to Slow Turnover Proteins; 3.2. Quadruple Subsaturating Labeling to Separately Measure Decay and Synthesis After Cellular Stimulation; 3.3. Determining the Contribution of Extra- vs Intracellular Amino Acids toProtein Synthesis; 4. 5-Plexed SILAC Labeling; 5. Conclusion; References. | |
| 505 | 8 | |a Chapter Four: Label-Free and Standard-Free Absolute Quantitative Proteomics Using the "Total Protein" and "Proteomic R ... 1. Introduction; 2. TPA: For Determination of Protein Contents and Concentrations; 3. Cell Size and Protein Copy Numbers Can Be Assessed by the Proteomic Ruler; 4. Protein Concentrations and Copy Numbers Provide Different Layers of Information; 5. Consistency of TPA Values With Biochemical Data; 5.1. DNA and RNA Content; 5.2. Enzymatic Activities; 5.3. Subcellular Fractionation; 6. Normalization and Comparison of Datasets Using DJ-1/PARK7 Titer; 7. Limitations. | |
| 500 | |a Includes indexes. | ||
| 588 | 0 | |a Online resource; title from title details screen (ScienceDirect, viewed January 30, 2017). | |
| 520 | |a Proteomics in Biology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in proteomics. | ||
| 504 | |a Includes bibliographical references at the end of each chapters and indexes. | ||
| 650 | 0 | |a Proteomics. | |
| 650 | 1 | 2 | |a Proteomics |x methods |0 (DNLM)D040901Q000379 |
| 650 | 2 | |a Proteomics |0 (DNLM)D040901 | |
| 650 | 6 | |a Prot�eomique. |0 (CaQQLa)201-0363192 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Proteomics |2 fast |0 (OCoLC)fst01079785 | |
| 700 | 1 | |a Shukla, Arun K., |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Proteomics in biology. Part A. |d Cambridge, MA : Academic Press is an imprint of Elsevier, 2017 |z 0128097426 |z 9780128097427 |w (OCoLC)959033467 |
| 830 | 0 | |a Methods in enzymology ; |v v. 585. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/585 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/journal/00766879/585 |z Texto completo |