Guide to techniques in mouse development /

This volume provides, for the first time, an extensive compilation of those state-of-the-art methods that have revolutionized the use of mice as an experimental system. This volume is a unique source for detailed descriptions to be used in research by both experienced and novice researchers - Germ c...

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Bibliographic Details
Call Number:Libro Electrónico
Other Authors: Wassarman, Paul M. (Editor), DePamphilis, Melvin L. (Editor)
Format: Electronic eBook
Language:Inglés
Published: San Diego : Academic Press, �1993.
Series:Methods in enzymology ; v. 225.
Subjects:
Molecular biology > Technique.
Mice as laboratory animals.
Developmental biology.
Genetics > Technique.
Gene expression.
Mice > Development.
Mice > genetics
Mice > embryology
Genetic Techniques
Gene Expression
Developmental Biology
Souris > D�eveloppement.
Biologie mol�eculaire > Technique.
Souris (Animal de laboratoire)
Biologie du d�eveloppement.
G�en�etique > Technique.
Expression g�enique.
Mice > Development
Genetics > Technique
Gene expression
Developmental biology
Mice as laboratory animals
Molecular biology > Technique
Laboratory animals
Online Access:Texto completo
Texto completo
Table of Contents:
  • Section I. General methodology
  • Section II. Germ cells and embryos
  • Section III. Fertilization
  • Section IV. Gene expression : Messenger RNA
  • Section V. Gene expression : Reporter genes
  • Section VI. Gene expression : Proteins
  • Section VII. Gene expression : Methylation
  • Section VIII. Gene identification
  • Section IX. Nuclear transplantation
  • Section X. Transgenic animals : Pronuclear injection
  • Section XI. Transgenic animals : Embryonic stem cells and gene targeting
  • Section XII. Lineage analysis.

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