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Targeted protein degradation /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Burslem, George L. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cambridge : Academic Press, 2023.
Colección:Methods in enzymology ; v. 681.
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • One - High-efficiency knock-in of degradable tags (dTAG) at endogenous loci in cell lines
  • Two - High throughput E3 ligase degron binding assays for novel PROTAC ligand discovery
  • Three - Synthesis of SNIPERs against BCR-ABL with kinase inhibitors and a method to evaluate their growth inhibitory activity derived from BCR-ABL degradation
  • Four - Harnessing nanobodies for target protein degradation through the Affinity-directed PROtein Missile (AdPROM) system
  • Five - Monitoring PROTAC interactions in biochemical assays using Lumit immunoassays
  • Six - The In-Cell Western immunofluorescence assay to monitor PROTAC mediated protein degradation
  • Seven - Cereblon target validation using a covalent inhibitor of neosubstrate recruitment
  • Eight - Advancing targeted protein degrader discovery by measuring cereblon engagement in cells
  • Nine - Quantitative measurement of PROTAC intracellular accumulation
  • Ten - The importance of controls in targeted protein degradation: Determining mechanism
  • Eleven - Crystallization of VHL-based PROTAC-induced ternary complexes
  • Twelve - Targeted protein degradation through light-activated E3 ligase recruitment
  • Thirteen - A heterobifunctional molecule system for targeted protein acetylation in cells