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G proteins /

This volume presents biochemical and molecular techniques for the functional characterization of G proteins. Detailed methodologies are described for the production and purification of recombinant G protein subunits, for the manipulation of both sense and antisense G proteins RNA and DNA.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Roche, Patrick C.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1996.
Colección:Methods in neurosciences ; v. 29.
Temas:
Acceso en línea:Texto completo
Texto completo
Texto completo
Tabla de Contenidos:
  • G proteins controlling differentiation, growth, and development : analysis by antisense RNA/DNA technology / Christopher M. Moxham, Hsien-yu Wang, Craig C. Malbon
  • Analysis of adenylylcyclase subspecies gene expression in brain by in situ hybridization histochemistry / Nicole Mons
  • Site-directed mutagenesis of G protein [alpha] subunits : analysis of [alpha]₂ phosphorylation and fatty acid acylation / Hazem Hallak, Karen M. Lounsbury, and David R. Manning
  • G protein activity in solubilized membrane preparations / Irene Litosch and Hong Xie
  • Cross-linking of synaptoneurosome G proteins / Sherry Coulter, Saleem Jahangeer, and Martin Rodbell
  • Quantitative reconstitution and ADP-ribosylation assays for G proteins / Joel Abramowitz
  • Functional assays to study coupling of dopamine D2 receptors to G proteins / Ja-Hyun Baik [and others]
  • Assays for G protein [beta]y subunit activity / Joyce B. Higgins and Patrick J. Casey
  • [alpha]₂-adrenergic receptor coupling to G proteins / Ann E. Remmers
  • Somatostatin receptor coupling to G proteins / John R. Hadcock and Joann Strnad
  • Galanin receptor-G protein interactions and stimulation of GTPase activity / Susan L. Gillison, Gunnar Skoglund, and Geoffrey W.G. Sharp
  • Cloning and site-directed mutagenesis studies of gonadotropin-releasing hormone receptor / Stuart C. Sealfon [and others]
  • Use of the Xenopus laevis expression system for structure-function analysis of G-protein-coupled receptor for parathyroid hormone and parathyroid hormone-related protein / Paul R. Turner [and others]
  • Production of G protein [alpha]-subunit-specific monoclonal antibodies / Xiaohua Li and Richard S. Jope
  • Expression and purification of G protein [beta]y subunits using baculovirus expression system / Stephen G. Graber, Margaret A Lindorfer, and James C. Garrison
  • Analysis of G protein [gamma] subunits using baculovirus expression system : requirement for posttranslational processing / Janet D. Robishaw
  • Expression, purification, and functional reconstitution of recombinant phospholipase C-[beta] isozymes / Andrew Paterson and T. Kendall Harden
  • Measuring cooperative binding between retinal rod G protein, transducin, and activated rhodospin / Barry M. Willardson, Tatsuro Yoshida, and Mark W. Bitensky
  • Crosstalk between tyrosine kinase and G-protein-linked signal transduction systems / Ross D. Feldman and Michel Bouvier
  • Subcellular distribution and posttranslational modifications of GTP-binding proteins in insulin-secreting cells / Anjaneyulu Kowluru and Stewart A. Metz
  • Epidermal growth factor-mediated regulation of G proteins and adenylylcylase in cardiac muscle / Tarun B. Patel [and others]
  • G protein dependence of [alpha]₁-adrenergic receptor subtype action in cardiac myocytes / Vitalyi Rybin, Hyung-Mee Han, and Susan F. Steinberg
  • G[subscript s][alpha] mutations and pituitary adenomas / Joseph M. Alexander
  • Molecular methods for analysis of genetic polymorphisms : application to the molecular genetic study of genes encoding [beta]₂-adrenoceptor and stimulatory G protein [alpha] subunit / Charles W. Emala and Michael A. Levine.