Nanomedicine. infectious diseases, immunotherapy, diagnostics, antifibrotics, toxicology and gene medicine / Volume 509 :

This volume in the Methods in Enzymology series comprehensively covers Infectious Diseases, Immunotheraphy, Gene Medicine, Diagnostics and Toxicology of Nanomedicine. With an international board of authors, this volume is split into sections that cover subjects such as Nanomedicines in Immunotherapy...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: D�uzg�une�s, Nejat
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Oxford : Academic/Elsevier, 2012.
Edición:1st edition.
Colección:Methods in enzymology ; volume 509.
Temas:
Nanomedicine.
Nanotechnology.
Nanomedicine
Nanotechnology
Nanom�edecine.
Nanotechnologie.
Internet Resources.
Acceso en línea:Texto completo
Texto completo
Tabla de Contenidos:
  • 1.Enhanced Antiviral Activity of Acyclovir Loaded into Nanoparticles / David Lembo
  • 1.Introduction
  • 2.Preparation of Acyclovir-Loaded Nanoparticles and Fluorescent Nanoparticles
  • 3.Biocompatibility Assays
  • 4.Assessment of the Antiviral Activity
  • 5.Conclusions
  • Acknowledgements
  • References
  • 2.Gold manno-Glyconanoparticles for Intervening in HIV gp120 Carbohydrate-Mediated Processes / Soledad Penades
  • 1.Introduction
  • 2.Design, Preparation, and Characterization of manno-GNPs
  • 3.Evaluation of manno-GNPs as Inhibitors of Carbohydrate-Mediated gp120 Interactions
  • 4.Validation of manno-GNPs as Inhibitors of HIV-1/Cell Infection
  • Acknowledgments
  • References
  • 3.Nanoparticle-Mediated Targeted Delivery of Antiretrovirals to the Brain / Stanley A. Schwartz
  • 1.Introduction
  • 2.Preparation and Characterization of QD
  • 3.Physical Characterization of QDs
  • 4.Preparation of QD-Amprenavir-Tf Nanoplex
  • 5.Assessment of Amprenavir Levels Using HPLC
  • 6.Evaluating the Efficacy of Antiretroviral Containing Nanoplex Using an In Vitro BBB Model
  • 7.Evaluation of the Antiviral Efficacy of the QD-Tf-Amprenavir Nanobioconjugate
  • 8.Concluding Remarks
  • Acknowledgments
  • References
  • 4.Antibacterial Activity of Doxycycline-Loaded Nanoparticles / Sanjeeb K. Sahoo
  • 1.Introduction
  • 2.Polymeric Nanoparticles
  • 3.Chitosan Nanoparticles
  • 4.Polyalkylcyanoacrylate Nanoparticles
  • 5.poly(d,l-lactide-co-glycolide) Nanoparticles
  • 6.poly([MARC+66]-caprolactone) Nanoparticles
  • 7.Doxycycline-Loaded PLGA:PCL Nanoparticles
  • 8.Conclusion
  • References
  • 5.Antimicrobial Properties of Electrically Formed Elastomeric Polyurethane-Copper Oxide Nanocomposites for Medical and Dental Applications / R. P. Allaker
  • 1.Introduction
  • 2.Materials and Methods
  • 3.Results and Discussion
  • 4.Concluding Remarks and Future Work
  • Acknowledgments
  • References
  • 6.Gastrointestinal Delivery of Anti-inflammatory Nanoparticles / Didier Merlin
  • 1.Introduction
  • 2.Material According to Drug Application
  • 3.Anti-inflammatory Compounds as Encapsulated Drug
  • 4.NSAIDs-Loaded NPs
  • 5.Biomaterial Choice
  • 6.Targeting NPs to the Colon: Hydrogel-Encapsulated NPs
  • 7.Conclusion
  • Acknowledgments
  • References
  • 7.Chitosan-Based Nanoparticles as a Hepatitis B Antigen Delivery System / Olga Borges
  • 1.Introduction
  • 2.Chitosan-Based Particle Preparation
  • 3.Physicochemical Characterization of the Particles
  • 4.Antigen Adsorption Studies
  • 5.In vitro Release Studies
  • 6.Evaluation of the Bioactivity of the Antigen
  • 7.Cell Viability Studies with Spleen Cells
  • 8.Studies on Uptake into Peyer's Patches
  • 9.Concluding Remarks
  • References
  • 8.Targeting Nanoparticles to Dendritic Cells for Immunotherapy / Carl G. Figdor
  • 1.Introduction
  • 2.Passive Targeting
  • 3.Active Targeting
  • 4.Particulate Vaccines
  • 5.Targeting Gold NP Vaccines to DCs
  • 6.Experimental Procedure for Targeted AuNP Preparation
  • 7.Targeting Liposome-Based Vaccines to DCs
  • 8.Targeting Poly(Lactic-co-Glycolic Acid)-Based Vaccines to DCs
