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Enzyme kinetics and mechanism. Part E, Energetics of enzyme catalysis /
This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediate...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1999.
|
| Colección: | Methods in enzymology ;
volume 308. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Sect. 1. Energetic coupling in enzymatic reactions
- Energetics of substate binding, catalysis, and product release
- Energy coupling through molecular discrimination: nicotinate phosphoribosyltransferase
- On the advantage of imperfect energetic linkage
- Reaction dynamics of G-protein catalyzed hydrolysis of GFP as viewed by x-ray crystallographic snapshots of Gi[alpha]₁
- Energetics of nucleotide hydrolysis in polymer assembly/disassembly: the cases of actin and tubulin
- Fundamental mechansisms of substrate channeling
- Sect. 2. Intermediates and complexes in catalysis
- Intermediates and energetics in pyruvate phosphate dikinase
- Raman spectroscopic studies of the structures, energetics, and bond disortions of substrates bound to enzymes
- Crystallographic analysis of solvent-trapped intermediates to chymotrypsin
- Sect. 3. Detection and properties of low-barrier hydrogen bonds
- Nuclear magnetic resonance methods for the detection and study of low-barrier hydrogen bonds of enzymes
- Hydrogen bonding in enzymatic catalysis: analysis of energetic contributions
- Application of marcus rate theory to proton transfer in enzyme-catalyzed reactions
- Sect. 4. Transition state determination and inhibitors
- Enzymatic transition-state analysis and transition-state analogues
- Determining transition states from kinetic isotope effects
- Computational methods fro transition state and inhibitor recognition.