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The Zebrafish : genetics and genomics /

This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features* Details state-of-the-art zebrafis...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor Corporativo: American Society for Cell Biology
Otros Autores: Detrich, H. William, III, Westerfield, Monte, Zon, Leonard I.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1999.
Colección:Methods in cell biology ; v. 60.
Temas:
Acceso en línea:Texto completo
Texto completo

MARC

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245 0 4 |a The Zebrafish :  |b genetics and genomics /  |c edited by H. William Detrich, III, Monte Westerfield, Leonard I. Zon. 
260 |a San Diego :  |b Academic Press,  |c �1999. 
300 |a 1 online resource (xviii, 396 pages, 11 unnumbered pages of plates) :  |b illustrations (some color) 
336 |a text  |b txt  |2 rdacontent 
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490 1 |a Methods in cell biology ;  |v v. 60 
504 |a Includes bibliographical references and index. 
520 |a This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features* Details state-of-the-art zebrafish protocols in a single-source reference* Presents methods and reagents in user-friendly format* Delineates critical steps and pitfalls of he procedures* Illustrates techniques with full-color plates* Summarizes many new and interesting developmental mutants* Includes appendices with strain informati. 
588 0 |a Print version record. 
505 0 |a Front Cover; Methods in Cell Biology, Volume 60; Copyright Page; Contents; Contributors; Preface; Chapter 1. A Gynogenesis-Based Screen for Maternal-Effect Genes in the Zebrafish, Danio rerio; I. Introduction; II. Optimization of the Gynogenetic Method; III. A Pilot Screen for Maternal-Effect Mutations; IV. Recovery of Mutations; V. Solutions, Materials, and Protocols for Gynogenetic Methods; VI. Conclusions; References; Chapter 2. Developmental Mutant Screens in the Zebrafish; I. Introduction; II. Mutagenesis; III. Breeding Scheme; IV. Screening Procedure; V. Allele Screens 
505 8 |a VI. Dominant ScreensVII. Managing the Outcome of Screens; VIII. Potential of Future Screens; Appendix-Recipes and Media; References; Chapter 3. Haploid Screens and Gamma-Ray Mutagenesis; I. Introduction; II. Production of Haploid Embryos; III. Development of Haploid Embryos; IV. Genetic Background; V. The Haploid Screen; VI. Limitations of Haploids; VII. Mosaicism in F1 Screens; VIII. Haploid Screens and Gamma-Ray Mutagenesis; IX. Nature of Gamma-Ray-Induced Mutations; X. Conclusion; References; Chapter 4. Early Pressure Screens; I. Introduction 
505 8 |a II. Rationale for Use of Early Pressure to Produce Diploid Embryos for Genetic ScreensIII. Rationale for Use of Screens Focused on Specific Phenotypes; IV. Design of Our Screen and Mutant Phenotypes Isolated; V. Closing Remarks; References; Chapter 5. Retrovirus-Mediated Insertional Mutagenesis in Zebrafish; I. Introduction; II. Insertional Mutagenesis: A Pilot Screen; III. Considerations for a Large-Scale Screen; References; Chapter 6. Genetic Applications of Transposons and Other Repetitive Elements in Zebrafish; I. Introduction; II. Classification of Repetitive Elements 
505 8 |a III. Genetic Applications of Repetitive ElementsIV. Summary and Perspectives; References; Chapter 7. Transgenesis; I. Introduction; II. Materials; III. Methods; IV. Discussion; References; Chapter 8. The Zebrafish Genome; I. Introduction; II. Mapping the Zebrafish Genome; III. The Zebrafish Gene Map; IV. Two Genes in Zebrafish for One in Mammals; V. Gene Nomenclature in Zebrafish; VI. Conclusions; References; Chapter 9. Using Random Amplified Polymorphic DNAs in Zebrafish Genomic Analysis; I. Introduction; II. What Are RAPDs?; III. Advantages and Disadvantages of RAPDs as Polymorphic Markers 
505 8 |a IV. Methods for Developing RAPD MarkersV. Using RAPDs to Identify Markers Closely Linked to a Mutation; VI. Conversion of RAPD Markers to Sequence Tagged Sites; VII. Conclusions; References; Chapter 10. Simple Sequence-Length Polymorphism Analysis; I. Analysis in DNA Sequencing Gels; II. Alternative Analysis of SSLP-Containing PCR Product in High-Resolution Agarose Gels; III. Troubleshooting and Other Considerations; References; Chapter 11. Gene Mapping in Zebrafish Using Single-Strand Conformation Polymorphism Analysis; I. Introduction; II. SSCP Methodology; III. Gene Mapping; IV. Methods 
546 |a English. 
650 0 |a Zebra danio  |x Genetics. 
650 0 |a Zebra danios as laboratory animals. 
650 0 |a Genomes. 
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650 2 2 |a Chromosome Mapping  |x methods  |0 (DNLM)D002874Q000379 
650 2 2 |a Genome  |0 (DNLM)D016678 
650 2 2 |a Mutagenesis  |0 (DNLM)D016296 
650 2 2 |a Mutation  |x genetics  |0 (DNLM)D009154Q000235 
650 6 |a Danio z�ebr�e  |0 (CaQQLa)201-0322736  |x G�en�etique.  |0 (CaQQLa)201-0380134 
650 6 |a Danio z�ebr�e (Animal de laboratoire)  |0 (CaQQLa)201-0322790 
650 6 |a G�enomes.  |0 (CaQQLa)201-0071616 
650 7 |a SCIENCE  |x Life Sciences  |x Zoology  |x Ichthyology & Herpetology.  |2 bisacsh 
650 7 |a NATURE  |x Animals  |x Fish.  |2 bisacsh 
650 7 |a Poissons.  |2 eclas 
650 7 |a Biologie marine.  |2 eclas 
650 7 |a Biologie mol�eculaire.  |2 eclas 
650 7 |a Genomes.  |2 fast  |0 (OCoLC)fst00940225 
650 7 |a Zebra danio  |x Genetics.  |2 fast  |0 (OCoLC)fst01184145 
650 7 |a Zebra danios as laboratory animals.  |2 fast  |0 (OCoLC)fst01184146 
700 1 |a Detrich, H. William,  |c III. 
700 1 |a Westerfield, Monte. 
700 1 |a Zon, Leonard I. 
710 2 |a American Society for Cell Biology. 
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830 0 |a Methods in cell biology ;  |v v. 60. 
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