Neuropeptide technology : synthesis, assay, purification, and processing /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Conn, P. Michael
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1991.
Colección:Methods in neurosciences ; v. 6.
Temas:
Neuropeptides > Technique.
Gene expression > Technique.
Neuropeptides > Methodology.
Neuropeptides > biosynthesis
Neuropeptides > chemical synthesis
Neuropeptides > isolation & purification
Neuropeptides > M�ethodologie.
Expression g�enique > Technique.
Gene expression > Technique
Neuropeptide
Acceso en línea:Texto completo
Texto completo
Tabla de Contenidos:
  • Cover image; Title page; Table of Contents; Copyright page; Contributors to Volume 6; Preface; Methods in Neurosciences; Chapter [1]: Large-Scale Synthesis of Gonadotropin-Releasing Hormone Antagonists for Clinical Investigations; Publisher Summary; Introduction; Nal-Glu Antagonist; Azaline; Chemicophysical Characterization of Nal-Glu Antagonist and Azaline; Acknowledgments; Chapter [2]: Synthesis of Peptide Substrates for Protein Kinase C; Publisher Summary; Introduction; Preparation of Peptide Substrate; Assay Method; Application to Crude Extract; Acknowledgments
  • Chapter [3]: Synthesis of Glycosyl NeuropeptidesPublisher Summary; Introduction; Glycosylation Methodologies; General Comments; Reagents and Solvents; Acknowledgments; Chapter [4]: Assays for Biosynthesis of Thyrotropin-Releasing Hormone; Publisher Summary; Introduction; Transfection of AtT20 Cell Line with PreproTRH cDNA; Pulse-Chase Studies of ProTRH-Derived Peptides; Appendix; Acknowledgment; Chapter [5]: Microsequence Analysis of Proteins Purified by Gel Electrophoresis; Publisher Summary; Introduction; Outline of Procedure; Discussion; Experimental Procedures; Acknowledgments
  • Chapter [6]: Preparation of Site-Specific Peptide Immunogens Using Multiple Antigen Peptide Approach SystemPublisher Summary; Introduction; Multiple Antigen Peptide Design; Direct Approach; Indirect Approach; Experimental Procedures; Copy Number of Dendritic Arms; Relationship of Orientation and Antigenicity; Conclusion; Acknowledgments; Chapter [7]: Subcellular Distribution of Neuropeptides and Measurement of Their in Vitro Release; Publisher Summary; General Introduction; Subcellular Fractionation; Neuropeptide Release
  • Chapter [8]: Analysis of Tachykinin Peptide Family Gene Expression Patterns by Combined High-Performance Liquid Chromatography-RadioimmunoassayPublisher Summary; Diversity of Mammalian Tachykinin Peptides; Preparation of Synthetic Tachykinin Peptides and Precursor Fragments; High-Performance Liquid Chromatography Systems for Fractionation of Mammalian Tachykinin Peptides; Extraction of Tachykinin Peptides from Tissues and Cells; Radioimmunoassay of Tachykinin Peptides; Combined HPLC-RIA of Tachykinin Peptides from Tissue Extracts; Acknowledgments
  • Chapter [9]: Ultrastructural Localization of Peptides: Comparison of MethodsPublisher Summary; Introduction; Methods; Results; Discussion; Acknowledgments; Chapter [10]: Measurement of Immune System in Response to Peptides in Central Nervous System; Publisher Summary; Introduction; Methods; Results and Discussion; Acknowledgments; Chapter [11]: Identification of Functionally Significant Phosphorylation Sites on Neuronal Proteins and Preparation of Antibodies That Recognize Them; Publisher Summary; Introduction; Identification of Functionally Significant Phosphorylation Sites

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