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Protein engineering /
This MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, California :
Elsevier Academic Press,
�2004.
|
| Colección: | Methods in enzymology ;
v. 388. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo Texto completo Texto completo |
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| 245 | 0 | 0 | |a Protein engineering / |c edited by Dan E. Robertson, Joseph P. Noel. |
| 260 | |a San Diego, California : |b Elsevier Academic Press, |c �2004. | ||
| 300 | |a 1 online resource (xxxvii, 413 pages, 16 unnumbered pages of plates) : |b illustrations (some color). | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
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| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v v. 388 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 505 | 0 | |a Section I. Methodology -- Gene site saturation mutagenesis : a comprehensive mutagenesis approach -- Single-stranded DNA family shuffling -- Module shuffling -- SCHEMA-guided protein recombination -- Staggered extension process in vitro DNA recombination -- Using incremental truncation to create libraries of hybrid enzymes -- Random multirecombinant polymerase chain reaction -- Gene library synthesis by structure-based combinatorial protein engineering -- New enzymes from combinatorial library modules -- Construction of protein fragment complementation libraries using incremental truncation -- Section II. Applications: optimization and screening -- GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform -- High throughput microplate screens for directed protein evolution -- Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities -- Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis -- Screening for oxidative resistance -- Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes -- Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system -- Section III. Applications: directed evolution of enzymatic function -- Directed evolution of lipases and esterases -- Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium -- Directed evolution of aldolases -- Changing the enantioselectivity of enzymes by directed evolution -- Control of stereoselectivity in phosphotriesterase -- Section IV. Applications: evolution of biosynthetic pathways -- Manipulation and analysis of polyketide synthases -- Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases -- Engineering carotenoid biosynthetic pathways -- Section V. Devices, antibodies, and vaccines -- Directed evolution of bacteriorhodopsin for device applications -- Engineering antibody affinity by yeast surface display -- A conditionally replicating virus as a novel approach toward an HIV vaccine. | |
| 588 | 0 | |a Print version record. | |
| 520 | |a This MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies. | ||
| 650 | 0 | |a Protein engineering. | |
| 650 | 0 | |a Proteins |x Synthesis. | |
| 650 | 0 | |a Genetic engineering. | |
| 650 | 0 | |a Proteins |x Structure-activity relationships. | |
| 650 | 0 | |a Proteins |x Conformation. | |
| 650 | 2 | |a Genetic Engineering |0 (DNLM)D005818 | |
| 650 | 2 | |a Protein Conformation |0 (DNLM)D011487 | |
| 650 | 2 | |a Proteins |x genetics |0 (DNLM)D011506Q000235 | |
| 650 | 2 | |a Protein Engineering |0 (DNLM)D015202 | |
| 650 | 2 | |a Protein Biosynthesis |0 (DNLM)D014176 | |
| 650 | 6 | |a Technologie des prot�eines. |0 (CaQQLa)201-0161338 | |
| 650 | 6 | |a Prot�eines |x Synth�ese. |0 (CaQQLa)201-0031403 | |
| 650 | 6 | |a Prot�eines |0 (CaQQLa)201-0008150 |x Relations structure-activit�e. |0 (CaQQLa)201-0376206 | |
| 650 | 6 | |a G�enie g�en�etique. |0 (CaQQLa)201-0022662 | |
| 650 | 6 | |a Prot�eines |x Conformation. |0 (CaQQLa)201-0217301 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Proteins |x Conformation |2 fast |0 (OCoLC)fst01079725 | |
| 650 | 7 | |a Genetic engineering |2 fast |0 (OCoLC)fst00940027 | |
| 650 | 7 | |a Protein engineering |2 fast |0 (OCoLC)fst01079685 | |
| 650 | 7 | |a Proteins |x Structure-activity relationships |2 fast |0 (OCoLC)fst01079762 | |
| 650 | 7 | |a Proteins |x Synthesis |2 fast |0 (OCoLC)fst01079764 | |
| 650 | 7 | |a Proteine |2 gnd |0 (DE-588)4076388-2 | |
| 650 | 7 | |a Enzymologie |2 gnd |0 (DE-588)4152482-2 | |
| 650 | 1 | 7 | |a DNA. |2 gtt |
| 650 | 1 | 7 | |a Recombinatie. |2 gtt |
| 650 | 1 | 7 | |a Eiwitsynthese. |2 gtt |
| 650 | 7 | |a Ing�enierie des prot�eines. |2 rasuqam | |
| 650 | 7 | |a Synth�ese prot�eique. |2 rasuqam | |
| 650 | 7 | |a Manipulation g�en�etique. |2 rasuqam | |
| 650 | 7 | |a Relation structure-activit�e (Biochimie) |2 rasuqam | |
| 650 | 7 | |a Prot�eine. |2 rasuqam | |
| 655 | 7 | |a Aufsatzsammlung. |0 (DE-588)4143413-4. |2 gnd | |
| 700 | 1 | |a Robertson, Dan E., |e editor. | |
| 700 | 1 | |a Noel, Joseph P., |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Protein engineering. |d San Diego, California : Elsevier Academic Press, �2004 |z 0121827933 |w (OCoLC)56137371 |
| 830 | 0 | |a Methods in enzymology ; |v v. 388. |x 0076-6879 | |
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| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/388 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/388 |z Texto completo |
| 856 | 4 | 1 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879 |z Texto completo |
| 856 | 4 | 1 | |u https://sciencedirect.uam.elogim.com/science/journal/00766879 |z Texto completo |