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Methods in plant molecular biology /
Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protei...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1989.
|
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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|---|---|---|---|
| 001 | SCIDIR_ocn681913986 | ||
| 003 | OCoLC | ||
| 005 | 20231117044530.0 | ||
| 006 | m o d | ||
| 007 | cr bn||||||abp | ||
| 007 | cr bn||||||ada | ||
| 008 | 101117s1989 caua ob 001 0 eng d | ||
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| 020 | |a 9781299196506 |q (ebk) | ||
| 020 | |a 0323137652 | ||
| 020 | |a 9780323137652 | ||
| 020 | |z 0126323402 | ||
| 020 | |z 9780126323405 | ||
| 020 | |z 9780126323405 |q (pbk. ; |q alk. paper) | ||
| 035 | |a (OCoLC)681913986 |z (OCoLC)645889408 |z (OCoLC)842859877 |z (OCoLC)974619851 |z (OCoLC)974670833 |z (OCoLC)1162358071 | ||
| 042 | |a dlr | ||
| 050 | 4 | |a QK728 |b .S38 1989 | |
| 070 | |a QK728.S38 | ||
| 072 | 0 | |a F600 | |
| 082 | 0 | 4 | |a 581.8/0724 |2 19 |
| 100 | 1 | |a Schuler, Mary A. | |
| 245 | 1 | 0 | |a Methods in plant molecular biology / |c Mary A. Schuler, Raymond E. Zielinski. |
| 260 | |a San Diego : |b Academic Press, |c �1989. | ||
| 300 | |a 1 online resource (xv, 171 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references and index. | ||
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 520 | |a Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. Key Features of the Manual * Eight basic experiments which can be used collectively or individually cover * Recombinant Cloning and Screening in E. coli; DNA Sequencing * Plant RNA Isolation and in Vitro Translations * Plant DNA Isolations and Genomic DNA Southern Analysis Chloroplast Isolation and Protein Synthesis Plant Tissue Culture and Agrobacterium Transformations * Experiments that have been student tested for three years * Blueprints for setting up gel rigs * Comprehensive course schedule outlining individual procedures to be finished in each lab segment * Course can be tailored to suit the needs of the individual instructor. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Methods in Plant Molecular Biology; Copyright Page; Table of Contents; Preface; Laboratory Schedule; Chapter 1. Restriction Mapping of Plasmid DNA; Introduction; Protocols; Materials Provided; References; Chapter 2. Cloning of Restriction Fragments into pUC Vectors; Introduction; Protocols; Materials Provided; References; Chapter 3A. Preparation of Intact Chloroplasts from Pea; Introduction; Protocols; Materials Provided; References; Chapter 3B. Lowry Assay for Protein Determination; Introduction; Protocol; Materials Provided; Reference | |
| 505 | 8 | |a Chapter 3C. Protein Synthesis by Isolated Pea ChloroplastsIntroduction; Special Precautions for Use of Radioisotopes; Protocol; Materials Provided; References; Chapter 3D. Separation of Thylakoid and Stromal Proteins by SDS-Gel Electrophoresis; Introduction; Protocols; Materials Provided; References; Chapter 4. Isolation of Chloroplast DNA; Introduction; Protocols; Materials Provided; References; Chapter 5A. RNA Isolation from Light- and Dark-Grown Seedlings; Introduction; Protocol; Materials Provided; References | |
| 505 | 8 | |a Chapter 5B. Preparation of a Wheat Germ Extract for in Vitro Translation of mRNAIntroduction; Special Precautions for Use of Radioisotopes; Protocols; Materials Provided; References; Chapter 6A. Screening of Recombinant Phage Libraries with Cloned CDNA Probes; Introduction; Protocols; Materials Provided; References; Chapter 6B. Isolation of Phage DNA from Liquid Cultures; Introduction; Protocol; Materials Provided; Chapter 7. Dideoxy DNA Sequencing; Introduction; Protocols; Materials Provided; References; Chapter 8. Transformation of Leaf Discs with Agrobacterium; Introduction; Protocols | |
| 505 | 8 | |a Materials ProvidedReferences; Appendix I: Glossary; Terms and Definitions; Abbreviations; Appendix II: Gel Electrophoresis Equipment; Power Supplies; Electrophoresis Rigs; Index | |
| 546 | |a English. | ||
| 650 | 0 | |a Plant molecular biology |x Experiments. | |
| 650 | 6 | |a Biologie mol�eculaire v�eg�etale |0 (CaQQLa)201-0158080 |x Exp�eriences. |0 (CaQQLa)201-0377433 | |
| 650 | 7 | |a Plant molecular biology |x Experiments. |2 fast |0 (OCoLC)fst01065558 | |
| 650 | 7 | |a Pflanzen |2 gnd |0 (DE-588)4045539-7 | |
| 650 | 7 | |a Molekularbiologie |2 gnd |0 (DE-588)4039983-7 | |
| 650 | 7 | |a Praktikum |2 gnd |0 (DE-588)4127380-1 | |
| 650 | 7 | |a Methode |2 gnd |0 (DE-588)4038971-6 | |
| 700 | 1 | |a Zielinski, Raymond E. | |
| 776 | 0 | 8 | |i Print version: |a Schuler, Mary A. |t Methods in plant molecular biology. |d San Diego : Academic Press, �1989 |w (DLC) 88012128 |w (OCoLC)17841191 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780126323405 |z Texto completo |