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Electrophoresis in practice : a guide to methods and applications of DNA and protein separations /
The fifth edition of this successful text has been revised and expanded, omitting some topics on obsolete DNA electrophoresis. It features a section on electrophoretic micro-methods and on-the-chip electrophoresis. It is geared towards advanced students and professionals and contains extended backgr...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Weinheim, Germany :
Wiley-VCH,
[2016]
|
| Edición: | Fifth edition. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Matter
- Part I: Fundamentals. Fundamentals
- Electrophoresis
- Isotachophoresis
- Isoelectric Focusing
- High-Resolution Two-Dimensional Electrophoresis
- Protein Sample Preparation
- Protein Detection
- Blotting
- Part II: Equipment and Methods. Equipment and Methods
- Special Laboratory Equipment
- Consumables
- Chemicals
- Method 1: PAGE of Dyes
- Method 2: Agarose and Immunoelectrophoresis
- Method 3: Titration Curve Analysis
- Method 4: Native PAGE in Amphoteric-Buffers
- Method 5: Agarose IEF
- Method 6: PAGIEF in Rehydrated Gels
- Method 7: Horizontal SDS-PAGE
- Method 8: Vertical PAGE
- Method 9: Semidry Blotting of Proteins
- Method 10: IEF in Immobilized pH Gradients
- Method 11: High-Resolution 2D Electrophoresis
- Method 12: PAGE of DNA Fragments
- Appendix: Troubleshooting.