  • 9.Experimental Procedure for the Preparation of Targeted PLGA NP
  • 10.Conclusion
  • References
  • 9.Protein-Carbon Nanotube Sensors: Single Platform Integrated Micro Clinical Lab for Monitoring Blood Analytes / V. Renugopalakrishnan
  • 1.Introduction
  • 2.Prototype
  • 3.Concept
  • 4.Biosensors
  • 5.Microfluidics
  • 6.Amperometric Sensor for the Detection of Blood Analytes
  • 7.Protein Probes for Detection and Monitoring Molecular Components of Serum
  • 8.Protein Engineering and Molecular Biology of Probe Proteins
  • 9.Control of the Fermentation Process
  • 10.Gene Structure and Purification of Overexpressed Protein
  • 11.Immobilization of Proteins on SWCNT
  • 12.Immobilization of Proteins on Self-Assembled Monolayers
  • 13.Characterization of SWCNT Enzyme Adduct
  • 14.Mediators
  • 15.Surface Characterization
  • 16.Fabrication
  • 17.Signal Detection
  • 18.Experimental Protocol
  • 19.Conclusion
  • Acknowledgments
  • References
  • 10.Investigating the Toxic Effects of Iron Oxide Nanoparticles / Kevin Braeckmans
  • 1.Introduction
  • 2.Materials
  • 3.Methods
  • 4.Concluding Remarks
  • Acknowledgments
  • References
  • 11.Cytotoxicity of Gold Nanoparticles / Willi Jahnen-Dechent
  • 1.Introduction
  • 2.Dosage and Quantification of Gold Nanoparticles
  • 3.Aggregation State of Nanoparticles in Fluids
  • 4.Cell-Based Nanotoxicity Studies
  • References
  • 12.Design of Target-Seeking Antifibrotic Compounds / Tero A. H. Jarvinen
  • 1.Introduction
  • 2.In Vivo Phage Display
  • 3.Homing Peptides
  • 4.Multifunctional Fusion Proteins
  • 5.Function of Multifunctional Fusion Protein
  • 6.Concluding Remarks
  • Acknowledgments
  • References
  • 13.Design and Fabrication of N-Alkyl-Polyethylenimine-Stabilized Iron Oxide Nanoclusters for Gene Delivery / Xiaoyuan Chen
  • 1.Introduction
  • 2.Materials
  • 3.Methods
  • 4.Notes
  • 5.Anticipated Results
  • 6.Summary
  • Acknowledgments
  • References
  • 14.Cell-Penetrating Peptide-Based Systems for Nucleic Acid Delivery: A Biological and Biophysical Approach / Maria C. Pedroso de Lima
  • 1.Introduction
  • 2.Methods for Preparation of CPP-Based Nucleic Acid Complexes
  • 3.Methods for Physical Characterization of CPP-Based Nucleic Acid Complexes and Their Interactions with Membranes
  • 4.Methods for Evaluation of Biological Activity of CPP-Based Nucleic Acid Complexes
  • 5.Methods for Evaluation of Cytotoxicity of CPP-Based Nucleic Acid Complexes
  • 6.Concluding Remarks
  • Acknowledgments
  • References
  • 15.Multifunctional Envelope-Type Nano Device (MEND) for Organelle Targeting Via a Stepwise Membrane Fusion Process / Hideyoshi Harashima
  • 1.Programmed Packaging Concept and Construction of R8-MEND
  • 2.Screening of Lipid Compositions for Their Ability to Fuse with Nuclear and Mitochondrial Membranes
  • 3.Construction of Tetra-Lamellar MEND (T-MEND)
  • 4.Nuclear Gene Delivery
  • 5.Mitochondrial Bioactive Molecule Delivery Using a Dual Function-MITO-Porter as a MEND for Mitochondrial Delivery
  • 6.Conclusions
  • Acknowledgments
  • References
  • 16.Lipopolyplexes as Nanomedicines for Therapeutic Gene Delivery / Conchita Tros de Ilarduya
  • 1.Introduction
  • 2.Principle of the Method
  • 3.Experimental Procedures
  • 4.Application of Lipopolyplexes
  • 5.Concluding Remarks
  • Acknowledgments
  • References
  • 17.Interfering Nanoparticles for Silencing MicroRNAs / Tariq M. Rana
  • 1.Introduction
  • 2.Delivery of Short Therapeutic RNAs
  • 3.Protocols
  • 4.Concluding Remarks
  • Acknowledgments
  • References
  • 18.Genetic Nanomedicine: Gene Delivery by Targeted Lipoplexes / Conchita Tros de Ilarduya
  • 1.Introduction
  • 2.Preparation of Protein-Cationic Lipid-DNA Ternary Complexes
  • 3.Gene Delivery In Vitro
  • 4.Cell Viability Following Transfection
  • 5.Enhancement of Transfection In Vitro
  • 6.Targeted Lipoplexes In Vivo
  • 7.Concluding Remarks
  • Acknowledgments
  • References.

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