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A practical guide to protein and peptide purification for microsequencing /
Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" I...
| Clasificación: | Libro Electrónico |
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| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1993.
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| Edición: | 2nd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
| Sumario: | Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Key Features Highlights of the Second Edition:. Mass spectrophotometric characterization of proteins and peptides. N-terminal sequencing of proteins with blocked N termini. Internal amino acid sequence analysis after protease digestion in-gel and on-blot. Improved microscale peptide purification methods. Computer analysis of protein sequences. New protocols tested and refined through everyday use in authors' laboratories. Updated reference chapter covering all aspects of protein microsequencing |
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| Descripción Física: | 1 online resource (xx, 184 pages) : illustrations |
| Bibliografía: | Includes bibliographical references (pages 159-184). |
| ISBN: | 9780080924618 0080924611 |