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Neuroanatomical research techniques /
Neuroanatomical Research Techniques discusses developments in major neuroanatomical research techniques. The book is organized into four parts. Part I deals generally with the preparation and study of brain tissue. It includes a chapter on the microscope, discussing optical magnification, limitation...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York :
Academic Press,
1978.
|
| Colección: | Methods in physiological psychology ;
v. 2. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Neuroanatomical Research Techniques; Copyright Page; Table of Contents; List of Contributors; Foreword; Preface; Part 1: Preparation and Study of Brain Tissues; Chapter 1. Microscopy and Photomicrography: An Introduction; I. The Use of the Microscope; II. Photographic Techniques; Chapter 2. Tissue Preparation and Basic Staining Techniques; I. Introduction; II. Tissue Fixation; III. Preparation of Tissues for Sectioning; IV. Mounting of Sections; V. Staining Procedures; VI. Conclusions; References; Chapter 3. Computer-Assisted Recording of Neuroanatomical Data; I. Introduction
- II. Suitability of Computers to Recording Neuroanatomical DataIII. Methodology; IV. Appearance of the Data, Analysis, and Interpretation; V. Technical Problems, Methodological Limitations, and Interpretive Difficulties; VI. Summary and Comments; Acknowledgments; References; Part 2: Techniques for the Study of Normal Tissue; Chapter 4. The Methods of Golgi; I. Introduction; II. Why Use the Golgi Methods?; III. Where Is the Golgi Useful?; IV. How to Use the Golgi Methods; V. What Are the Artifacts and Pitfalls?; VI. Rationale of the Golgi Methods; VII. Interpretation of the Data
- AcknowledgmentsReferences; Chapter 5. Fluorescence Histochemistry; I. Introduction; II. Fluorescence Histochemical Methods for Demonstrating Biogenic Amines in Neurons; III. Conclusions; Acknowledgments; References; Chapter 6. Single-Cell Staining Techniques; I. Introduction; II. Microelectrode Injection; III. Axonal Iontophoresis: Procedure; IV. Tissue Processing for Light Microscopy; V. Ultrastructural Use of Intracellular Dyes; VI. Possible Limitations of Intracellular Staining; VII. Prospectus; VIII. Addendum; Acknowledgments; References
- Chapter 7. Electron Microscopy and the Study of the Ultrastructure of the Central Nervous SystemI. Introduction; II. Specimen Preparation; III. Additional Selective Methods for Electron Microscopy; IV. Ultrastructure: The Interpretation of the Electron Microscopic Image; Acknowledgments; References; Part 3: Study of Connections in the Nervous System; Chapter 8. The Study of Degenerating Nerve Fibers Using Silver-Impregnation Methods; I. The Need for the Silver-Impregnation Methods; II. Methodology; III. Appearance and Analysis of the Material; IV. Limitations and Artifacts of the Methods
- v. Interpretation of the ResultsAcknowledgments; References; Chapter 9. The Use of Axonal Transport for Autoradiographic Tracing of Pathways in the Central Nervous System; I. The Technique and the Problems For Which It Is Appropriate; II. Methodology of Autoradiographic Nerve Tracing (ARNT); III. Other Uses for Isotopes in Nerve Tracing; IV. Appearance and Analysis of Data; V. Limitations and Artifacts of ARNT; Acknowledgments; References; Chapter 10. The Use of Somatofugal Transport of Horseradish Peroxidase for Tract Tracing and Cell Labeling; I. Introduction