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Methods in cell biology. Volume 50, Methods in plant cell biology. Part B /
Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical descriptio...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1995.
|
| Colección: | Methods in cell biology ;
v. 50. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Methods in cell biology. |n Volume 50, |p Methods in plant cell biology. |n Part B / |c edited by David W. Galbraith, Don P. Bourque, Hans J. Bohnert. |
| 246 | 3 | 0 | |a Methods in plant cell biology |
| 260 | |a San Diego : |b Academic Press, |c �1995. | ||
| 300 | |a 1 online resource (xiii, 555 pages) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in cell biology ; |v v. 50 | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 520 | |a Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. This is accompanied by examples of typical results, illustrations, troubleshooting of potential pitfalls, sources of chemicals and equipment, and complete reference lists. Protocols are written to be easily comprehended by beginning research students, but these extensive volumes will also be a valuable addition to the libraries of expert researchers. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants. | ||
| 505 | 0 | |a Front Cover; Methods in Cell Biology: Methods in Plant Cell Biology, Part B; Copyright Page; Contents; Contributors; Preface; Part I: Subcellular Fractionation and Analysis of Function; Chapter 1. Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green Fluorescent Protein; I. Introduction; II. Materials; III. Methods; IV. Critical Aspects of the Procedures; V. Results and Discussion; VI. Conclusions and Prospects; References; Chapter 2. Functional Effects of Structural Changes in Photosystem II as Measured by Chlorophyll Fluorescence Kinetics; I. Introduction; II. Materials | |
| 505 | 8 | |a III. MethodsIV. Parameters Influencing Chlorophyll Fluorescence; V. Fluorescence as a Probe for Photosystem II; VI. Conclusions and Perspectives; References; Chapter 3. Determination of Protein lsoprenylation in Vitro and in Vivo; I. Introduction; II. Analysis of Protein Isoprenylation in Vitro; III. Analysis of Protein Isoprenylation in Vivo; References; Chapter 4. Extraction and Assay of Protein from Single Plant Cells; I. Introduction; II. Preparation of the Sample; III. Extraction and Hydrolysis of the Sample; IV. Analysis of Glutamate in the Protein Hydrolysate | |
| 505 | 8 | |a V. Advantages and LimitationsReferences; Chapter 5. Photoaffinity Labeling and Strategies for Plasma Membrane Protein Purification; I. Introduction; II. Materials; III. Methods; IV. Results and Discussion; V. Conclusions; References; Chapter 6. Cell Cycle Synchronization, Chromosome Isolation, and Flow-Sorting in Plants; I. Introduction; II. Materials; Ill. Procedure; IV. Critical Aspects of the Procedure; V. Results and Discussion; VI. Conclusions and Perspectives; References; Chapter 7. Principles and Applications of Recombinant Antibody Phage Display Technology to Plant Biology | |
| 505 | 8 | |a I. IntroductionII. Construction of High-Diversity, Naive Recombinant Antibody Libraries in Bacteriophage fd; III. Panning and Characterization of Recombinant Antibodies from Phage Libraries; IV. Application of Recombinant Antibody Technology to Plant Biology; References; Chapter 8. Isolation and Characterization of Plant Nuclei; I. Introduction; II. Materials; III. Methods; IV. Results and Discussion; V. Conclusions and Perspectives; References; Chapter 9. Isolation of Nuclei Suitable for in Vitro Transcriptional Studies; I. Introduction; II. Isolation of Nuclei | |
| 505 | 8 | |a III. In Vitro Transcription Run-On AssaysIV. Detection of Labeled Transcripts; V. Critical Aspects of the Procedure; VI. Results and Discussion; VII. Conclusions and Perspectives; References; Chapter 10. Analysis of H+ -ATPase and Other Proteins of the Arabidopsis Plasma Membrane; I. Introduction; II. Growth of Arabidopsis; III. Rapid Extraction Procedures for Protein; IV. Isolation of Membranes; V. Subfractionation of Membranes; VI. Membrane Markers; VII. Conclusions and Perspectives; References; Chapter 11. Isolation and Functional Reconstitution of the Vacuolar H+ -ATPase; I. Introduction | |
| 546 | |a English. | ||
| 650 | 0 | |a Cytology |x Technique. | |
| 650 | 0 | |a Plant cells and tissues. | |
| 650 | 0 | |a Cells. | |
| 650 | 0 | |a Biology. | |
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| 650 | 6 | |a Plantes |x Cellules et tissus. |0 (CaQQLa)201-0019072 | |
| 650 | 6 | |a Plantes. |0 (CaQQLa)201-0000153 | |
| 650 | 6 | |a Cellules. |0 (CaQQLa)201-0005079 | |
| 650 | 6 | |a Biologie. |0 (CaQQLa)201-0000137 | |
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| 650 | 7 | |a Cytology |x Technique |2 fast |0 (OCoLC)fst00886306 | |
| 650 | 7 | |a Plant cells and tissues |2 fast |0 (OCoLC)fst01065318 | |
| 653 | 0 | |a Plants |a Cells | |
| 700 | 1 | |a Galbraith, David W. | |
| 700 | 1 | |a Bohnert, Hans J. | |
| 700 | 1 | |a Bourque, Don P. | |
| 776 | |z 0-12-564152-4 | ||
| 830 | 0 | |a Methods in cell biology ; |v v. 50. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780125641524 |z Texto completo |