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Biophysical tools for biologists. Volume 1, In vitro techniques /
Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devo...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Boston : Elsevier,
�2008.
|
| Colección: | Methods in cell biology ;
v. 84. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Part I: History and Reflections; ; Section 1. Solution Methods; ; 1. Binding: A Polemic and Rough Guide; Nichola C. Garbett, Jonathan B. Chaires; ; 2. Linked Equilibria in Regulation of Transcription Initiation; Dorothy Beckett; ; 3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions; Farial A. Tanious, Binh Nguyen, and W. David Wilson; ; 4. Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions; Matthew W. Freyer and Edwin A. Lewis; ; 5. Differential Scanning Calorimetry; Charles H. Spink; ; 6. Analytical Ultracentrifugation: A) Sedimentation Velocity, B) Sedimentation Equilibrium; Tom Laue, Tom Moody, James Cole, and Jeff Lary; ; 7. Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity; Nancy K.
- Burgess, Ann Marie Stanley, and Karen G. Fleming; ; 8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods; Susan Bane and Natasha Shanker; ; 9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA Interactions; Vince J. LiCata and Andy J. Wowor; ; 10. Circular Dichroism and Its Application to the Study of Biomolecules; Stephen R. Martin and Maria J. Schilstra; ; 11. Folding and Stability; Timothy O. Street, Naomi Courtemanche and Doug Barrick; ; 12. Hydrodynamic shape modelling of analytical ultracentrifugation data; Olwyn Byron; ; 13. X-ray and neutron scattering data and their constrained molecular modelling; Stephen J Perkins, Azubuike I. Okemefuna, Anira N. Fernando, Alexandra Bonner, Hannah E. Gilbert and Patricia B. Furtado; ; 14. Structural Investigations into Microtubule-MAP Complexes; Andreas Hoenger and Heinz Gross; ; 15. Rapid Kinetic Techniques; John F.
- Eccleston, Stephen Martin, and Maria J. Schilstra; ; 16. Mutagenic Analysis of the Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model Example; George J. Turner; ; 17. Quantifying DNA-protein interactions by single molecule stretching; Mark C. Williams, Ioulia Rouzina, and Richard L. Karpel; ; 18. Isotopomer-based metabolomic analysis by NMR and mass spectrometry; Andrew N Lane, Teresa W-M. Fan, and Richard M. Higashi; ; 19. Following molecular transitions with single residue spatial; and millisecond time resolution; Inna Shcherbakova, Somdeb Mitra, Robert Beer and Michael Brenowitz; ; 20. Methods and applications of site-directed spin labeling EPR spectroscopy; Candice S. Klug and Jimmy B. Feix; ; 21. Fluorescence Correlation Spectroscopy and its application to the characterization of molecular properties and interactions; Hac�ene Boukari and Dan L. Sackett; ; 22.
- Practical Guide to Osmolytes; Jorg Rosgen and Daniel Harries; ; 23. Stupid Statistics; Joel Tellinghuisen; ; 24. Nonlinear Least Squares Fitting Methods; Michael L. Johnson; ; 25. Methods for Simulating the Dynamics of Complex Biological Processes; Maria J Schilstra, Stephen R. Martin, and Sarah M. Keating; ; 26. Computational Methods for Biomolecular Electrostatics; Feng Dong, Brett Olsen and Nathan A. Baker; ; 27. Ligand effects on the protein ensemble: Unifying the descriptions of ligand binding, local conformational fluctuations, and protein stability; Steven T Whitten, Bertrand Garc�ia-Moreno E., and Vincent J. Hilser; ; 28. Molecular Modeling of Cytoskeletal Proteins; Xiange Zheng and David Sept; ; 29. Mathematical Modeling of Cell Motility; Anders E. Carlsson and David Sept.