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Caenorhabditis elegans : modern biological analysis of an organism /

The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four prima...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor Corporativo: American Society for Cell Biology
Otros Autores: Epstein, Henry F., 1944- (Editor ), Shakes, Diane C. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, [1995]
Colección:Methods in cell biology ; v. 48.
Temas:
Acceso en línea:Texto completo
Texto completo

MARC

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245 0 0 |a Caenorhabditis elegans :  |b modern biological analysis of an organism /  |c edited by Henry F. Epstein and Diane C. Shakes. 
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490 1 |a Methods in cell biology,  |x 0091-679X ;  |v v. 48 
500 |a "Prepared under the auspices of the American Society for Cell Biology." 
504 |a Includes bibliographical references and index. 
505 0 |a pt. 1. Genetic and culture methods. Basic culture methods / James A. Lewis, John T. Fleming -- Mutagenesis / Philip Anderson -- Reverse genetics : from gene sequence to mutant worm / Ronald H.A. Plasterk -- Genetic mapping with polymorphic sequence-tagged sites / Benjamin D. Williams -- Genetic dissection of developmental pathways / Linda S. Huang, Paul W. Sternberg -- Mosaic analysis / Robert K. Herman -- Genetic balancers / Mark L. Edgley [and others] -- pt 2. Neurobiology. Genetic pharmacology : interactions between drugs and gene products in Caenorhabditis elegans / James B. Rand, Carl D. Johnson -- Methods of studying behavioral plasticity in Caenorhabditis elegans / Timothy N. Gannon, Catharine H. Rankin -- Laser killing of cells in Caenorhabditis elegans / Cornelia I. Bargmann, Leon Avery -- Electrophysiological methods / Leon Avery, David Raizen, Shawn Lockery -- pt. 3. Cell biology and molecular biology. Cell biology of nematode sperm / Steven W. L'Hernault, Thomas M. Roberts -- Blastomere culture and analysis / Lois G. Edgar -- Whole-mount in situ hybridization for the detection of RNA in Caenorhabditis elegans embryos / Geraldine Seydoux, Andrew Fire -- Fluorescence in situ hybridization for the detection of DNA and RNA / Donna G. Albertson, Rita M. Fishpool, Philip S. Birchall -- Immunofluorescence microscopy / David M. Miller, Diane C. Shakes -- Electron microscopy and three-dimensional image reconstruction / David H. Hall. 
505 0 |a Proteins and protein assemblies / Henry F. Epstein, Feizhou Liu -- DNA transformation / Craig Mello, Andrew Fire -- Transcription and translation ; Techniques for analyzing transcription and translation / Michael Krause -- pt. 4. Genomics and informatics. The physical map of the Caenorhabditis elegans genome / Alan Coulson [and others] -- Genomic DNA sequencing methods / Anthony Favello, LaDeana Hillier, Richard K. Wilson -- Large-scale complementary DNA sequencing methods / Lucinda L. Fulton, LaDeana Hillier, Richard K. Wilson -- ACeDB and Macace / Frank H. Eeckman, Richard Durbin -- The Worm Community System, release 2.0 (WCSr2) / Laura M. Shoman [and others] -- Appendix A. Additional information and resources -- Appendix B. Caenorhabditis Genetics Center, laboratory designations -- Appendix C. Gene names and descriptions. 
520 |a The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four primary sections--Genetic and Culture Methods, Neurobiology, Cell and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans research. Because C. elegans is a simple and malleable organism with a small genome and few cell types, it provides an elegant demonstr. 
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700 1 |a Epstein, Henry F.,  |d 1944-  |e editor. 
700 1 |a Shakes, Diane C.,  |e editor. 
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