Overview of blotting techniques

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Sripathi, Shiv Sanjeevi, Pandey, Prerna
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Ashland : Delve Publishing, 2020.
Temas:
Immunoblotting.
Western immunoblotting.
Macromolecules.
Technique des immunoempreintes.
Buvardage de western.
Immunoblotting
Macromolecules
Western immunoblotting
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Cover
  • Copyright
  • DECLARATION
  • ABOUT THE EDITORS
  • TABLE OF CONTENTS
  • List of Contributors
  • List of Abbreviations
  • Preface
  • Chapter 1 Western Blotting-the Works
  • 1.1 Concept
  • 1.2 The Details
  • 1.3 General Protocol For Western Blotting
  • 1.4 Troubleshooting
  • 1.5 Advances In Western Blot
  • 1.6 Final Word
  • 1.7 References
  • Chapter 2 Southern Blotting and Associated Techniques
  • 2.1 Introduction
  • 2.2 Interesting Insights: The Role of Dr. Southern In Science
  • 2.3 Technology Types
  • 2.4 The Southern Blotting Principle
  • 2.5 Recent Alterations To Southern Blotting
  • 2.6 Dna Pre-Preparation Before Southern Blotting
  • 2.7 Techniques For Dna Blotting
  • 2.8 The Southern Blot Prehybridization
  • 2.9 Washing Steps and Hybridization
  • 2.10 Role of Southern Blotting In Identification of Clones
  • 2.11 Polymorphism Analysis of Restriction Fragment Length
  • 2.12 Final Words
  • 2.13 References
  • Chapter 3 Northern Blotting: A Brief Overview
  • 3.1 Introduction
  • 3.2 The Procedure In Brief
  • 3.3 Applications Of The Northern Blotting Technique
  • 3.4 Drawbacks Of The Northern Blotting Technique (Taniguchi Et Al., 2001
  • Karen Reue, 1998)
  • 3.5 The Reverse Northern Blot Technique
  • 3.6 The Northwestern Blot
  • 3.7 Summary
  • 3.8 References
  • Chapter 4 Eastern Blotting
  • 4.1 Outline
  • 4.2 Case Study 1: Eastern Blotting For A Herb Vs. Tlc (Thin Layer Chromatography)
  • 4.3 Case Study 2: Eastern Blotting For Bioactive Compounds
  • 4.4 References
  • Chapter 5 Misleading Westerns: Common Quantification Mistakes In Western Blot Densitometry And Proposed Corrective Measures
  • Abstract
  • Introduction
  • Materials And Methods
  • Results
  • Discussion
  • Conclusions
  • Data Availability
  • Conflicts Of Interest
  • Acknowledgments
  • Careful Experimental Design Produces Reliable And Reproducible Data
  • Sample Preparation
  • Determine The Linear Dynamic Range of Protein Loading
  • Determine The Appropriate Reference Signal For Data Normalization
  • Data Analysis: The Background Subtraction Problem
  • Computational Analysis of Densitometric Data
  • Conclusions
  • Abbreviations
  • Conflict Of Interests
  • References
  • Chapter 9 Characterization Of Nora Virus Structural Proteins Via Western Blot Analysis
  • Abstract
  • Introduction
  • Materials And Methods
  • Discussion
  • Conclusion
  • Competing Interests

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