Methods in plant molecular biology /

Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protei...

Full description

Bibliographic Details
Call Number:Libro Electrónico
Main Author: Schuler, Mary A.
Other Authors: Zielinski, Raymond E.
Format: Electronic eBook
Language:Inglés
Published: San Diego : Academic Press, �1989.
Subjects:
Plant molecular biology > Experiments.
Biologie mol�eculaire v�eg�etale > Exp�eriences.
Pflanzen
Molekularbiologie
Praktikum
Methode
Online Access:Texto completo
Table of Contents:
  • Front Cover; Methods in Plant Molecular Biology; Copyright Page; Table of Contents; Preface; Laboratory Schedule; Chapter 1. Restriction Mapping of Plasmid DNA; Introduction; Protocols; Materials Provided; References; Chapter 2. Cloning of Restriction Fragments into pUC Vectors; Introduction; Protocols; Materials Provided; References; Chapter 3A. Preparation of Intact Chloroplasts from Pea; Introduction; Protocols; Materials Provided; References; Chapter 3B. Lowry Assay for Protein Determination; Introduction; Protocol; Materials Provided; Reference
  • Chapter 3C. Protein Synthesis by Isolated Pea ChloroplastsIntroduction; Special Precautions for Use of Radioisotopes; Protocol; Materials Provided; References; Chapter 3D. Separation of Thylakoid and Stromal Proteins by SDS-Gel Electrophoresis; Introduction; Protocols; Materials Provided; References; Chapter 4. Isolation of Chloroplast DNA; Introduction; Protocols; Materials Provided; References; Chapter 5A. RNA Isolation from Light- and Dark-Grown Seedlings; Introduction; Protocol; Materials Provided; References
  • Chapter 5B. Preparation of a Wheat Germ Extract for in Vitro Translation of mRNAIntroduction; Special Precautions for Use of Radioisotopes; Protocols; Materials Provided; References; Chapter 6A. Screening of Recombinant Phage Libraries with Cloned CDNA Probes; Introduction; Protocols; Materials Provided; References; Chapter 6B. Isolation of Phage DNA from Liquid Cultures; Introduction; Protocol; Materials Provided; Chapter 7. Dideoxy DNA Sequencing; Introduction; Protocols; Materials Provided; References; Chapter 8. Transformation of Leaf Discs with Agrobacterium; Introduction; Protocols
  • Materials ProvidedReferences; Appendix I: Glossary; Terms and Definitions; Abbreviations; Appendix II: Gel Electrophoresis Equipment; Power Supplies; Electrophoresis Rigs; Index

Similar Items