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Heterotrimeric G proteins /
This volume of Methods in Enzymology and its companion Volume 238 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins (Volume 237) and their effectors (Volume 238). Methods unique to signal transducing G proteins and general techniques appli...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1994.
|
| Colección: | Methods in enzymology ;
v. 237. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo |
Tabla de Contenidos:
- Measurement of receptor-stimulated guanosine 5�a#x80;²-O-(�I³-thio)triphosphate binding by G proteins / Thomas Wieland, Karl H Jakobs
- Receptor-stimulated hydrolysis of guanosine 5�a#x80;²-triphosphate in membrane preparations / Peter Gierschik, Thomas Bouillon, Karl H Jakobs
- Regulation of G-protein activation by mastoparans and other cationic peptides / Elliott M Ross, Tsutomu Higashuima
- Guanosine 5�a#x80;²-O-(�I³-thio)triphosphate binding assay for solubilized G proteins / Donna J Carty, Ravi Iyengar
- Activation of cholera toxin by ADP-ribosylation factors: 20-kDa guanine nucleotide-binding proteins / Joel Moss, Randy S Haun, Su-Chen Tsai, Catherine F Welsh, Fang-Jen Scott Lee, S Russ Price, Martha Vaughan
- Pertussis toxin-catalyzed ADP-ribosylation of G proteins / Donna J Carty
- Synthesis and use of radioactive photoactivatable NAD+ derivatives as probes for G-protein structure / Richard R Vaillancourt, N Dhanasekaran, Arnold E Ruoho
- Photoaffinity guanosine 5�a#x80;²-triphosphate analogs as a tool for the study of GTP-binding proteins / Mark M Rasenick, Madhavi Talluri, William J Dunn III
- Preparation of activated �I� subunits of Gs and GiS: From erythrocyte to activated subunit / Lutz Birnbaumer, Dagoberto Grenet, Fernando Ribeiro-Neto, Juan Codina
- Purification and separation of closely related members of pertussis toxin-substrate G proteins / Toshiaki Katada, Kenji Kontani, Atsushi Inanobe, Ichiro Kobayashi, Yoshiharu Ohoka, Hiroshi Nishina, Katsunobu Takahashi
- Purification of transducin / Joe�I#x88;lle Bigay, Marc Chabre
- Expression of G-protein a subunits in Escherichia coli / Ethan Lee, Maurine E Linder, Alfred G Gilman
- Synthesis and applications of affinity matrix containing immobilized �I²�I³ subunits of G proteins / Iok-Hou Pang, Alan V Smrcka, Paul C Sternweis
- Purification of activated and heterotrimeric forms of Gq proteins / Jonathan L Blank, John H Exton
- Purification of Phospholipase C-activating G protein, G11, from Turkey erythrocytes / Gary L Waldo, Jose�I#x81; L Boyer, T Kendall Harden
- Purification of recombinant Gq�I�, G11�I�, and G16�I� from Sf9 cells / John R Hepler, Tohru Kozasa, Alfred G Gilman
- Expression and purification of G-protein �I� subunits using baculovirus expression system / Stephen G Graber, Robert A Figler, James C Garrison
- Analysis of G-protein �I� and �I²�I³ subunits by in vitro translation / Eva J Neer, Bradley M Denker, Thomas C Thomas, Carl J Schmidt
- Assays for studying functional properties of in vitro translated Gs�I� subunit / Yves Audigier
- Myristoylation of G-protein �I� subunits / Susanne M Mumby, Maurine E Linder
- Specificity and functional applications of antipeptide antisera which identify G-protein �I� subunits / Graeme Milligan
- Identification of receptor-activated G proteins: Selective immunoprecipitation of photolabeled G-protein �I� subunits / Karl-Ludwig Laugwitz, Karsten Spicher, Gu�I#x88;nter Schultz, Stefan Offermanns
- Identification of mutant forms of G-protein �I� subunits in human neoplasia by polymerase chain reaction-based techniques / John Lyons
- Detection of mutations and polymorphisms of Gs�I� subunit gene by denaturing gradient Gel electrophoresis / Pablo V Gejman, Lee S Weinstein
- Construction of mutant and chimeric G-protein �I� subunits / Sim Winitz, Marijane Russell, Gary L Johnson
- Design of degenerate oligonucleotide primers for cloning of G-protein a subunits / Thomas M Wilkie, Anna M Aragay, A John Watson, Melvin I Simon
- Microinjection of antisense oligonucleotides to assess G-protein subunit function / Christiane Kleuss, Gu�I#x88;nter Schultz, Burghardt Wittig
- Inactivation of G-protein genes: Double knockout in cell lines / Richard M Mortensen, J.G Seidman
- Targeted Inactivation of the G12�I� gene with replacement and insertion vectors: Analysis in a 96-well plate format / Uwe Rudolph, Allan Bradley, Lutz Birnbaumer
- G-Protein assays in Dictyostelium / B Ewa Snaar-Jagalska, Peter J.M Van Haastert
- Fluorescence assays for G-protein interactions / Richard A Cerione
- Specific peptide probes for G-protein interactions with receptors / Heidi E Hamm, Helen M Rarick
- Vaccinia virus systems for expression of G�I� genes in S49 cells / Franklin Quan, Michael Forte
- Purification of T�I²�I³ subunit of transducin / Joe�I#x88;lle Bigay, Marc Chabre
- Adenylyl cyclase assay for �I²�I³ subunits of G proteins / Jianqiang Chen, Donna J Carty, Ravi Iyengar
- Synthesis and use of biotinylated �I²�I³ complexes prepared from bovine brain G proteins / Jane Dingus, Michael D Wilcox, Russell Kohnken, John D Hildebrandt
- Design of oligonucleotide probes for molecular cloning of �I² and �I³ subunits / Christine Gallagher, Narasimhan Gautam
- Characterization of antibodies for various G-protein �I² and �I³ subunits / Alexey N Pronin, Narasimhan Gautam
- Preparation, characterization, and use of antibodies with specificity for G-protein y subunits / Janet D Robishaw, Eric A Balcueva
- Isoprenylation of �I³ subunits and G-protein effectors / Bernard K.-K Fung, Janmeet S Anant, Wun-Chen Lin, Olivia C Ong, Harvey K Yamane.