Methods in cell biology. Volume 7 /

METHODS IN CELL BIOLOGY,VOLUME 7.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Prescott, David M. (David Martin), 1948-
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Academic Press, 1973.
Colección:Methods in cell biology ; v. 7.
Temas:
Cytology > Technique.
Biology > Technique.
Cytological Techniques
Cytologie > Technique.
Biologie > Technique.
SCIENCE > Life Sciences > Cell Biology.
Biology > Technique
Cytology > Technique
Biologia celular.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Cover; Contributors to This Volume; Contents; List of Contributors; Preface; Contents of Previous Volumes; Chapter 1. The Isolation of RNA from Mammalian Cells; I. Introduction; II. The Isolation of Subcellular Components; III. The Extraction of RNA; IV. RNA Purification Procedures; V. The Isolation of Messenger RNA; References; Chapter 2. Preparation of RNA from Animal Cells; I. Introduction; II. Labeling of RNA with Radioactive Precursors; III. Preparation of Rapidly Labeled Nuclear RNA; IV. Preparation of Ribosomal RNA; V. Preparation of Messenger RNA; VI. Preparation of Transfer RNA
  • VII. Preparation of Mitochondrial RNAVIII. Concluding Remarks; References; Chapter 3. Detection and Utilization of Poly(A) Sequennces in Messenger RNA; I. Introduction; II. Detection and Characterization; References; Chapter 4. Recent Advances in the Preparation of Mammalian Ribosomes and Analysis of Their Protein Composition; I. Introduction; II. Preparation of Ribosomes; III. Preparation of Ribosomal Subunits; IV. Preparation of Total Protein from Ribosomes and Ribosomal Subunits; V. Analysis of Ribosomal Proteins; VI. Molecular Weight Distribution and Number of Ribosomal Proteins
  • VII. Purified ProteinsVIII. Heterogeneity of Ribosomal Proteins; IX. Methods for Correlating Structure and Function; X. Membrane-Bound Ribosomes; XI. Phosphorylation of Ribosomal Proteins; XII. Translation of Specific Species of Messenger RNA into Proteins of Proven Structure; XIII. Concluding Remarks; References; Chapter 5. Methods for the Extraction and Purification of Deoxyribonucleic Acids from Eukaryote Cells; I. Introduction; II. Physical-Chemical Characteristics of DNA; III. General Methodology; IV. Concluding Remarks; V. Glossary of Reagents; References
  • Chapter 6. Electron Microscopic Visualization of DNA in Association with Cellular ComponentsI. Introduction; II. Isolation of DNA-Protein Complexes; III. Electron Microscopy; IV. Direct Visualization of Defined Complexes; V. Phage-Membrane Association: A Combination of Two Methods; VI. Eukaryote Chromatin Fibers; References; Chapter 7. Autoradiography of Individual DNA Molecules; I. Introduction; II. Labeling of DNA; III. Spreading of DNA; IV. Autoradiography of DNA; V. Examples of Autoradiographs; References; Chapter 8. HeLa Cell Plasma Membranes; I. Introduction
  • II. Preparation of HeLa Cell Plasma MembranesIII. Uses of the Method; IV. Discussion; References; Addendum; Chapter 9. Mass Enucleation of Cultured Animal Cells; I. Introduction; II. Cells; III. Manufacture of Plastic Coverslips; IV. Enucleation; V. Viability of Enucleated Cells; VI. Virus Activities in Enucleated Cells; VII. Viability of Nuclei Removed by Centrifugation; References; Chapter 10. The Production of Mass Populations of Anucleate Cytoplasms; I. Introduction; II. Preparation of Lucite Plates; III. Plating of Cells; IV. Enucleation

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