Gel electrophoresis of proteins /

Gel Electrophoresis of Proteins.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Dunn, M. J.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Bristol : Wright, 1986.
Temas:
Proteins > Purification.
Gel electrophoresis.
Electrophoresis.
Proteins > Analysis.
Electrophoresis, Polyacrylamide Gel > methods
Proteins > analysis
Electrophoresis
�Electrophor�ese.
Prot�eines > Analyse.
SCIENCE > Life Sciences > Biology.
Proteins > Analysis
Gel electrophoresis
Proteins > Purification
Gelelektrophorese
Proteine
Prot�eines > �Electrophor�ese.
�Electrophor�ese sur gel.
Proteins > Chemical analysis > Gel electrophoresis
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover; Gel Electrophoresis of Proteins; Copyright Page; Preface; List of Contributors; Table of Contents; List of Abbreviations; Chapter 1. Steady-state Gel Electrophoresis Systems; 1.1 Introduction; 1.2 Historical Developments; 1.3 Fundamental Steady-state Electrophoresis Systems; 1.4 Fundamental Properties of Steady-state Electrophoresis Systems; 1.5 Nomenclature and Definitions; 1.6 Conductivity; 1.7 Equations for Calculation of System Composition; 1.8 Isotachophoresis; 1.9 Moving Boundary Electrophoresis; 1.10 Isoelectric Focusing Systems; Acknowledgements; References
  • Chapter 2. One-dimensional PAA-gel Electrophoretic Techniques to Separate Functional and Denatured Proteins2.1 Introduction; 2.2 Structure and Physico-chemical Properties of Polyacrylamide Gels; 2.3 Analytical Polyacrylamide Gel Electrophoresis; 2.4 Affinity Electrophoresis; Acknowledgments; References; Chapter 3. Conventional Isoelectric Focusing and Immobilised pH Gradients; 3.1 Introduction; 3.2 The Principles of IEF; 3.3 IEF in Agarose Matrices; 3.4 IEF in Polyacrylamide Matrices; 3.5 Titration Curves; 3.6 Production of Narrow pH Gradients; 3.7 Measurements of pH Gradients
  • 3.8 Trouble Shooting3.9 Artefacts: a Unified View; 3.10 The Chemicals; the Immobiline Matrix; 3.11 Narrow and Ultra-narrow pH Gradients; 3.12 Extended pH Gradients; 3.13 On Buffering Capacity and Ionic Strength; 3.14 On Electro-endosmosis; 3.15 Polymerisation Kinetics; 3.16 Methodology: Casting an Immobiline Gel; 3.17 Artefacts; 3.18 Conclusions; Acknowledgments; References; Chapter 4. High Resolution Two-dimensional Polyacrylamide-gel Electrophoresis; 4.1 Introduction; 4.2 Two-dimensional Electrophoresis under Non-denaturing Conditions
  • 4.3 Denaturing 2D Systems using Electrophoresis in both Dimensions4.4 The O'Farrell System; 4.5 Sample Preparation and Solubilisation; 4.6 IEF Dimension; 4.7 New Alternatives for the First Dimension of 2D PAGE; 4.8 Equilibration; 4.9 SDS Dimension; 4.10 Visualisation, Detection and Analysis; 4.11 Heterogeneity and Artefacts; 4.12 Estimates of Resolution; 4.13 Applications; 4.14 2D-PAGE and the Study of Human Genetic Disorders; Acknowledgments; References; Chapter 5. Immunoelectrophoretic Methods; 5.1 Introduction; 5.2 Historical Background; 5.3 Theory; 5.4 General Conditions and Guidelines
  • 5.5 Contemporary Methods5.6 Applicability of Immunoelectrophoretic Methods; 5.7 Advantages and Drawbacks; References; Chapter 6. In situ Peptide Mapping of Proteins and Polypeptides Separated by Polyacrylamide Gel Electrophoresis; 6.1 Introduction; 6.2 Primary Gel Systems; 6.3 Storage, Rehydration and Equilibration; 6.4 Protein Cleavage; 6.5 Secondary Gel Systems; 6.6 Detection; 6.7 Evaluation; References; Chapter 7. Protein Staining and Detection Methods; 7.1 Historical Introduction; 7.2 Post-Electrophoretic Organic Protein Stains; 7.3 Pre-electrophoretic Organic Stains; 7.4 Silver Stains

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