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Integrated methods in protein biochemistry. Part A /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cambridge, MA :
Academic Press,
2022.
|
| Colección: | Methods in enzymology ;
v. 675. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- 1. Engineering protein and DNA tools for creating DNA-dependent protein switches / Harsimranjit Sekhon, Jeung-Hoi Ha, and Stewart N. Loh
- 2. Reconstitution and biochemical studies of extended synaptotagmin-mediated lipid transport / Ruyue He, Chenlu Li, Yinghui Liu, and Haijia Yu
- 3. Synthesis of O-GlcNAcylated small heat shock proteins / Stuart P. Moon and Matthew R. Pratt
- 4. Druggable sites/pockets of the p53-DNAJA1 protein-protein interaction: In silico modeling and in vitro/in vivo validation / Danielle Jacobsen, Omar Bushara, Rama K. Mishra, Leyu Sun, Jie Liao, and Guang-Yu Yang
- 5. RAPIDS, a method for sub-compartmental identification of protein interactomes / Christina James, Christof Lenz, and Ralph H. Kehlenbach
- 6. Purification and biochemical characterization of the Rag GTPase heterodimer / Dylan D. Doxsey and Kuang Shen
- 7. Isolation of synthesized E. coli elongation complexes for solid-phase and solution-based in vitro transcription assays / Eric J. Strobel, Skyler L. Kelly, and Courtney E. Szyjka
- 8. Cellular experiments to study the inhibition of c-Myc/MAC heterodimerization / Ashutosh Singh, Shilpa Sharma, Praveen Kumar, and Neha Garg
- 9. Fluorogenic RNA aptamers to probe transcription initiation and co-transcriptional RNA folding by multi-subunit RNA polymerases / Yong-Heng Huang, Vilma Trapp, Oskari Puro, Janne J. M�akinen, Mikko Mets�a-Ketel�a, Markus C. Wahl, and Georgiy A. Belogurov
- 10. Phosphorylation and subcellular localization of human phospholipase A1, DDHD1/PA-PLA1 / Atsushi Yamashita, Naoki Matsumoto, Yoko Nemoto-Sasaki, Saori Oka, Seisuke Arai, and Ikuo Wada
- 11. Elucidation of folding pathways of knotted proteins / Sarita Puri and Shang-Te Danny Hsu
- 12. Biophysical and structural characterizations of the effects of mutations on the structure-activity relationships of SARS-CoV-2 spike protein / Pei-Yu Yu, Tzu-Jing Yang, and Shang-Te Danny Hsu
- 13. A gel electrophoresis-based assay for measuring enzymatic RNA decapping activity / Yogeshwari Singh and Jeremy G. Bird
- 14. Nanoluciferase-based cell fusion assay for rapid and high-throughput assessment of SARS-CoV-2-neutralizing antibodies in patient samples / Max Meyrath, Martyna Szpakowska, Jean-Marc Plesseria, Olivia Domingues, J�er�emie Langlet, Bernard Weber, Rejko Kr�uger, Markus Ollert, and Andy Chevign�e
- 15. Linchpin-directed precise labeling of lysine in native proteins, purification, and analysis / Ankita Bal, Shivam Kumar Singh, Tanushree Kashyap, and Vishal Rai
- 16. In vitro and in cellular methods for determining the target protein SUMOylation / Sajeev T.K., Aswini Kumar Panda, Jyotsna Kawadkar, Divya Bindra, Pradyumna A. Joshi, Garima Joshi, Harsh Vardhan Singh, and Ram Kumar Mishra
- 17. Two-dimensional electrophoresis-cellular thermal shift assay (2DE-CETSA) for target identification of bioactive compounds / Makota Muroi and Hiroyuki Osada..