Methods in cell biology. Volume 50, Methods in plant cell biology. Part B /

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Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical descriptio...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Galbraith, David W., Bohnert, Hans J., Bourque, Don P.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1995.
Colección:Methods in cell biology ; v. 50.
Temas:
Cytology > Technique.
Plant cells and tissues.
Cells.
Biology.
Plants
Cells
Biology
Cytological Techniques
Cytologie > Technique.
Plantes > Cellules et tissus.
Plantes.
Cellules.
Biologie.
biology.
vegetation.
SCIENCE > Life Sciences > Cell Biology.
Cytology > Technique
Plant cells and tissues
Plants > Cells
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover; Methods in Cell Biology: Methods in Plant Cell Biology, Part B; Copyright Page; Contents; Contributors; Preface; Part I: Subcellular Fractionation and Analysis of Function; Chapter 1. Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green Fluorescent Protein; I. Introduction; II. Materials; III. Methods; IV. Critical Aspects of the Procedures; V. Results and Discussion; VI. Conclusions and Prospects; References; Chapter 2. Functional Effects of Structural Changes in Photosystem II as Measured by Chlorophyll Fluorescence Kinetics; I. Introduction; II. Materials
  • III. MethodsIV. Parameters Influencing Chlorophyll Fluorescence; V. Fluorescence as a Probe for Photosystem II; VI. Conclusions and Perspectives; References; Chapter 3. Determination of Protein lsoprenylation in Vitro and in Vivo; I. Introduction; II. Analysis of Protein Isoprenylation in Vitro; III. Analysis of Protein Isoprenylation in Vivo; References; Chapter 4. Extraction and Assay of Protein from Single Plant Cells; I. Introduction; II. Preparation of the Sample; III. Extraction and Hydrolysis of the Sample; IV. Analysis of Glutamate in the Protein Hydrolysate
  • V. Advantages and LimitationsReferences; Chapter 5. Photoaffinity Labeling and Strategies for Plasma Membrane Protein Purification; I. Introduction; II. Materials; III. Methods; IV. Results and Discussion; V. Conclusions; References; Chapter 6. Cell Cycle Synchronization, Chromosome Isolation, and Flow-Sorting in Plants; I. Introduction; II. Materials; Ill. Procedure; IV. Critical Aspects of the Procedure; V. Results and Discussion; VI. Conclusions and Perspectives; References; Chapter 7. Principles and Applications of Recombinant Antibody Phage Display Technology to Plant Biology
  • I. IntroductionII. Construction of High-Diversity, Naive Recombinant Antibody Libraries in Bacteriophage fd; III. Panning and Characterization of Recombinant Antibodies from Phage Libraries; IV. Application of Recombinant Antibody Technology to Plant Biology; References; Chapter 8. Isolation and Characterization of Plant Nuclei; I. Introduction; II. Materials; III. Methods; IV. Results and Discussion; V. Conclusions and Perspectives; References; Chapter 9. Isolation of Nuclei Suitable for in Vitro Transcriptional Studies; I. Introduction; II. Isolation of Nuclei
  • III. In Vitro Transcription Run-On AssaysIV. Detection of Labeled Transcripts; V. Critical Aspects of the Procedure; VI. Results and Discussion; VII. Conclusions and Perspectives; References; Chapter 10. Analysis of H+ -ATPase and Other Proteins of the Arabidopsis Plasma Membrane; I. Introduction; II. Growth of Arabidopsis; III. Rapid Extraction Procedures for Protein; IV. Isolation of Membranes; V. Subfractionation of Membranes; VI. Membrane Markers; VII. Conclusions and Perspectives; References; Chapter 11. Isolation and Functional Reconstitution of the Vacuolar H+ -ATPase; I. Introduction

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