Imaging in virus research /

Imaging in Virus Research, Volume 116 in the Advances in Virus Research series highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Topics of interest in this release include Recent developments of advanced fluoresce...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Finke, Stefan, Ushakov, Dmitry
Formato: Electrónico eBook
Idioma:Inglés
Publicado: [Place of publication not identified] : Academic Press, 2023.
Colección:Advances in virus research ; volume 116
Temas:
Virus diseases > Imaging.
Diagnostic virology.
Maladies �a virus > Imagerie.
Diagnostics virologiques.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • Imaging in Virus Research
  • Copyright
  • Contents
  • Contributors
  • Chapter One: Spatial resolution of virus replication: RSV and cytoplasmic inclusion bodies
  • 1. Introduction
  • 1.1. Respiratory syncytial virus
  • 1.2. Respiratory syncytial virus: An unmet medical need
  • 1.3. RSV multiplication is still mysterious
  • 1.3.1. Entry steps
  • 1.3.2. Viral RNA synthesis
  • 1.3.3. Export and assembly
  • 1.4. IBs: Mysterious virally-induced compartment
  • 2. Characterizing RSV IBs
  • 2.1. Fluorescently labeled IBs
  • 2.2. Uncovering the LLPS nature of IBs
  • 2.3. Identifying the minimal components/domains required to induce IBs formation
  • 2.4. Demonstrating that IBs are viral factories
  • 2.5. Probing IBs internal organization
  • 2.6. Cellular proteins recruited in IBs
  • 3. Conclusions
  • References
  • Further reading
  • Chapter Two: From the beginnings to multidimensional light and electron microscopy of virus morphogenesis
  • 1. Introduction: Imaging viral morphogenesis in three dimensions (3D)
  • 1.1. Seeing is believing: Fluorescent tags and live-cell imaging in studying viral Infections
  • 1.2. Considerations for studying viral morphogenesis with volumetric live-cell microscopy
  • 1.3. Widefield and confocal fluorescence microscopy for volumetric live-cell microscopy
  • 1.4. Light sheet microscopy
  • 1.5. Super-resolution light microscopy
  • 2. Imaging viral morphogenesis in 3D using EM
  • 2.1. EM at the forefront of virus research
  • 2.1.1. The development of EM
  • 2.1.2. Development of biological sample preparation methods for EM
  • 2.2. Studying native samples: Cryo-EM
  • 2.3. Study of viruses using EM
  • 2.4. Moving beyond the second dimension: 3D-EM
  • 2.5. Volumetric imaging with FIB milling and serial block face SEM (SBF-SEM)
  • 3. Correlative light- and electron microscopic approaches for imaging viral morphogenesis
  • 3.1. Correlating light microscopy with SBF-SEM
  • 3.2. Correlating light microscopy with Cryo-EM
  • 4. Novel methods and future directions
  • 4.1. Volumetric (correlative) cryo super-resolution microscopy
  • 4.2. Bioluminescent imaging
  • References
  • Chapter Three: Tissue optical clearing and 3D imaging of virus infections
  • 1. Introduction-3D fluorescence imaging
  • 2. 3D imaging of optically cleared tissues in virus research
  • 3. 3D tissue imaging of virus vectors for neuronal tracing, gene- and oncolytic therapy
  • 4. 3D tissue imaging in virus and pathogenicity research
  • 4.1. Human immunodeficiency virus (HIV)
  • 4.2. Respiratory syncytial virus (RSV)
  • 4.3. Rabies virus (RABV)
  • 4.4. SARS-CoV-2
  • 5. 3D image analysis
  • 6. Conclusions
  • 7. Outlook
  • Acknowledgment
  • References
  • Chapter Four: Advanced fluorescence microscopy in respiratory virus cell biology
  • 1. Introduction

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