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SCIDIR_ocn892069067 |
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OCoLC |
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20231120111803.0 |
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m o d |
| 007 |
cr cnu---unuuu |
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141003s1984 nyua o 000 0 eng d |
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|a OPELS
|b eng
|e rda
|e pn
|c OPELS
|d N$T
|d EBLCP
|d DEBSZ
|d YDXCP
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|d OCLCO
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|d OCLCO
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| 019 |
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|a 898422339
|a 948610823
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| 020 |
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|a 9781483269504
|q (electronic bk.)
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|a 1483269507
|q (electronic bk.)
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|z 0124702074
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|z 9780124702073
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| 035 |
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|a (OCoLC)892069067
|z (OCoLC)898422339
|z (OCoLC)948610823
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4 |
|a QR360
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| 072 |
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|a SCI
|x 027000
|2 bisacsh
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| 082 |
0 |
4 |
|a 576.64
|2 22
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| 245 |
0 |
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|a Methods in virology /
|c edited by Karl Maramorosch and Hilary Koprowski. Vol. 7.
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| 264 |
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1 |
|a New York ;
|a London :
|b Academic Press,
|c 1984.
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| 300 |
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|a 1 online resource (xvi, 332 pages) :
|b illustrations
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 588 |
0 |
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|a Print version record.
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0 |
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|a Front Cover; Methods in Virology; Copyright Page; Table of Contents; Contributors; Preface; Contents of Previous Volumes; Chapter 1. Monoclonal Antibody Techniques Applied to Viruses; I. Introduction; II. Immunization Protocol; III. Preparation of Cells; IV. Hybridization; V. Maintenance and Specificity Testing of Hybridomas; VI. Cloning of Hybridomas; VII. Determination of Immunoglobulin Class; VIII. Preparation of Ascites Fluid; IX. Concentration and Purification of Immunoglobulins; X. Characterization of Viral Antigens Using Monoclonal Antibodies; XI. Prospectus.
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| 505 |
8 |
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|a XII. Appendix: Preparation of ReagentsREFERENCES; Chapter 2. Competition Radioimmunoassays forCharacterization of Antibody Reactionsto Viral Antigens; I. Introduction; II. Preparation and Characterization of Reagents; III. Basic Methodologies; IV. Applicability to Identification of Antibody Populations; V. Applicability to Characterization of Viral Antigens; VI. Biological Tracking of Viral Proteins; VII. Concluding Comments; ACKNOWLEDGMENT; REFERENCES; Chapter 3. Enzyme Immunosorbent Assays in Plant Virology; I. Introduction; II. Principles of Enzyme Immunosorbent Assay.
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| 505 |
8 |
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|a III. Preparation of SampleIV. Production of Antiserum; V. Choice of Enzyme and Preparation of Conjugate; VI. Substrates; VII. Choice of Solid Phase; VIII. Types of ELISA Procedure; IX. Evaluation of Reagents and Analysis of Results; X. Conclusions; XI. Appendix: Buffer Formulations; REFERENCES; Chapter 4. Electron Microscopy for the Identification of Plant Viruses inin Vitro Preparations; I. Introduction; II. The Electron Microscope; III. Support Films; IV. Calibration of Magnification; V. Negative Staining; VI. Metal Shadowing; VII. Immunoelectron Microscopy; VIII. Imaging of Nucleic Acids.
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|a ACKNOWLEDGMENTSREFERENCES; Chapter 5. Cloning and Expression of ViralAntigens in Escherichia coli and Other Microorganisms; I. Introduction; II. Expression of Foreign Coding Sequences in Escherichia coli; III. Hepatitis B Virus; IV. Foot-and-Mouth Disease Virus; V. Poliovirus: Purification of Polio RNA and Cloning of Polio cDNA; VI. Influenza Virus; VII. Rabies Virus; VIII. Expression of Viral Antigens in Bacillus subtilis; IX. Expression of Viral Antigens in Saccharomyces cerevisiae; X. Conclusion; ACKNOWLEDGMENTS; REFERENCES; Chapter 6. Spot Hybridization for Detection of Viroids and Viruses.
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|a I. IntroductionII. PSTV Diagnosis by Nucleic Acid Spot Hybridization; III. Application to Other Viroid Diseases; IV. Application to Viral Diseases; V. Supplementary Methodologies; REFERENCES; Chapter 7. Detection of Viral Nucleic Acids by in Situ Hybridization; I. Introduction; II. Materials and Methods; III. Applications; ACKNOWLEDGMENTS; REFERENCES; Chapter 8. Exploring the Gene Organization of Baculoviruses; I. Introduction; II. Establishing the Physical Map of a Baculovirus; III. Mapping Mutuations of Baculoviruses; IV. Correlating Proteins with the Physical Map.
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| 650 |
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0 |
|a Virology.
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| 650 |
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0 |
|a Viruses.
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| 650 |
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2 |
|a Virology
|0 (DNLM)D014773
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| 650 |
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2 |
|a Viruses
|0 (DNLM)D014780
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| 650 |
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6 |
|a Virologie.
|0 (CaQQLa)201-0008933
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| 650 |
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6 |
|a Virus.
|0 (CaQQLa)201-0078047
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| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Evolution.
|2 bisacsh
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| 650 |
|
7 |
|a Virology
|2 fast
|0 (OCoLC)fst01167670
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| 650 |
|
7 |
|a Viruses
|2 fast
|0 (OCoLC)fst01167771
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| 700 |
1 |
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|a Maramorosch, Karl.
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| 700 |
1 |
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|a Koprowski, Hilary.
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| 776 |
0 |
8 |
|i Print version:
|t Methods in virology
|z 0124702074
|w (OCoLC)605983416
|
| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/book/9780124702073
|z Texto completo
|
