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Caenorhabditis elegans : modern biological analysis of an organism /

The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four prima...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor Corporativo: American Society for Cell Biology
Otros Autores: Epstein, Henry F., 1944- (Editor ), Shakes, Diane C. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, [1995]
Colección:Methods in cell biology ; v. 48.
Temas:
Acceso en línea:Texto completo
Texto completo
Tabla de Contenidos:
  • pt. 1. Genetic and culture methods. Basic culture methods / James A. Lewis, John T. Fleming
  • Mutagenesis / Philip Anderson
  • Reverse genetics : from gene sequence to mutant worm / Ronald H.A. Plasterk
  • Genetic mapping with polymorphic sequence-tagged sites / Benjamin D. Williams
  • Genetic dissection of developmental pathways / Linda S. Huang, Paul W. Sternberg
  • Mosaic analysis / Robert K. Herman
  • Genetic balancers / Mark L. Edgley [and others]
  • pt 2. Neurobiology. Genetic pharmacology : interactions between drugs and gene products in Caenorhabditis elegans / James B. Rand, Carl D. Johnson
  • Methods of studying behavioral plasticity in Caenorhabditis elegans / Timothy N. Gannon, Catharine H. Rankin
  • Laser killing of cells in Caenorhabditis elegans / Cornelia I. Bargmann, Leon Avery
  • Electrophysiological methods / Leon Avery, David Raizen, Shawn Lockery
  • pt. 3. Cell biology and molecular biology. Cell biology of nematode sperm / Steven W. L'Hernault, Thomas M. Roberts
  • Blastomere culture and analysis / Lois G. Edgar
  • Whole-mount in situ hybridization for the detection of RNA in Caenorhabditis elegans embryos / Geraldine Seydoux, Andrew Fire
  • Fluorescence in situ hybridization for the detection of DNA and RNA / Donna G. Albertson, Rita M. Fishpool, Philip S. Birchall
  • Immunofluorescence microscopy / David M. Miller, Diane C. Shakes
  • Electron microscopy and three-dimensional image reconstruction / David H. Hall.
  • Proteins and protein assemblies / Henry F. Epstein, Feizhou Liu
  • DNA transformation / Craig Mello, Andrew Fire
  • Transcription and translation ; Techniques for analyzing transcription and translation / Michael Krause
  • pt. 4. Genomics and informatics. The physical map of the Caenorhabditis elegans genome / Alan Coulson [and others]
  • Genomic DNA sequencing methods / Anthony Favello, LaDeana Hillier, Richard K. Wilson
  • Large-scale complementary DNA sequencing methods / Lucinda L. Fulton, LaDeana Hillier, Richard K. Wilson
  • ACeDB and Macace / Frank H. Eeckman, Richard Durbin
  • The Worm Community System, release 2.0 (WCSr2) / Laura M. Shoman [and others]
  • Appendix A. Additional information and resources
  • Appendix B. Caenorhabditis Genetics Center, laboratory designations
  • Appendix C. Gene names and descriptions.