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Flow cytogenetics /
This is the first book to be devoted entirely to the application and development of flow techniques in cytogenetics. It provides comprehensive information on the use of flow cytometry and sorting for chromosome classification and purification. Cytogenetics and molecular biologists will find this boo...
| Clasificación: | Libro Electrónico |
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| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London ; San Diego :
Academic Press,
�1989.
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| Colección: | Analytical cytology series.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Flow Cytogenetics; Copyright Page; Table of Contents; Contributors; Preface; Chapter 1. Human Chromosome Analysis by Flow Cytometry; I. Introduction; II. Factors Affecting Resolution; III. Application of Flow Systems to Karyotype Analysis; IV. Flow Sorting; V. Applications of Chromosome Sorting; VI. Future Prospects; Acknowledgements; References; Chapter 2. Instrumentation for Chromosome Analysis and Sorting; I. Introduction; II. Measurement Precision; III. Fluidics; IV. Electronics; V. Data Acquisition; VI. Data Display; VII. Chromosome Sorting; VIII. Future Directions
- AcknowledgementsReferences; Chapter 3. Cell Culture for Chromosome Isolation; I. Introduction; II. Culture Environment; III. Mitotic Cell Accumulation; IV. Specific Examples; V. Summary; Acknowledgements; References; Chapter 4. Chromosome Isolation Procedures; I. Introduction; II. Preparation Details; III. Characteristics of Isolated Chromosomes; IV. Summary and Recommendations; Acknowledgements; References; Chapter 5. DNA Stains as Cytochemical Probes for Chromosomes; I. Introduction; II. Principles of Chromosome Staining; III. Stain Characteristics
- IV. Characteristics of Specific Staining MethodsV. Mechanisms of Chromosome Staining and Banding; VI. Future Directions; Acknowledgements; References; Chapter 6. Methods for Estimating Components of Multipeaked Flow Histograms; I. Introduction; II. Mathematical Description of the Problem; III. Univariate Methods; IV. Comparison of Univariate Methods; V. Bivariate Methods; VI. Application of Bivariate Methods to Flow Chromosome Data; VII. Current Approaches in Flow Cytometry; VIII. Future Directions; Acknowledgements; References; Chapter 7. Univariate Flow Karyotype Analysis; I. Introduction
- II. Techniques for High-Resolution Univariate Flow Karyotype AnalysisIII. Data Interpretation; IV. Univariate Chromosome Sorting; V. Specific Biological Applications of Univariate Flow Karyotype Analysis; VI. Summary and Future Directions; Acknowledgements; References; Chapter 8. Bivariate Flow Karyotyping; I. Introduction; II. Principles of Bivariate Flow Karyotyping; III. Chromosome Aberration Detection; IV. Hybrid Cell Characterization; V. Chromosome Sorting; VI. Future Directions; Acknowledgements; References
- Chapter 9. Relation Between Radiation-induced Flow Karyotype Changes Analysed by Fourier Analysis and Chromosome AberrationsI. Introduction; II. Radiation Induced Changes in Flow Karyotypes; III. Application of Discrete Fourier Analysis to Flow Karyotypes; IV. Chromosome Damage Observed in Flow Karyotypes in Relation to Asymmetrical Chromosome Aberrations; Acknowledgements; References; Chapter 10. Detection of Randomly Occurring Aberrant Chromosomes as a Measure of Genetic Change; I. Unstable Chromosome Aberration Scoring; II. Single-Fluorochrome Flow Karyotypes