Methods in cell biology. Volume 17, Chromatin and chromosomal protein research. II /

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METHODS IN CELL BIOLOGY,VOLUME 17: CHROMATIN AND CHROMOSOMAL PROTEIN RESEARCH II.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Stein, Gary, Stein, Janet, Kleinsmith, Lewis J.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Academic Press, 1978.
Colección:Methods in cell biology ; v. 17.
Temas:
Cytology > Technique.
Biology > Technique.
Cytologie > Technique.
Biologie > Technique.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover; Methods in Cell Biology, Volume XVII; Copyright Page; Contents; List of Contributers; Preface; Erratum; Part A: Isolation of Nuclei and Preparation of Chromatin. II; Chapter I. Procedures for Minimizing Protease Activity during Isolation of Nuclei, Chromatin, and the Histones; I. Introduction; II. Methods; III. Conditions and Patterns of Proteolytic Degradation of Histones; IV. Concluding Remarks; References; Chapter 2. Isolation of Nuclei from Animal Cells in Culture; I. Introduction; II. General Consideration of Nuclear Isolation Procedures; III. Methods for Isolating Nuclei
  • II. Method A: For Studies on Lysine-Rich and Slightly Lysine-Rich HistonesIII. Method B: For Studies on Arginine-Rich Histones; References; Chapter 5. Methods for Isolation of Nuclei from Spermatozoa; I. Introduction; II. Spermatozoa of Eutherian Mammals; III. Spermatozoa with Protamine; IV. Spermatozoa with Somatic Histone-Type Protein; V. Spermatozoa of Molluscs; VI. Spermatozoa of Cricket; VII. Spermatozoa of Crab; References; Chapter 6. Manual Enucleation of Xenopus Oocytes; I. Introduction; II. Methods; III. Conclusions; References
  • Chapter 7. Macro and Micro Methods for Isolation and Injection of Cell Components from Polytene TissuesI. Mass Isolation of Salivary Glands from Drosophila hydei; II. Micromanipulation of Salivary Glands; III. Microinjection of Salivary Gland Cells; References; Chapter 8. Isolation of Nuclei and Chromatin from Phycomyces blakesleeanus; I. Introduction; II. Methods; References; Part B: Chromosomes; Chapter 9. Mammalian Metaphase Chromosomes; I. Introduction; II. General Procedures; III. Isolation of Chromosomes; IV. Chromosome Aggregation; References
  • Chapter 10. Analytical Techniques for Isolated Metaphase Chromosome FractionsI. Introduction; II. Chromosome Isolation; III. Fractionation of Isolated Chromosomes; IV. Fraction Analysis; V. Computer Analysis; References; Chapter 11. Methods and Mechanisms of Chromosome Banding; I. Introduction; II. Slide Preparation; III. Q-Banding; IV. G-Banding; V. C-Banding; VI. R-Banding; VII. BG-Banding; References; Chapter 12. Cytological Analysis of Drosophila Polytene Chromosomes; I. Introduction; II. Staining with Aceto-orcein and Fast Green FCF; III. Staining with Naphtol-Yellow S (NYS)

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