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RNA Methodologies : a Laboratory Guide for Isolation and Characterization.
The expanded third edition of this highly successful laboratory manual presents new technologies in RNA interference.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Burlington :
Elsevier,
2005.
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| Edición: | 3rd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front cover; Title page; Copyright page; Dedication; Table of contents; Preface; 1 RNA and the Cellular Biochemistry Revisited; 2 Transcription and the Organization of Eukaryotic Genes; 3 Messenger RNA; 4 Resilient Ribonucleases; 5 RNA Isolation Strategies; 6 The Truth About Tissues; 7 Isolation of Polyadenylated RNA; 8 Quality Control for RNA Preparations; 9 Dot Blot Analysis; 10 Electrophoresis of RNA; 11 Photodocumentation and Image Analysis; 12 Northern Analysis; 13 Nucleic Acid Probe Technology; 14 Practical Nucleic Acid Hybridization; 15 Principles of Detection.
- 16 Quantification of Specific mRNAs by Nuclease Protection17 Analysis of Nuclear RNA; 18 cDNA Synthesis; 19 RT-PCR; 20 Quantitative PCR Techniques; 21 Transcript Subtraction Methods; 22 mRNA Differential Display; 23 High-Throughput Analysis of Gene Expression; 24 RNA Interference: Targeted Gene Silencing; 25 Genomes, Transcriptomes, Proteomes, and Bioinformatics; 26 An RNA Paradigm; Epilogue: A Few Pearls of Wisdom; A Maintaining Complete and Accurate.