Cargando…

Advanced bacterial genetics : Use of transposons and phage for genomic engineering /

A volume of indispensable methods for the use of transposons and phage in genomic engineering.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Hughes, Kelly Thomas (Editor ), Maloy, Stanley R. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego, California : Elsevier Academic Press, �2007.
Colección:Methods in enzymology ; v. 421.
Temas:
Acceso en línea:Texto completo
Texto completo
Texto completo
Tabla de Contenidos:
  • Strain collections and genetic nomenclature / Stanley R. Maloy and Kelly T. Hughes
  • Use of antibiotic-resistant transposons for mutagenesis / Stanley R. Maloy
  • In vivo mutagenesis using EZ-Tn5 / John R. Kirby
  • Identification of essential genes in bacteria / David R. Hillyard and Michael J. Reed
  • Isolation of transposon insertions / Stanley R. Maloy
  • Localized mutagenesis / Stanley R. Maloy
  • Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons / Kelly T. Hughes
  • Target-directed proteolysis in vivo / Markus Eser [and others]
  • Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering / Beth Traxler and Eliora Gachelet
  • Using genomic microarrays to study insertional/transposon mutant libraries / David N. Baldwin and Nina R. Salama
  • Screening transposon mutant libraries using full-genome oligonucleotide microarrays / Kelly M. Winterberg and William S. Reznikoff
  • Creating recombination-activated genes and sequence-defined mutant libraries using transposons / Larry Gallagher [and others]
  • Use of operon and gene fusions to study gene regulation in Salmonella / Kelly T. Hughes and Stanley R. Maloy
  • Genomic screening for regulatory genes using the T-POP transposon / Changhan Lee [and others]
  • Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond / James A. Sawitzke [and others]
  • [lambda]-red genetic engineering in Salmonella enterica serovar Typhimurium / Joyce E. Karlinsey
  • Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements / Nathalie Garcia-Russell, Samantha S. Orchard, and Anca M. Segall
  • Dissecting nucleic acid-protein interactions using challenge phage / Stanley R. Maloy and Jeffrey Gardner
  • Mud-P22 / Jenny A. Crain and Stanley R. Maloy
  • Phage metagenomics / Veronica Casas and Forest Rowher.