The Zebrafish : genetics and genomics /
This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features* Details state-of-the-art zebrafis...
Clasificación: | Libro Electrónico |
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Autor Corporativo: | |
Otros Autores: | , , |
Formato: | Electrónico eBook |
Idioma: | Inglés |
Publicado: |
San Diego :
Academic Press,
�1999.
|
Colección: | Methods in cell biology ;
v. 60. |
Temas: | |
Acceso en línea: | Texto completo Texto completo |
MARC
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050 | 4 | |a QH585 |b .M47eb vol. 60 | |
055 | 3 | |a QH585 |b .M47 v.60 | |
060 | 4 | |a W1 |b ME9615D v.60 1999 | |
060 | 4 | |a QL 638.C94 |b Z42 1999 | |
070 | |a 442 |b P92 v.60 | ||
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245 | 0 | 4 | |a The Zebrafish : |b genetics and genomics / |c edited by H. William Detrich, III, Monte Westerfield, Leonard I. Zon. |
260 | |a San Diego : |b Academic Press, |c �1999. | ||
300 | |a 1 online resource (xviii, 396 pages, 11 unnumbered pages of plates) : |b illustrations (some color) | ||
336 | |a text |b txt |2 rdacontent | ||
337 | |a computer |b c |2 rdamedia | ||
338 | |a online resource |b cr |2 rdacarrier | ||
490 | 1 | |a Methods in cell biology ; |v v. 60 | |
504 | |a Includes bibliographical references and index. | ||
520 | |a This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features* Details state-of-the-art zebrafish protocols in a single-source reference* Presents methods and reagents in user-friendly format* Delineates critical steps and pitfalls of he procedures* Illustrates techniques with full-color plates* Summarizes many new and interesting developmental mutants* Includes appendices with strain informati. | ||
588 | 0 | |a Print version record. | |
505 | 0 | |a Front Cover; Methods in Cell Biology, Volume 60; Copyright Page; Contents; Contributors; Preface; Chapter 1. A Gynogenesis-Based Screen for Maternal-Effect Genes in the Zebrafish, Danio rerio; I. Introduction; II. Optimization of the Gynogenetic Method; III. A Pilot Screen for Maternal-Effect Mutations; IV. Recovery of Mutations; V. Solutions, Materials, and Protocols for Gynogenetic Methods; VI. Conclusions; References; Chapter 2. Developmental Mutant Screens in the Zebrafish; I. Introduction; II. Mutagenesis; III. Breeding Scheme; IV. Screening Procedure; V. Allele Screens | |
505 | 8 | |a VI. Dominant ScreensVII. Managing the Outcome of Screens; VIII. Potential of Future Screens; Appendix-Recipes and Media; References; Chapter 3. Haploid Screens and Gamma-Ray Mutagenesis; I. Introduction; II. Production of Haploid Embryos; III. Development of Haploid Embryos; IV. Genetic Background; V. The Haploid Screen; VI. Limitations of Haploids; VII. Mosaicism in F1 Screens; VIII. Haploid Screens and Gamma-Ray Mutagenesis; IX. Nature of Gamma-Ray-Induced Mutations; X. Conclusion; References; Chapter 4. Early Pressure Screens; I. Introduction | |
505 | 8 | |a II. Rationale for Use of Early Pressure to Produce Diploid Embryos for Genetic ScreensIII. Rationale for Use of Screens Focused on Specific Phenotypes; IV. Design of Our Screen and Mutant Phenotypes Isolated; V. Closing Remarks; References; Chapter 5. Retrovirus-Mediated Insertional Mutagenesis in Zebrafish; I. Introduction; II. Insertional Mutagenesis: A Pilot Screen; III. Considerations for a Large-Scale Screen; References; Chapter 6. Genetic Applications of Transposons and Other Repetitive Elements in Zebrafish; I. Introduction; II. Classification of Repetitive Elements | |
505 | 8 | |a III. Genetic Applications of Repetitive ElementsIV. Summary and Perspectives; References; Chapter 7. Transgenesis; I. Introduction; II. Materials; III. Methods; IV. Discussion; References; Chapter 8. The Zebrafish Genome; I. Introduction; II. Mapping the Zebrafish Genome; III. The Zebrafish Gene Map; IV. Two Genes in Zebrafish for One in Mammals; V. Gene Nomenclature in Zebrafish; VI. Conclusions; References; Chapter 9. Using Random Amplified Polymorphic DNAs in Zebrafish Genomic Analysis; I. Introduction; II. What Are RAPDs?; III. Advantages and Disadvantages of RAPDs as Polymorphic Markers | |
505 | 8 | |a IV. Methods for Developing RAPD MarkersV. Using RAPDs to Identify Markers Closely Linked to a Mutation; VI. Conversion of RAPD Markers to Sequence Tagged Sites; VII. Conclusions; References; Chapter 10. Simple Sequence-Length Polymorphism Analysis; I. Analysis in DNA Sequencing Gels; II. Alternative Analysis of SSLP-Containing PCR Product in High-Resolution Agarose Gels; III. Troubleshooting and Other Considerations; References; Chapter 11. Gene Mapping in Zebrafish Using Single-Strand Conformation Polymorphism Analysis; I. Introduction; II. SSCP Methodology; III. Gene Mapping; IV. Methods | |
546 | |a English. | ||
650 | 0 | |a Zebra danio |x Genetics. | |
650 | 0 | |a Zebra danios as laboratory animals. | |
650 | 0 | |a Genomes. | |
650 | 1 | 2 | |a Zebrafish |x genetics |0 (DNLM)D015027Q000235 |
650 | 2 | 2 | |a Chromosome Mapping |x methods |0 (DNLM)D002874Q000379 |
650 | 2 | 2 | |a Genome |0 (DNLM)D016678 |
650 | 2 | 2 | |a Mutagenesis |0 (DNLM)D016296 |
650 | 2 | 2 | |a Mutation |x genetics |0 (DNLM)D009154Q000235 |
650 | 6 | |a Danio z�ebr�e |0 (CaQQLa)201-0322736 |x G�en�etique. |0 (CaQQLa)201-0380134 | |
650 | 6 | |a Danio z�ebr�e (Animal de laboratoire) |0 (CaQQLa)201-0322790 | |
650 | 6 | |a G�enomes. |0 (CaQQLa)201-0071616 | |
650 | 7 | |a SCIENCE |x Life Sciences |x Zoology |x Ichthyology & Herpetology. |2 bisacsh | |
650 | 7 | |a NATURE |x Animals |x Fish. |2 bisacsh | |
650 | 7 | |a Poissons. |2 eclas | |
650 | 7 | |a Biologie marine. |2 eclas | |
650 | 7 | |a Biologie mol�eculaire. |2 eclas | |
650 | 7 | |a Genomes. |2 fast |0 (OCoLC)fst00940225 | |
650 | 7 | |a Zebra danio |x Genetics. |2 fast |0 (OCoLC)fst01184145 | |
650 | 7 | |a Zebra danios as laboratory animals. |2 fast |0 (OCoLC)fst01184146 | |
700 | 1 | |a Detrich, H. William, |c III. | |
700 | 1 | |a Westerfield, Monte. | |
700 | 1 | |a Zon, Leonard I. | |
710 | 2 | |a American Society for Cell Biology. | |
776 | 0 | 8 | |i Print version: |t Zebrafish. |d San Diego : Academic Press, �1999 |z 9780125441629 |z 0125441622 |w (OCoLC)40466235 |
830 | 0 | |a Methods in cell biology ; |v v. 60. | |
856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780125441629 |z Texto completo |
856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/60 |z Texto completo |