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Genetics and biotechnology of bacilli, volume 2 /
GENETICS & BIOTECHNOLOGY OF BACILLI V2 Z.
| Call Number: | Libro Electrónico |
|---|---|
| Corporate Author: | |
| Other Authors: | , |
| Format: | Electronic Conference Proceeding eBook |
| Language: | Inglés |
| Published: |
San Diego :
Academic Press,
1988.
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| Subjects: | |
| Online Access: | Texto completo |
Table of Contents:
- Front Cover; Genetics and Biotechnology of Bacilli; Copyright Page; Table of Contents; Preface; Part I: Gene Expression; CHAPTER 1. UPSTREAM ACTIVATING SEQUENCES IN BACILLUS SUBTILIS; I. INTRODUCTION; II. THE KNOWN TARGETS; III. THE KNOWN PLAYERS; IV. THE MECHANISM; V. EVIDENCE FOR UPSTREAM ACTIVATION; VI. OTHER PROCARYOTIC SYSTEMS WITH UPSTREAM ACTIVATION; VII. LOOMING QUESTIONS; REFERENCES; CHAPTER 2. LEVANSUCRASE INDUCTION BY SUCROSE IN BACILLUS SUBTILIS INVOLVES AN ANTITERMINATOR. HOMOLOGY WITH THE ESCHERICHIA COLI bgl OPERON; I. INTRODUCTION; II. HOMOLOGY BETWEEN sacY AND bglC
- III. ROLE OF sacS IN SUCRASE REGULATIONIV. INVOLVEMENT OF THE PTS IN sacB INDUCTION; V. CONCLUSION; REFERENCES; CHAPTER 3. INDUCIBLE EXPRESSION SYSTEM BASED ON SUCROSE METABOLISM GENES OF BACILLUS SUBTILIS; I. INTRODUCTION; II. NUCLEOTIDE SEQUENCE OF THE sacS LOCUS; III. CONSTRUCTION OF EXPRESSION VECTORS; IV. INDUCIBLE EXPRESSION OF xylE; V. ACKNOWLEDGEMENT; REFERENCES; CHAPTER 4. DEVELOPMENT OF AN EXPRESSION AND SECRETION SYSTEM IN BACILLUS SUBTILIS UTILIZING sacQ; I. INTRODUCTION; II. CLONING AND MODIFICATION OF THE B. AMYLOLIQUEFACIENS sacQ GENE
- III. MODIFICATION OF THE SUBTILISIN SIGNAL SEQUENCEIV. SUMMARY; REFERENCES; CHAPTER 5. POLYPEPTIDES ACTIVATING THE SYNTHESIS OF SECRETED ENZYMES; I. INTRODUCTION; II. REGULATORY GENES ENCODING SMALL POLYPEPTIDES; III. PROSPECTS; IV. SUMMARY; REFERENCES; CHAPTER 6. ENHANCEMENT OF mRNA LEVEL BY prtR; I. INTRODUCTION; II. ANALYSIS OF prtR PROTEIN; III. TARGET OF prtR; IV. CONCLUSIONS; V. SUMMARY; REFERENCES; CHAPTER 7. REGULATION OF POST EXPONENTIAL GENE EXPRESSION: TRANSCRIPTIONAL CONTROL OF THE SUBTILISIN GENE; I. INTRODUCTION; II. SINGLE COPY INTEGRATION VECTOR IN spoO MUTANTS
- III. TRANSCRIPTION START SITEIV. EFFECT OF THE spoOA AND abrB MUTATIONS ON SUBTILISIN EXPRESSION; V. EFFECT OF THE sacU(h) AND hpr MUTATIONS ON apr mRNA; VI. EFFECT OF SIGMA FACTOR MUTATIONS ON THE SUBTILISIN EXPRESSION; VII. DISCUSSION; REFERENCES; CHAPTER 8. ISOLATION AND CHARACTERIZATION OF A NOVEL BACILLUS SUBTILIS AND BACILLUS NATTO GENE THAT ENHANCES PROTEASE PRODUCTION; I. INTRODUCTION; II. ISOLATION OF THE GENES; III. CHARACTERIZATION OF THE GENES; IV. DISCUSSION; V. SUMMARY; REFERENCES; CHAPTER 9. CHARACTERIZATION OF THE BACILLUS SUBTILIS Pur OPERON: NEW INSIGHTS INTO GENE REGULATION
- I. INTRODUCTIONII. CLONING OF THE BACILLUS SUBTILIS PUR OPERON; III. ORGANIZATION OF THE OPERON; IV. NUCLEASE SI MAPPING EXPERIMENTS; V. CONCLUSIONS; REFERENCES; CHAPTER 10. PURINE GENE ORGANIZATION AND REGULATION IN BACILLUS SUBTILIS; I. INTRODUCTION; II. PURINE SALVAGE PATHWAYS; III. PURINE DE NOVO SYNTHESIS PATHWAY; IV. SUMMARY; V. ACKNOWLEDGMENTS; REFERENCES; CHAPTER 11. MUTATIONS IN THE SPACER REGION OF A BACILLUS SUBTILIS PROMOTER; I. INTRODUCTION; II. OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS; III. DELETION MUTAGENESIS; IV. CONCLUSIONS; V. ACKNOWLEDGMENTS; REFERENCES