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RNA-ligand interactions. Part A, Structural biology methods /
RNA-Ligand Interactions, Part A focuses on structural biology methods. Major topics covered include semisynthetic methodologies (RNA synthetic methods and derivatization of RNA); RNA structure determination (X-ray crystallography, NMR, EM); techniques for monitoring RNA conformation and dynamics (so...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, CA ; London :
Academic Press,
�2000.
|
| Colección: | Methods in enzymology ;
v. 317. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a RNA-ligand interactions. |n Part A, |p Structural biology methods / |c edited by Daniel W. Celander, John N. Abelson. |
| 260 | |a San Diego, CA ; |a London : |b Academic Press, |c �2000. | ||
| 300 | |a 1 online resource (xxxiii, 549 pages [8] pages of color plates) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 490 | 1 | |a Methods in enzymology ; |v v. 317 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 520 | |a RNA-Ligand Interactions, Part A focuses on structural biology methods. Major topics covered include semisynthetic methodologies (RNA synthetic methods and derivatization of RNA); RNA structure determination (X-ray crystallography, NMR, EM); techniques for monitoring RNA conformation and dynamics (solution methods and electrophoretic and spectroscopic methods); and modeling tertiary structure: Part B, its companion Volume 318 of Methods in Enzymology, focuses on molecular biology methods. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the Series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Methods in Enzymology; Copyright Page; Table of Contents; Contributors to Volume 317; Preface; Volumes in Series; Section I: Semisynthetic Methodologies; A. RNA Synthetic Methods; Chapter 1. Advanced 5' -Silyl-2' -Orthoester Approach to RNA Oligonucleotide Synthesis; Chapter 2. Preparation of Specifically 2H- and 13C-Labeled Ribonucleotides; Chapter 3. Base-Modified Phosphoramidite Analogs of Pyrimidine Ribonucleosides for RNA Structure- Activity Studies; B. Derivatization of RNA; Chapter 4. Use of T7 RNA Polymerase and Its Mutants for Incorporation of Nucleoside Analogs into RNA | |
| 505 | 8 | |a Chapter 5. Phosphorothioate Modification of RNA for Stereochemical and Interference AnalysesChapter 6. Chemical Probing of RNA by Nucleotide Analog Interference Mapping; Chapter 7. Joining of RNAs by Splinted Ligation; Chapter 8. Heavy Atom Derivatives of RNA; Chapter 9. Site-Specific Cleavage of Transcript RNA; Chapter 10. Using DNAzymes to Cut, Process, and Map RNA Molecules for Structural Studies or Modification; Section II: RNA Structure Determination; A. X-Ray Crystallography; Chapter 11. Purification, Crystallization, and X-Ray Diffraction Analysis of Small Ribozymes | |
| 505 | 8 | |a Chapter 12. Solving Large RNA Structures by X-Ray CrystallographyChapter 13. Conventional and Time-Resolved Ribozyme X-Ray Crystallography; B. Nuclear Magnetic Resonance Spectroscopy; Chapter 14. Nuclear Magnetic Resonance Methods to Study RNA-Protein Complexes; Chapter 15. Filamentous Bacteriophage for Aligning RNA, DNA, and Proteins for Measurement of Nuclear Magnetic Resonance Dipolar Coupling Interactions; Chapter 16. Biochemical and Nuclear Magnetic Resonance Studies of Aminoglycoside-RNA Complexes; C. Electron Microscopy | |
| 505 | 8 | |a Chapter 17. Experimental Prerequisites for Determination of tRNA Binding to Ribosomes from Escherichia coliChapter 18. Three-Dimensional Cryoelectron Microscopy of Ribosomes; Chapter 19. Preparation of Functional Ribosomal Complexes and Effect of Buffer Conditions on tRNA Positions Observed by Cryoelectron Microscopy; Section III: Techniques for Monitoring RNA Conformation and Dynamics; A. Solution Methods; Chapter 20. Probing RNA Structure and Function by Circular Permutation; Chapter 21. Kinetic Oligonucleotide Hybridization for Monitoring Kinetic Folding of Large RNAs | |
| 505 | 8 | |a Chapter 22. Time-Resolved Synchrotron X-Ray Footprinting and Its Application to RNA FoldingB. Electrophoretic and Spectroscopic Methods; Chapter 23. Analysis of Global Conformation of Branched RNA Species Using Electrophoresis and Fluorescence; Chapter 24. Application of Circular Dichroism to Study RNA Folding Transitions; Chapter 25. Fluorescence Assays to Study Structure, Dynamics, and Function of RNA and RNA-Ligand Complexes; Chapter 26. Transient Electric Birefringence for Determining Global Conformations of Nonhelix Elements and Protein-Induced Bends in RNA | |
| 546 | |a English. | ||
| 650 | 0 | |a Ligands (Biochemistry) | |
| 650 | 0 | |a RNA. | |
| 650 | 0 | |a Ligands. | |
| 650 | 2 | |a Ligands |0 (DNLM)D008024 | |
| 650 | 2 | |a RNA |x physiology |0 (DNLM)D012313Q000502 | |
| 650 | 2 | |a RNA |0 (DNLM)D012313 | |
| 650 | 6 | |a Ligands (Biochimie) |0 (CaQQLa)201-0197601 | |
| 650 | 6 | |a ARN. |0 (CaQQLa)201-0024711 | |
| 650 | 6 | |a Ligands. |0 (CaQQLa)201-0027688 | |
| 650 | 7 | |a Ligands |2 fast |0 (OCoLC)fst00998466 | |
| 650 | 7 | |a Ligands (Biochemistry) |2 fast |0 (OCoLC)fst00998471 | |
| 650 | 7 | |a RNA |2 fast |0 (OCoLC)fst01086244 | |
| 700 | 1 | |a Celander, Daniel W. |q (Daniel Walter) | |
| 700 | 1 | |a Abelson, John. | |
| 830 | 0 | |a Methods in enzymology ; |v v. 317. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780121822187 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/317 |z Texto completo |