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Modifications and targeting of protein termini. Part A /
Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltr...
| Clasificación: | Libro Electrónico |
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| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Academic Press,
2023.
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| Colección: | Methods in enzymology ;
v. 684 |
| Temas: | |
| Acceso en línea: | Texto completo |
| Sumario: | Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more. Additional sections cover Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1 as an autophagic N-recognin of the Arg/N-degron pathway, Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, and so much more. |
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| Notas: | <p>1. Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells <i>Bernd Bukau, Jiri Koubek, Manuel G�unnigmann and G�unter Kramer </i>2. Deformylation of nascent chains on the ribosome <i>Marina V. Rodnina, Lena B�ogeholz, Evan Mercier and Wolfgang Wintermeyer </i>3. Optimizing purification and activity assays of N-terminal methyltransferase complexes <i>Christine Schaner Tooley, Haley Parker and John Tooley </i>4. Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods <i>Rong Huang and Ying Meng </i>5. Biochemical and structural analysis of N-myristoyltransferase-mediated protein tagging <i>Thierry Meinnel, Carmela Giglione, Paul Monassa, Fr�ed�eric Rivi�ere, Cyril Dian and Fr�ed�eric Frottin </i>6. Kinetic and catalytic features of N-myristoyltransferases <i>Thierry Meinnel and Carmela Giglione </i>7. Detecting N-terminal myristoylation using metabolic Alk12 labeling <i>Hening Lin and Yilai Xu </i>8. Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activities <i>Iris Finkemeier, J�urgen Eirich, Julia Sindlinger, Stefan Sch�on and Dirk Schwarzer </i>9. A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms <i>Kris Gevaert, Annelies Bogaert, Tessa Van de Steene, Marnik Vuylsteke and Sven Eyckerman </i>10. The amino-dipeptidyl peptidases DPP8 and DPP9: purification, interaction studies and enzymatic assays <i>Ruth Geiss-Friedlander, Laura Donzelli and Oguz Bolgi</p></i> |
| Descripción Física: | 1 online resource (412 pages). |
| ISBN: | 9780443157738 0443157731 |