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Transgenic animal technology : a laboratory handbook /
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal cons...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Burlington :
Elsevier Science,
2014.
|
| Edición: | 3rd ed. |
| Colección: | Elsevier insights.
|
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 072 | 7 | |a TEC |x 009010 |2 bisacsh | |
| 082 | 0 | 4 | |a 660.65 |2 23 |
| 245 | 0 | 0 | |a Transgenic animal technology : |b a laboratory handbook / |c edited by Carl A. Pinkert. |
| 250 | |a 3rd ed. | ||
| 260 | |a Burlington : |b Elsevier Science, |c 2014. | ||
| 300 | |a 1 online resource (715 pages) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Elsevier insights | |
| 588 | 0 | |a Print version record. | |
| 520 | |a Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engine. | ||
| 505 | 0 | |a Front Cover; Transgenic Animal Technology; Copyright Page; Contents; List of Contributors; Preface; One: Overview; 1 Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; Two: Transgenic Animal Production Focusing on the Mouse Model; 2 DNA Microinjection, Embryo Handling, and Germplasm Preservation; I. Introduction; II. General Methods; A. DNA Preparation and Purification; 1. DNA Construct/Fragment Structure; 2. Factors that Influence Transgenic Mouse Production; 3. Preparation of DNA for Microinjection. | |
| 505 | 8 | |a A. Construction and Isolation of DNAb. DNA Purification; c. Purification and Quantification of DNA; B. Superovulation, Egg Culture, and Harvest; 1. Mouse Strains for Microinjection and Transgenic Models; a. Strains; b. Genetic Variability and Transgene Expression; 2. Superovulation of Mice; a. Pregnant Mare Serum Gonadotropin and Human Chorionic Gonadotropin; b. Follicle Stimulating Hormone and Luteinizing Hormone Delivered with Osmotic Pumps; 3. Production of Eggs from Superovulated Females; a. Colony; b. Influence of Litter Number; 4. In Vitro Culture of Superovulated Eggs; a. Equipment. | |
| 505 | 8 | |a B. Atmosphere for Culturing Eggsc. Glass Pipettes for Manipulating Eggs; d. Media; e. Water Sources; f. Culture Dishes; 5. Harvesting Superovulated Eggs; a. Mating; b. Oviduct Dissection; c. Release of Eggs from the Oviduct; d. Egg Development and Microinjection Timing; e. Efficiency of Egg Culture; C. Microinjection Needles and Slides; 1. Types of Glass; 2. Preparation of Glass; 3. Pipette Pullers; 4. Injection Pipette Shapes; 5. Beveling Hollow Capillary Microinjection Needles; 6. Microforge Shaping of Pipettes; a. Manufacturers; b. Heating Filament; c. Holding Pipette; d. Injection Pipette. | |
| 505 | 8 | |a 7. Microinjection Slide for Eggsa. Slide Designs; b. Microinjection Media; c. Arrangement of DNA and Media Drops; D. Microinjection Equipment; 1. Microscopes; 2. Micromanipulators; a. Micropipette Holders; b. Micromanipulator Adjustments; c. Antivibration Tables; 3. Microinjection Systems; a. General; b. Oil-Driven Injection Assembly; 4. Oil-Driven Holding Pipettes; 5. Electronic Delivery Systems; E. Microinjection Procedure; 1. Preparation of Injection and Holding Pipettes (Micrometer Syringe System); a. Holding Pipette; b. Injection Pipette; c. Aligning the Injection Needle. | |
| 505 | 8 | |a D. Loading DNA into the Injection Needle2. Preparing Eggs for Microinjection; 3. Steps for Efficient Manipulation of Eggs During Microinjection; a. General; b. Micromanipulators; c. Microinjection Needle; d. Controls and Egg Manipulation; 4. Visualizing and Injecting the Egg Pronuclei; a. Orientation; b. Egg Membrane Penetration with the Injection Needle; c. Filling the Pronuclei with DNA Solution; 5. Criteria for Microinjection to Yield Transgenic Mice; a. Treatment After Microinjection; b. Egg Lysis; c. Record Keeping; d. In Vitro Culture of Microinjected Eggs; F. Egg Transfer. | |
| 650 | 0 | |a Animal genetic engineering. | |
| 650 | 0 | |a Transgenic animals. | |
| 650 | 0 | |a Animal biotechnology. | |
| 650 | 2 | |a Animals, Genetically Modified |0 (DNLM)D030801 | |
| 650 | 6 | |a G�enie g�en�etique animal. |0 (CaQQLa)201-0153411 | |
| 650 | 6 | |a Animaux transg�eniques. |0 (CaQQLa)201-0153412 | |
| 650 | 6 | |a Biotechnologie animale. |0 (CaQQLa)201-0183886 | |
| 650 | 7 | |a SCIENCE |x Chemistry |x Industrial & Technical. |2 bisacsh | |
| 650 | 7 | |a TECHNOLOGY & ENGINEERING |x Chemical & Biochemical. |2 bisacsh | |
| 650 | 7 | |a Animal biotechnology |2 fast |0 (OCoLC)fst00809103 | |
| 650 | 7 | |a Animal genetic engineering |2 fast |0 (OCoLC)fst00809192 | |
| 650 | 7 | |a Transgenic animals |2 fast |0 (OCoLC)fst01154666 | |
| 655 | 2 | |a Laboratory Manual |0 (DNLM)D020484 | |
| 655 | 7 | |a Laboratory manuals |2 fast |0 (OCoLC)fst01920776 | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf |0 (CaQQLa)RVMGF-000001214 | |
| 700 | 1 | |a Pinkert, Carl A., |e editor. | |
| 776 | 0 | 8 | |i Erscheint auch als: |n Druck-Ausgabe |a Pinkert, Carl A. Transgenic Animal Technology . |t A Laboratory Handbook |
| 830 | 0 | |a Elsevier insights. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780124104907 |z Texto completo |